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Residual Protein TrypLE™ ELISA Kit
Gibco™

Residual Protein TrypLE™ ELISA Kit

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme.
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RéférenceQuantité
A65331025 kits, 480 tests
A6533101Kit de 96 tests
A653310310 kits, 960 tests
Référence A6533102
Prix (EUR)
-
Quantité:
5 kits, 480 tests

The Residual Protein TrypLE ELISA Kit is a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative detection of TrypLE enzyme. The kit is compatible with all forms of TrypLE enzyme, including TrypLE Select, TrypLE Express, and CTS TrypLE Select enzymes.

TrypLE enzyme is an animal-origin-free recombinant alternative to porcine trypsin. It is used for the dissociation of attachment-dependent cell lines. TrypLE enzyme dissociates cells cultured both in serum-free and serum-supplemented systems. Due to the unique biological sequence of TrypLE enzyme, traditional trypsin ELISA kits are ineffective at binding and detecting residual levels of TrypLE enzyme.

À des fins de recherche ou de commercialisation avancée Non destiné aux diagnostics ou à l’administration directe à des humains ou des animaux.

Spécifications
Plage de dosage0.78 to 50 ng/mL
Sensibilité du dosage0.032 ng/mL
ConjuguéHorseradish Peroxidase (HRP)
À utiliser avec (équipement)Lecteur de microplaques colorimétriques
FormatWash steps required
Coefficient de variation inter-dosage6.20%
Coefficient de variation intra-dosage7.20%
Marqueur ou colorantHorseradish Peroxidase (HRP)
Alias de protéinesTrypLE, R-Protease
Quantité5 kits, 480 tests
Conditions d’expéditionGlace
CibleTrypLE (R-Protease) concentration
Durée totale du dosage1.5 hr.
Format du kit d’immunoessaiKit ELISA sandwich
Unit SizeEach
Contenu et stockage
• Pre-coated 96-well plate
• Standard assay buffer concentrate
• HRP-conjugated detection antibody
• Wash buffer concentrate
• Chromogen stop solution
• Adhesive plate covers

Store at 2–8°C.

Foire aux questions (FAQ)

How can I assess residual TrypLE reagent remaining after my downstream processing steps?

We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.