Alexa Fluor™ 488 5-SDP Ester (Alexa Fluor™ 488 Sulfodichlorophenol Ester)

Citations et références (12)

Invitrogen™

Alexa Fluor™ 488 5-SDP Ester (Alexa Fluor™ 488 Sulfodichlorophenol Ester)

L’ester SDP (sulfodicholorphénol) est actuellement la fraction réactive à l’amine la plus stable par hydrolyse disponible chez Invitrogen. Les conjuguésAfficher plus

Référence	Quantité
A30052	1 mg

Référence A30052

Prix (EUR)

550,00

Ajouter au panier

Quantité:

Prix (EUR)

550,00

Ajouter au panier

L’ester SDP (sulfodicholorphénol) est actuellement la fraction réactive à l’amine la plus stable par hydrolyse disponible chez Invitrogen. Les conjugués produits avec l’ester Alexa Fluor™ 488 5-SDP produisent la même forte liaison amide entre le colorant et le composé d’intérêt que les esters succinimidyle (SE ou NHS) et tétrafluorophényle (TFP), mais avec une meilleure stabilité dans l’eau et les tampons, l’ester SDP peut potentiellement offrir un contrôle et une cohérence accrus dans ces réactions par rapport aux esters NHS et TFP.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Réactivité chimiqueAmine

Marqueur ou colorantAlexa Fluor™ 488

Type de produitEster sulfodichlorophénol

Quantité1 mg

Groupement de réactifsEster actif, ester SDP

Conditions d’expéditionTempérature ambiante

Type d’étiquetteColorants Alexa Fluor

Gamme de produitsAlexa Fluor

Unit SizeEach

Contenu et stockage

Conserver au congélateur (-5 à -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (12)

Citations et références

Abstract

Evidence that viral RNAs have evolved for efficient, two-stage packaging.

Authors:Borodavka A, Tuma R, Stockley PG,

Journal:Proc Natl Acad Sci U S A

PubMed ID:23019360

'Genome packaging is an essential step in virus replication and a potential drug target. Single-stranded RNA viruses have been thought to encapsidate their genomes by gradual co-assembly with capsid subunits. In contrast, using a single molecule fluorescence assay to monitor RNA conformation and virus assembly in real time, with two ... More

Toxicity of organic fluorophores used in molecular imaging: literature review.

Authors:Alford R, Simpson HM, Duberman J, Hill GC, Ogawa M, Regino C, Kobayashi H, Choyke PL,

Journal:Mol Imaging

PubMed ID:20003892

'Fluorophores are potentially useful for in vivo cancer diagnosis. Using relatively inexpensive and portable equipment, optical imaging with fluorophores permits real-time detection of cancer. However, fluorophores can be toxic and must be investigated before they can be administered safely to patients. A review of published literature on the toxicity of ... More

Optical properties of Alexa 488 and Cy5 immobilized on a glass surface.

Authors:Wang L, Gaigalas AK, Reipa V

Journal:Biotechniques

PubMed ID:15679095

'The absorption and emission spectra were measured for Cy5 and Alexa 488 fluorophores confined on a glass surface. The data were obtained using fluorometry and spectroscopic ellipsometry. Red shifts of the surface-immobilized fluorophore absorption spectra relative to the fluorophore spectra in aqueous solution were observed using both methods. We interpret ... More

Modulation of the Pyrococcus abyssi NucS endonuclease activity by replication clamp at functional and structural levels.

Authors:Creze C, Ligabue A, Laurent S, Lestini R, Laptenok SP, Khun J, Vos MH, Czjzek M, Myllykallio H, Flament D,

Journal:J Biol Chem

PubMed ID:22431731

'Pyrococcus abyssi NucS is the founding member of a new family of structure-specific DNA endonucleases that interact with the replication clamp proliferating cell nuclear antigen (PCNA). Using a combination of small angle x-ray scattering and surface plasmon resonance analyses, we demonstrate the formation of a stable complex in solution, in ... More

Milatuzumab-conjugated liposomes as targeted dexamethasone carriers for therapeutic delivery in CD74+ B-cell malignancies.

Authors:Mao Y, Triantafillou G, Hertlein E, Towns W, Stefanovski M, Mo X, Jarjoura D, Phelps M, Marcucci G, Lee LJ, Goldenberg DM, Lee RJ, Byrd JC, Muthusamy N,

Journal:Clin Cancer Res

PubMed ID:23209030

'Corticosteroids are widely used for the treatment of B-cell malignancies, including non-Hodgkin lymphoma, chronic lymphocytic leukemia (CLL), and acute lymphoblastic leukemia; however, this class of drug is associated with undesirable off-target effects. Herein, we developed novel milatuzumab-conjugated liposomes as a targeted dexamethasone carrier for therapeutic delivery in CD74(+) B-cell malignancies ... More

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