Human Epidermal Keratinocytes, neonatal (HEKn)
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Gibco™

Human Epidermal Keratinocytes, neonatal (HEKn)

Les kératinocytes épidermiques humains, nouveau-nés primaires normaux (HEKn) sont isolés du prépuce néonatal et cryoconservés à la fin de laAfficher plus
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C0015C1 flacon
Référence C0015C
Prix (EUR)
912,00
Each
Ajouter au panier
Quantité:
1 flacon
Prix (EUR)
912,00
Each
Ajouter au panier
Les kératinocytes épidermiques humains, nouveau-nés primaires normaux (HEKn) sont isolés du prépuce néonatal et cryoconservés à la fin de la culture primaire.

Les cellules sont :
• Testées quant à leurs performances—au moins 30 doublages de population en culture dans un milieu EpiLife complété par du HKGS (pour HEKn) ou le supplément S7 (pour HEKn-AOF)
• Garanties pour être ≥ 70 % viables
• Testées quant aux mycoplasmes, bactéries, levures ou autres champignons, hépatite B, hépatite C, et virus VIH-1

Chaque flacon contient plus de 0,5 x 106 cellules viables qui ont été cryoconservées à la fin du stade de culture primaire dans un milieu contenant 10 % de DMSO.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Source du donneurDonneur unique
Nbre de cellules≥ 500000
Gamme de produitsProduits biologiques en cascade
Type de produitKératinocyte épidermique humain
Quantité1 flacon
Testé pourHépatite B (VHB), hépatite C (VHC), tests de stérilité (bactéries et champignons), mycoplasmes, test de performance de croissance, VIH-1
AgeNouveau-né (14 jours ou moins)
Type de celluleCellules kératinocytes
Culture TypeCulture de cellules adhérentes
FormeCryoconservé
EspècesHumain
Unit SizeEach
Contenu et stockage
Stocké dans l’azote liquide.

Foire aux questions (FAQ)

I see large, flat pancake-looking cells in my keratinocyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care an attention, the culture should yield at least 25 population doublings.

I see large, flat pancake-looking cells in my Human Keratinodyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size, should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care and attention, the culture should yield at least 25 population doublings.

What is the doubling time for Human Keratinodyte?

Each lot is tested for doubling time and the results are listed on the COA.

Can I specify a donor type for the primary cells you offer?

This information also appears on the COA.

What is the difference between basal cells or basal keratinocytes?

These are all the same. Sometimes Keratinocytes are referred to as basal cells or basal keratinocytes.

View all Human Epidermal Keratinocytes, neonatal (HEKn) FAQs

Citations et références (1)

Citations et références
Abstract
High Sp1/Sp3 ratios in epithelial cells during epithelial differentiation and cellular transformation correlate with the activation of the HPV-16 promoter.
Authors:Apt D, Watts RM, Suske G, Bernard HU,
Journal:Virology
PubMed ID:8862423
Gene expression of human papillomavirus type 16 (HPV-16) and other HPV types is epithelial specific. Specificity is brought about by synergism between several different transcription factors that seem to occur ubiquitously but differ qualitatively and quantitatively between cells in which HPV genomes are transcriptionally active or inactive. Here, we report ... More