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Citations et références (382)
Invitrogen™
6-FAM (6-Carboxyfluorescein), single isomer
6-FAM (6-carboxyfluorescéine), isomère simple
| Référence | Quantité |
|---|---|
| C1360 | 100 mg |
Référence C1360
Prix (EUR)
325,00
Each
Quantité:
100 mg
Prix (EUR)
325,00
Each
L’isomère simple, 6-FAM, contient un acide carboxylique qui peut être utilisé pour réagir avec les amines primaires via l’activation carbodiimide de l’acide carboxylique. La fluorescéine est le réactif de dérivatisation fluorescent le plus courant pour le marquage des biomolécules. Outre son absorptivité relativement élevée, son excellent rendement quantique de fluorescence et sa bonne solubilité dans l’eau, la fluorescéine possède une excitation maximale qui correspond étroitement à la ligne spectrale de 488 nm du laser argon-ion.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Réactivité chimiqueAmine
Excitation488
Marqueur ou colorantFITC (fluorescéine)
Type de produit6-FAM
Quantité100 mg
Groupement de réactifsAcide carboxylique
Conditions d’expéditionTempérature ambiante
Type d’étiquetteColorants classiques
Unit SizeEach
Contenu et stockage
Stocker à température ambiante et à l’abri de la lumière.
Citations et références (382)
Citations et références
Abstract
Membrane vesicles containing the Sendai virus binding glycoprotein, but not the viral fusion protein, fuse with phosphatidylserine liposomes at low pH.
Journal:Biochemistry
PubMed ID:3021204
Membrane vesicles containing the Sendai virus hemagglutinin/neuraminidase (HN) glycoprotein were able to induce carboxyfluorescein (CF) release from loaded phosphatidylserine (PS) but not loaded phosphatidylcholine (PC) liposomes. Similarly, fluorescence dequenching was observed only when HN vesicles, bearing self-quenched N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylethanolamine (N-NBD-PE), were incubated with PS but not PC liposomes. Thus, fusion between
Receptor-mediated endocytosis of antibody-opsonized liposomes by tumor cells.
Journal:Proc Natl Acad Sci U S A
PubMed ID:7001454
'Specific receptor-mediated delivery of the contents of small, sonicated liposomes was studied with three murine tumor cell types: an IgG Fc receptor-negative nonphagocytic line (EL4); an Fc receptor-positive phagocytic line (P388D1); and an Fc receptor-positive nonphagocytic line (P388). The liposomes (formed from phosphatidylcholines, cholesterol, and dinitrophenyl-substituted phosphatidylethanolamine) contained carboxyfluorescein as
Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein.
Journal:Biochemistry
PubMed ID:1510932
'Water rapidly crosses the plasma membranes of red blood cells (RBCs) and renal tubules through highly specialized channels. CHIP28 is an abundant integral membrane protein in RBCs and renal tubules, and Xenopus laevis oocytes injected with CHIP28 RNA exhibit high osmotic water permeability, Pf [Preston et al. (1992) Science 256,
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent
Luminal pH regulated calcium release kinetics in sarcoplasmic reticulum vesicles.
Journal:Biochemistry
PubMed ID:8873610
'Calcium binding to triads isolated from rabbit skeletal muscle followed a single hyperbolic function in the pH range 5.5-8.0. Maximal binding was obtained at pH 8.0; decreasing the pH decreased the binding capacity and, at pH < or = 6.0, increased Kd 2-fold. These results indicate that lowering the pH