Sérum de veau fœtal, qualifié, États-Unis

Name: Sérum de veau fœtal, qualifié, États-Unis
SKU: A3160502

Les sérums de veau fœtal Gibco offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée etAfficher plus

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Référence	Quantité	Format
A3160502	10 x 50 mL	One Shot
A3160501	50 mL	One Shot
26140079	500 mL	Flacon

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Our improved Premium Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.

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Les sérums de veau fœtal Gibco offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée et les dosages spécifiques. Ils bénéficient de la confiance des chercheurs grâce à un soutien primé et d’une qualité constante, répondant à vos besoins en matière de recherche et de budget

Sérums Gibco : Qualité fiable pour une performance constante

Catégorie du sérum Gibco : Valeur plus élevée (performance)
• À utiliser pour la culture cellulaire générale avec des lignées cellulaires courantes
• Niveaux faibles d’endotoxine et d’hémoglobine
• Niveau d’endotoxine : < 10 UE/ml
• Niveau d’hémoglobine : < 25 mg/dl
• Origine : États-Unis

Les sérum Gibco sont
• Certifiés ISO 13485 et traités dans des installations homologuées par la FDA
• Marqués pour le diagnostic in vitro (conformes aux niveaux de contrôles de stérilité USP les plus élevés)
• Filtrés trois fois à 0,1 µm
• Le flacon Gibco est plus facile à utiliser dans la hotte, réduisant le risque de contamination et vous aidant à réaliser une culture cellulaire plus régulière
• L’autocollant détachable sur l’étiquette fournit une référence pratique permettant d’enregistrer le numéro de lot et la date de péremption dans le cahier de laboratoire

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

Spécifications

DescriptionSérum de veau fœtal, qualifié, format One Shot™, États-Unis

Concentration en endotoxines≤10 EU/mL

Concentration d’hémoglobine≤ 25 mg/dl

Gamme de produitsOne Shot

Pureté ou qualitéQualifié

Conditions d’expéditionCongelé

EspècesBovins

AgeFœtal

Pays d’origineÉtats-Unis

FormeLiquide

FormatOne Shot

Type de produitSérum de veau fœtal

Quantité10 x 50 mL

Serum TreatmentStandard (filtration stérile)

StérilitéStérile

Sterilization MethodTriple-filtered, 0.1 μm

Unit SizeEach

Contenu et stockage

Conditions de stockage : ≤-10°C
Conditions d’expédition : Congelé

Foire aux questions (FAQ)

Why would I heat inactivate serum?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat inactivated serum is recommended include immunological studies and culturing ES cells, insect cells, and smooth muscle cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I heat inactivate serum?

Heat at 56 degrees C in a water bath for 30 minutes. Swirl bottle every 10 minutes or so. For accuracy, use a second bottle of similar size and add the equivalent volume of water to this bottle. Place a thermometer in this control bottle in order to see when 56 degrees C is reached. Set your timer for 30 minutes at this point.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

What is the shelf life of Fetal Bovine Serum, qualified, United States once thawed? Can I refreeze it after thawing?

The stability after opening and thawing is application-dependent and most often, it is used within a week or less. The product can be thawed and refrozen. This is standard practice for making aliquots. We recommend that you make smaller aliquots and use as needed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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