DiSBAC<sub>2</sub>(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)

Citations et références (120)

Invitrogen™

DiSBAC₂(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)

La sonde sensible au potentiel de réponse lente , DiSBAC2(3) peut pénétrer dans les cellules dépolarisées où elle se lieAfficher plus

Référence	Quantité
B413	100 mg

Référence B413

Prix (EUR)

536,00

Quantité:

100 mg

Prix (EUR)

536,00

La sonde sensible au potentiel de réponse lente , DiSBAC₂(3) peut pénétrer dans les cellules dépolarisées où elle se lie aux protéines intracellulaires ou à la membrane et présente une fluorescence accrue et un décalage spectral vers le rouge. Une dépolarisation accrue entraîne un afflux supplémentaire de colorant anionique et une augmentation de la fluorescence. Inversement, l’hyperpolarisation est indiquée par une diminution de la fluorescence. Les changements de fluorescence dépendant des potentiels générés par ce bis-oxonol sont généralement ∼1 % par mV. En outre, cette sonde possède des maxima d’excitation de 530 nm et des maxima d’émission de 560 nm. Les colorants DiBAC sont exclus des mitochondries en raison de leur charge négative globale, ce qui les rend supérieurs aux carbocyanes pour mesurer les potentiels de membrane plasmique.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Type de colorantSondes de potentiel de membrane

Quantité100 mg

Conditions d’expéditionTempérature ambiante

Type de produitDiSBAC2(3)

Unit SizeEach

Contenu et stockage

Stocker à température ambiante et à l’abri de la lumière.

Foire aux questions (FAQ)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (120)

Citations et références

Abstract

Use of a membrane potential-sensitive probe to assess biological expression of the cystic fibrosis transmembrane conductance regulator.

Authors:Renier M, Tamanini A, Nicolis E, Rolfini R, Imler JL, Pavirani A, Cabrini G

Journal:Hum Gene Ther

PubMed ID:8590731

Cystic fibrosis is caused by defects in a chloride-transporting protein termed cystic fibrosis transmembrane conductance regulator (CFTR). This study presents an innovative procedure to evaluate expression of functional CFTR. The technique uses the potential-sensitive probe bis-(1,3-diethylthiobarbituric acid) trimethine oxonol or DiSBAC2(3), by single-cell fluorescence imaging. The DiSBAC2(3) method was first ... More

Expression of the voltage-gated sodium channel NaV1.5 in the macrophage late endosome regulates endosomal acidification.

Authors:Carrithers MD, Dib-Hajj S, Carrithers LM, Tokmoulina G, Pypaert M, Jonas EA, Waxman SG,

Journal:J Immunol

PubMed ID:17548620

'Voltage-gated sodium channels expressed on the plasma membrane activate rapidly in response to changes in membrane potential in cells with excitable membranes such as muscle and neurons. Macrophages also require rapid signaling mechanisms as the first line of defense against invasion by microorganisms. In this study, our goal was to ... More

Heat shock- and ethanol-induced ionic changes in C6 rat glioma cells determined by NMR and fluorescence spectroscopy.

Authors:Skrandies S, Bremer B, Pilatus U, Mayer A, Neuhaus-Steinmetz U, Rensing L

Journal:Brain Res

PubMed ID:9037501

'The effects of two different stressors, heat shock (HS; 44 degrees C, 20 min) and ethanol (1.2 M, 60 min), on ion content and membrane potential were investigated in C6 rat glioma cells. Both treatments were previously shown to induce the HS response [26]. Intracellular pH (pH(i)), sodium ion concentration ... More

Membrane potential modulates release of tumor necrosis factor in lipopolysaccharide-stimulated mouse macrophages.

Authors:Haslberger A, Romanin C, Koerber R

Journal:Mol Biol Cell

PubMed ID:1498365

'Lipopolysaccharide (LPS)-mediated synthesis of macrophage gene products such as tumor necrosis factor (TNF) is controlled by different signaling pathways. We investigated intracellular free Ca2+ (Ca2+ic) and the membrane potential as early cellular responses to LPS and their role in the synthesis and release of TNF. In peritoneal macrophages and in ... More

Selective modulation by cGMP of the K+ channel activated by speract.

Authors:Cook SP, Babcock DF

Journal:J Biol Chem

PubMed ID:7693667

'The egg peptide speract stimulates sperm guanylyl cyclase and presumably enhances fertilization, but the roles of cGMP in sperm responses are yet undetermined. Here we show that speract-induced accumulation of cGMP or cAMP is selectively enhanced by the phosphodiesterase inhibitors, 3-isobutyl-1-methylxanthine (IBMX) or papaverine, respectively. These inhibitors provided the unusual ... More

View all DiSBAC₂(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol) citations

DiSBAC2(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)

Foire aux questions (FAQ)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

What is the difference between fast and slow-response membrane potential probes?

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

Citations et références (120)

DiSBAC₂(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)