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Applied Biosystems™

TaqMan™ Microbe Detection Assay, pooled

Applied Biosystems TaqMan Microbe Detection assays are used for quantitative real-time PCR detection of microbial species. These assays consist ofAfficher plus
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RéférenceQuantité
A52083250 μl
Référence A52083
Prix (EUR)
302,00
Each
Quantité:
250 μl

Applied Biosystems TaqMan Microbe Detection assays are used for quantitative real-time PCR detection of microbial species. These assays consist of up to four pooled unlabeled PCR primers and TaqMan probes with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Features of TaqMan Microbe Detection assays include:

  • Easy to use – just add DNA and master mix and run the qPCR – no melt curves required
  • Specific – TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
  • Sensitive – TaqMan assays are ideal for measuring low-abundance microbial targets
  • Accurate – identify microbial targets with high accuracy of quantitation
  • Extensive content – over 300 pre-designed microbe detection assays currently available
  • Gold-standard TaqMan qPCR chemistry – TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for closely related microbial species and subtypes
  • Fast delivery – assays shipped in as little as 1–2 days (see below)

Approximate ship time

  • Inventoried: 1–2 days in North America*
  • Made to order: 4–6 days in North America

All of our predesigned TaqMan gene expression assays are covered by the TaqMan Assays qPCR Guarantee.**

*Our most popular assays are stocked in North America for overnight delivery where possible. If an assay is out of stock, it may take an additional 3–5 business days for delivery.

**Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications
Modification de l’amorce de 3’FAM
Modification de l’amorce de 5’MGB-NFQ
FormatTube
Nbre de réactions250 réactions
Gamme de produitsTaqMan
Quantité250 μl
Conditions d’expéditionDry Ice
Concentration20X
Méthode de détectionAmorce-sonde
À utiliser avec (application)Pathogen Detection
Méthode PCRqPCR
Unit SizeEach
Contenu et stockage
Store at -15 to -25°C.

Foire aux questions (FAQ)

What are the concentrations of the primers and probes for TaqMan microbial assays?

Our predesigned and custom TaqMan microbial 20X assays have a primer concentration of 900 nM and a probe concentration of 250 nM in the final 1X concentration.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.