TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 0.1 mL

The TaqMan Arbovirus Triplex Kit enables the simultaneous detection and differentiation of RNA from dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) in urine and serum. It is a lyophilized, multiplex, one-step real-time PCR Applied Biosystems TaqMan assay for the molecular detection of dengue, chikungunya, and Zika RNA. The kit is optimized for use with the Applied Biosystems QuantStudio 5 Real-Time PCR System, but is also suitable for the 7500, 7500 Fast, and other QuantStudio systems. It includes a primer-probe set for human PPIA (cyclophilin A) as an endogenous extraction, RT-PCR and PCR amplification control. The kit is compatible with RNA extracted from human urine or serum using the MagMAX Pathogen RNA/DNA Kit. The Zika virus sequence used to develop these primers and probes is from a 2014 research paper by Pyke et al (1).

Features of the kit:
• Lyophilized with one-step RT-PCR master mix; simply add RNA
• Simple workflow for detection of Zika, Dengue, and Chikungunya virus RNA from urine or serum
• Easy to use and environmentally friendly. No cold chain management required; shipped and stored at room temperature.

Contents:
• Twelve 8-well strips tubes for 96 total reactions per kit
• 0.1-mL tube format, compatible with 96-well fast block
• Primer-probe detection with 4 probe reporter dyes:
   --FAM-Zika
   --VIC-Dengue
   --ABY-Chikungunya
   --JUN-PPIA cyclophilin

1. Pyke AT, Daly MT, Cameron JN, Moore PR, Taylor CT, Hewitson GR, Humphreys JL, Gair R. Imported zika virus infection from the cook islands into australia, 2014. PLoS Curr. 2014 Jun 2;6.