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Sondes fluorescentes CellTracker™
Citations et références (207)
Invitrogen™

Sondes fluorescentes CellTracker™

Colorant fluorescent bien adapté pour la surveillance du mouvement ou de la localisation cellulaire
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RéférenceQuantitéType de colorant
C29251mgCellTracker Vert CMFDA
C21105 mgCellTracker™ Bleu CMAC
C21115 mgCellTracker™ Bleu CMHC
C128815 mgCellTracker bleu CMF2HC
C3456520 x 15 μgAutre(s) étiquette(s) ou colorant(s)
C702520 x 50 μgCellTracker Vert CMFDA
C21025 mgColorants BODIPY
C3455120 x 50 μgCellTracker orange CMRA
C29271 mgCellTracker Orange CMTMR
C3455220 x 50 μgCellTracker™ Rouge CMTPX
C100945 x 0,1 mgThiolTracker™ Violet
Référence C2925
Prix (EUR)
573,65
Precio exclusivo en nuestra web
610,00
Économisez 36,35 (6%)
Each
Quantité:
1mg
Type de colorant:
CellTracker Vert CMFDA
Prix (EUR)
573,65
Precio exclusivo en nuestra web
610,00
Économisez 36,35 (6%)
Each
Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

  • Ce colorant est bien retenu, ce qui permet le suivi multigénérationnel des mouvements cellulaires.
  • Les spectres d’excitation / émission verts (492/517 nm maximum) sont optimaux pour le multiplexage avec des colorants fluorescents rouges et des protéines
  • Facile à utiliser: enlevez le milieu, ajoutez le colorant, laissez incuber pendant 30 minutes et imagez les cellules
  • Rétention du signal fluorescent de >72 heures (typiquement de trois à six générations)
  • Faible cytotoxicité : n’affecte pas la viabilité ni la prolifération.
  • Conçu pour traverser librement les membranes cellulaires jusqu’aux cellules, où ils sont transformés en produits de réaction imperméable aux membranes cellulaires.
  • Le colorant est transféré aux cellules filles mais pas aux cellules adjacentes dans une population.
  • Stable, non toxique aux concentrations de fonctionnement, bien retenu par les cellules et fluorescence très lumineuse à un pH physiologique

Adhérence cellulaire, analyse cellulaire, prolifération cellulaire, traçage et suivi des cellules, viabilité cellulaire et cytotoxicité, viabilité cellulaire, prolifération et fonction, imagerie cellulaire, tests de toxicologie cellulaire, chimiotaxie et migration cellulaire, détection et développement de médicaments, traçage cellulaire général, détection de glutathion, analyse à haute densité (HCS), immunofluorescence (IF), coloration et détection de cellules par immunofluorescence, homéostasie et signalisation ionique, suivi microbien, stress nitro-oxydatif, tests ADME/Tox basés sur la cible, détection du pH.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
CouleurGreen
DescriptionCellTracker™ Green CMFDA Dye, 1 mg
Type de colorantCellTracker Vert CMFDA
Émission517 nm
Gamme de longueur d’onde d’excitation492 nm
FormeDry Powder
Gamme de produitsCellTracker
Quantité1mg
Type de réactifComposés de suivi cellulaire, réactifs de marquage cellulaire
Conditions d’expéditionTempérature ambiante, Température ambiante
Type d’étiquetteAutres marqueurs ou colorants
Type de produitColorant
SubCellular LocalizationCytoplasm
Unit SizeEach
Contenu et stockage
Conserver au congélateur (-5 et -30°C).

Foire aux questions (FAQ)

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is DAPI a good live-cell nuclear label?

DAPI is considered a semi-permeant/impermeant nucleic acid stain. Staining of nucleic is dependent upon the cell line in its performance. Some cell lines will label with DAPI, others not at all, and others label inconsistenly. Instead, we recommend using either Hoechst 33342 or Hoechst 33258, which have the same wavelength and binding mode as DAPI (at the A-T minor groove) but are readily cell-permeant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the nuclei of live cells and track them over time. Can I use DAPI for this?

We do not recommend doing this. DAPI is considered to be a semi-permeant/impermeant nucleic acid stain. DAPI staining of live cells may be inconsistent. It is best used as a counterstain for fixed samples. Other cell permeable nucleic acid stains, such as Hoechst or the SYTO dyes may affect cellular function.

For mammalian cells, we recommend using the CellLight Nucleus transduction reagents, available in CFP, GFP and RFP. With these reagents, the cells are transduced overnight in a single labeling step and the next day the nuclei will fluoresce. The label may be retained for 3-5 days and should not affect cell function. Cytoplasmic cell tracking dyes such as the CellTracker dyes may also be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the CellTracker dyes be fixed?

Yes, the CellTracker dyes react with any accessible thiol part of the protein and can be fixed. However, some CellTracker dyes may be attached to small metabolites that can leak from the cell following permeabilization. This can result in decreased fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

View all Sondes fluorescentes CellTracker™ FAQs

Citations et références (207)

Citations et références
Abstract
In situ image analysis of interactions between normal human keratinocytes and fibroblasts cultured in three-dimensional fibrin gels.
Authors:Sun T,Haycock J,Macneil S
Journal:Biomaterials
PubMed ID:16510181
A point mutation in the binding subunit of a retroviral envelope protein arrests virus entry at hemifusion.
Authors:Zavorotinskaya T, Qian Z, Franks J, Albritton LM
Journal:J Virol
PubMed ID:14671127
The transmembrane subunits of viral envelope proteins are thought to perform all of the functions required for membrane fusion during entry of enveloped viruses. However, changes in a conserved SPHQ motif near the N terminus of the receptor binding subunit of a murine leukemia virus (MLV) envelope protein block infection ... More
Intercellular spreading of Porphyromonas gingivalis infection in primary gingival epithelial cells.
Authors:Yilmaz O, Verbeke P, Lamont RJ, Ojcius DM
Journal:Infect Immun
PubMed ID:16369027
'Porphyromonas gingivalis, an important periodontal pathogen, is an effective colonizer of oral tissues. The organism successfully invades, multiplies in, and survives for extended periods in primary gingival epithelial cells (GECs). It is unknown whether P. gingivalis resides in the cytoplasm of infected cells throughout the infection or can spread to ... More
Increased apical insertion of the multidrug resistance protein 2 (MRP2/ABCC2) in renal proximal tubules following gentamicin exposure.
Authors:Notenboom S, Wouterse AC, Peters B, Kuik LH, Heemskerk S, Russel FG, Masereeuw R,
Journal:J Pharmacol Exp Ther
PubMed ID:16757538
'Multidrug resistance protein (MRP) 2 (MRP2; ABCC2), an organic anion transporter apically expressed in liver, kidney, and intestine, plays an important protective role through facilitating the efflux of potentially toxic compounds. We hypothesized that upon a toxic insult, MRP2 is up-regulated in mammalian kidney, thereby protecting the tissue from damage. ... More
Apoptosis of adherent cells by recruitment of caspase-8 to unligated integrins.
Authors:Stupack DG, Puente XS, Boutsaboualoy S, Storgard CM, Cheresh DA
Journal:J Cell Biol
PubMed ID:11684710
'Integrin-mediated adhesion promotes cell survival in vitro, whereas integrin antagonists induce apoptosis of adherent cells in vivo. Here, we demonstrate that cells adherent within a three-dimensional extracellular matrix undergo apoptosis due to expression of unligated integrins, the beta subunit cytoplasmic domain, or its membrane proximal sequence KLLITIHDRKEF. Integrin-mediated death requires ... More
View all Sondes fluorescentes CellTracker™ citations