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Citations et références (238)
Invitrogen™
5-TAMRA, SE (5-carboxytétraméthylrhodamine, ester de succinimidyle), isomère simple
Le 5-TAMRA réactif aux amines, SE et ses conjugués produisent une fluorescence rouge-orange brillante et insensible au pH (maxima d’excitationAfficher plus
| Référence | Quantité |
|---|---|
| C2211 | 5 mg |
Référence C2211
Prix (EUR)
534,65
Online Exclusive
712,00Économisez 177,35 (25%)
Each
Quantité:
5 mg
Prix (EUR)
534,65
Online Exclusive
712,00Économisez 177,35 (25%)
Each
Le 5-TAMRA réactif aux amines, SE et ses conjugués produisent une fluorescence rouge-orange brillante et insensible au pH (maxima d’excitation / émission d’environ ∼ 546 / 579) avec une bonne photostabilité.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Réactivité chimiqueAmine
Émission579
Excitation546
Marqueur ou colorantIsomères TAMRA™, TMR (tétraméthylrhodamine)
Type de produit5-TAMRA SE
Quantité5 mg
Groupement de réactifsEster actif, ester de succinimidyle
Conditions d’expéditionTempérature ambiante
Type d’étiquetteColorants classiques
Unit SizeEach
Contenu et stockage
Conserver au congélateur (-5 à -30°C) à l’abri de la lumière.
Citations et références (238)
Citations et références
Abstract
Multiplex detection of single-nucleotide variations using molecular beacons.
Journal:Genetic analysis : biomolecular engineering
PubMed ID:10084107
Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification.
Journal:Clin Chem
PubMed ID:9166227
We describe a PCR-based assay for determining c-erbB-2 oncogene amplification in breast cancer in which we use the TaqMan system. Two fluorogenic probes anneal to the target between primers for c-erbB-2 and beta-globin genes and contain both a reporter dye (6-carboxy-fluorescein) and a quencher dye (6-carboxy-tetramethyl-rhodamine). During the extension phase
DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.
Journal:Nucleic Acids Res
PubMed ID:1598205
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments which were made single-stranded by digestion with T7 gene 6
Fluorescence resonance energy transfer analysis of the structure of the four-way DNA junction.
Journal:Biochemistry
PubMed ID:1591245
We have carried out fluorescence resonance energy transfer (FRET) measurements on four-way DNA junctions in order to analyze the global structure and its dependence on the concentration of several types of ions. A knowledge of the structure and its sensitivity to the solution environment is important for a full understanding
A novel fluorescent toxin to detect and investigate Kv1.3 channel up-regulation in chronically activated T lymphocytes.
Journal:J Biol Chem
PubMed ID:12511563
T lymphocytes with unusually high expression of the voltage-gated Kv1.3 channel (Kv1.3(high) cells) have been implicated in the pathogenesis of experimental autoimmune encephalomyelitis, an animal model for multiple sclerosis. We have developed a fluoresceinated analog of ShK (ShK-F6CA), the most potent known inhibitor of Kv1.3, for detection of Kv1.3(high) cells