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Citations et références (8)
Invitrogen™
3-Amino-3-Deoxydigoxigenin Hemisuccinamide, Succinimidyl Ester
Hémisuccinamide de 3-amino-3-désoxydigoxigénine, ester de succinimidyle
| Référence | Quantité |
|---|---|
| A2952 également connu sous le numéro A-2952 | 5 mg |
Référence A2952
également connu sous le numéro A-2952
Prix (EUR)
516,00
Each
Quantité:
5 mg
Prix (EUR)
516,00
Each
La version réactive aux amines de la digoxigénine (hémisuccinamide de 3-amino-3-désoxygigoxigénine, SE) peut être utilisée pour attacher cet haptène important aux biomolécules d’intérêt pour une détection ultérieure avec un anticorps anti-digoxigénine.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Réactivité chimiqueAmine
Marqueur ou colorantDigoxigénine
Type de produitHémisuccinamide de 3-amino-3-désoxydigoxigénine, ester de succinimidyle
Quantité5 mg
Groupement de réactifsEster actif, ester de succinimidyle
Conditions d’expéditionTempérature ambiante
Type d’étiquetteBiotine et autres haptènes
Unit SizeEach
Contenu et stockage
Conserver au congélateur (-5 à -30°C).
Citations et références (8)
Citations et références
Abstract
Erratic deposition of agrin during the formation of Xenopus neuromuscular junctions in culture.
Journal:Dev Biol
PubMed ID:7601300
'In order to disclose the mechanism that regulate synapse development we compared the distributions of agrin, acetylcholine receptors (AChR) and a basal lamina heparan sulfate proteoglycan (HSPG) in sections and cultures prepared from Xenopus laevis and Ambystoma mexicanum embryos. While agrin, AChR and HSPG may accumulate almost synchronously at synapses
A nonradioactive DNA methyltransferase assay adaptable to high-throughput screening.
Journal:Anal Biochem
PubMed ID:15840507
'We have developed a nonradioactive assay method for DNA methyltransferases based on the ability to protect substrate DNA from restriction. DNA immobilized to a microplate well was treated sequentially with methyltransferase and an appropriate endonuclease. The amount of methylated DNA product is reflected by a proportional decrease in endonuclease cleavage,
Characterization of protein-hapten conjugates. 2. Electrospray mass spectrometry of bovine serum albumin-hapten conjugates.
Journal:Bioconjug Chem
PubMed ID:8853461
Fifteen hapten-bovine serum albumin (BSA) conjugates were prepared from five commercially available activated haptens. Each hapten was coupled to BSA at three different ratios. The conjugates were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two mass spectrometry (MS) methods: matrix-assisted laser desorption ionization (MALDI) and liquid chromatography-electrospray
Custom fluorescent-nucleotide synthesis as an alternative method for nucleic acid labeling.
Journal:Nat Biotechnol
PubMed ID:10700155
The variety of potentially useful dyes or haptenes available for fluorescent nucleic acid hybridization assays is far greater than what can be obtained from commercial sources. Since this diversity could be useful in many laboratory applications, we have developed a simple and inexpensive procedure for preparing nonpurified labeled nucleotides, for
Mycobacterium avium- and Mycobacterium tuberculosis-containing vacuoles are dynamic, fusion-competent vesicles that are accessible to glycosphingolipids from the host cell plasmalemma.
Journal:J Immunol
PubMed ID:8648123
The vacuoles inhabited by viable Mycobacterium avium and Mycobacterium tuberculosis show limited fusion with endosomal and lysosomal compartments. This ability to regulate the maturation of their phagosomal compartments and restrict their differentiation into hydrolytically active vacuoles appears to correlate with the survival of the bacilli. Data presented in this current