POROS™ CaptureSelect™ C1-InhXL Affinity Resin

POROS CaptureSelect C1-InhXL Affinity Resin is intended for the purification of human C1 esterase inhibitor from recombinant and plasma/serum sources. C1 esterase inhibitor is a protease inhibitor belonging to the serpin superfamily that inhibits the spontaneous activation of the complement system. The CaptureSelect C1-InhXL affinity ligand is highly selective for human C1 inhibitor and coupled to the high-performing POROS resin, allowing for an efficient and scalable purification process, with high purity and yield obtained in a single step. The large pore structure of the POROS beads enables higher flowrates and efficient binding of larger biomolecules.

Features of POROS CaptureSelect C1-InhXL Affinity Resin include:

• Efficient purification of C1 esterase inhibitor with high yield
• Mild pH elution condition to retain activity
• High binding capacity at high flow rates
• Excellent scalability
• Non-animal derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

• Resin: cross-linked poly(styrene-divinylbenzene) POROS beads
• Average particle size: 50 μm
• Ligand: single-domain [VHH] antibody fragment
• Binding capacity: 20–25 g C1-Inhibitor/L resin
• Elution conditions: 50 mM sodium acetate, 1.0 M MgCl₂, pH 5.0
• Regeneration conditions: acidic stripping condition with 0.5 M acetic acid, pH 2
• Mechanical resistance: 100 bar (1450 psi, 10 MPa)
• Formulation buffer: 18% (v/v) ethanol