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Sérum de veau fœtal, qualifié, inactivé par la chaleur, États-Unis
Gibco™

Sérum de veau fœtal, qualifié, inactivé par la chaleur, États-Unis

Les sérums de veau fœtal Gibco offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée etAfficher plus
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RéférenceQuantitéFormat
A384010210 x 50 mLOne Shot
A384010150 mLOne Shot
16140071500 mLFlacon
Our improved Premium Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Référence A3840102
Les sérums de veau fœtal Gibco offrent une excellente valeur pour la culture cellulaire de base, la recherche spécialisée et les dosages spécifiques. Ils bénéficient de la confiance des chercheurs grâce à un soutien primé et d’une qualité constante, répondant à vos besoins en matière de recherche et de budget

Catégorie du sérum : Valeur plus élevée (performance)
Origine : États-Unis.
Niveau d’endotoxine : ≤ 10 UE/ml
Niveau d’hémoglobine : ≤ 25 mg/dl.
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Spécifications
DescriptionSérum de veau fœtal, inactivé par la chaleur, qualifié, One Shot™, États-Unis
Concentration en endotoxines≤ 10 UE/mL
Inactivé par la chaleurYes
Concentration d’hémoglobine≤ 25 mg/dl
TraitementTriple filtration à 0,1 μm
Gamme de produitsOne Shot
Pureté ou qualitéQualifié
Durée de conservation5 Years
Conditions d’expéditionCongelé
EspècesBovins
AgeFœtal
Pays d’origineÉtats-Unis
FormeLiquide
FormatOne Shot
Type de produitSérum de veau fœtal
Quantité10 x 50 mL
Serum TreatmentInactivé par la chaleur
StérilitéStérile
Sterilization MethodTriple filtration, 0,1 μm
Unit SizeEach
Contenu et stockage
Conditions de stockage : ≤-10°C
Conditions d’expédition : Congelé

Foire aux questions (FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

When should I use heat-inactivated serum?

Heat-inactivation of serum inactivates the “complements” in the serum. In general, it is not necessary to use heat-inactivated serum to culture most cell types. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I would like to order heat-inactivated FBS. What conditions do you use for heat-inactivation of FBS during the manufacturing process?

Serum is heat inactivated by heating at 56 degrees C in a water bath for 30 min, swirling the bottle approximately every 10 minutes.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of Fetal Bovine Serum, qualified, United States once thawed? Can I refreeze it after thawing?

The stability after opening and thawing is application-dependent and most often, it is used within a week or less. The product can be thawed and refrozen. This is standard practice for making aliquots. We recommend that you make smaller aliquots and use as needed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between "qualified" versus "certified" purity grade FBS?

The main difference is in the Quality Control (QC) test specifications. “Qualified” and “Certified” FBS go through the same QC tests but “Certified” FBS has additional QC tests as well as more stringent QC test specifications for endotoxin and hemoglobin levels. The additional QC tests in “Certified” FBS include biochemical and hormonal profiling.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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