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Calcéine AM, colorants vert et bleu capables de pénétrer dans les cellules
Citations et références (662)
Invitrogen™

Calcéine AM, colorants vert et bleu capables de pénétrer dans les cellules

La calcéine AM est un colorant cellulaire perméant qui peut être utilisé pour déterminer la viabilité cellulaire de la plupartAfficher plus
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RéférenceType de produitQuantitéCouleur
C3100MPCalcéine, AM20 x 50 μgGreen
C1430Calcéine, AM1 mgGreen
C3099Calcéine AM (solution à 1 mg/ml dans du DMSO sec)1 mLGreen
C1429Calcéine bleue, AM1 mgBlue
C34852Calcéine verte, AM20 x 50 μgGreen
C481Calcéine, haute pureté100 mgGreen
Référence C3100MP
Prix (EUR)
631,65
キャンペーン価格
694,00
Économisez 62,35 (9%)
Each
Type de produit:
Calcéine, AM
Quantité:
20 x 50 μg
Couleur:
Green
Prix (EUR)
631,65
キャンペーン価格
694,00
Économisez 62,35 (9%)
Each
La calcéine AM est un colorant cellulaire perméant qui peut être utilisé pour déterminer la viabilité cellulaire de la plupart des cellules eucaryotes. Dans les cellules vivantes, la calcéine AM non fluorescente est convertie en calcéine à fluorescence verte, après hydrolyse d’ester acétoxyméthylique par estérases intracellulaires. Ce colorant est également disponible sous forme solide à raison de 1 mg (C-1430) à remettre en suspension dans du DMSO (C-3099). Pour une version à plus grande longueur d’onde de ce colorant, testez notre nouvelle calcéine AM rouge-orangée CellTrace (C-34851).
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Perméabilité cellulairePerméable aux cellules
Type de colorantAutre(s) étiquette(s) ou colorant(s)
Poids moléculaire994.86
Quantité20 x 50 μg
Type de réactifComposés de suivi cellulaire, réactifs de marquage cellulaire
Conditions d’expéditionTempérature ambiante, Température ambiante
Enzyme cibleEstérase
CouleurGreen
Emission517 nm
À utiliser avec (application)Traçage cellulaire, Traceur cellulaire
À utiliser avec (équipement)Microscope à fluorescence
Type de produitCalcéine, AM
Unit SizeEach
Contenu et stockage
Conserver au congélateur (-5 et -30°C).

Foire aux questions (FAQ)

I would like a dye to load in live cells such that it will self-quench at a high concentration, but if the cell dies, the dye will be released and unquenched. Do you have anything like that?

Yes. This is commonly done with calcein AM or FDA (fluorescein diacetate). These dyes will not fluoresce until cleaved by esterases. After modification by esterases and at very high concentrations, they will self-quench. Upon disruption of the plasma membrane, or cell death, the dye will be released into the extracellular medium, and become unquenched. Concentration and incubation time must be optimized to obtain adequate quenching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to load liposomes with calcein. Should I use the AM form or the non-AM form?

Calcein, AM requires esterase cleavage of the acetoxymethyl (AM) ester to become fluorescent. Liposomes don't have esterases unless specifically constructed to include the enzyme. The water-soluble, non-AM form of calcein (Cat. No. C481), does not require esterase cleavage to be fluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?

This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a general cytoplasmic stain that does not overlap with the GFP in my cells. What do you recommend?

Calcein AM, a green dye, is typically used as a general cytoplasmic stain, but not recommended with GFP-positive cells. For GFP-expressing cells there are other colors available: Calcein Blue AM, Calcein Violet AM, and Calcein Red-Orange AM. The retention time of these dyes in live cells is dependent upon the inherent properties of the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Calcéine AM, colorants vert et bleu capables de pénétrer dans les cellules FAQs

Citations et références (662)

Citations et références
Abstract
Nitric oxide mediates natural polyphenol-induced Bcl-2 down-regulation and activation of cell death in metastatic B16 melanoma.
Authors:Ferrer P,Asensi M,Priego S,Benlloch M,Mena S,Ortega A,Obrador E,Esteve JM,Estrela JM
Journal:The Journal of biological chemistry
PubMed ID:17135264
Cloning and expression of murine sister of P-glycoprotein reveals a more discriminating transporter than MDR1/P-glycoprotein.
Authors:Lecureur V,Sun D,Hargrove P,Schuetz EG,Kim RB,Lan LB,Schuetz JD
Journal:Molecular pharmacology
PubMed ID:10617675
Deposition of laminin 5 by keratinocytes regulates integrin adhesion and signaling.
Authors:Nguyen BP,Gil SG,Carter WG
Journal:The Journal of biological chemistry
PubMed ID:10926936
Large-scale chemical dissection of mitochondrial function.
Authors:Wagner BK,Kitami T,Gilbert TJ,Peck D,Ramanathan A,Schreiber SL,Golub TR,Mootha VK
Journal:Nature biotechnology
PubMed ID:18297058
Mitochondrial oxidative phosphorylation (OXPHOS) is central to physiology and disease pathogenesis. To systematically investigate its activity and regulation, we performed a wide range of assays of OXPHOS physiology and nuclear and mitochondrial gene expression across 2490 chemical perturbations in muscle cells. Through mining of the resulting compendium, we discovered that: ... More
Modification of the cytoplasmic domain of influenza virus hemagglutinin affects enlargement of the fusion pore.
Authors:Kozerski C,Ponimaskin E,Schroth-Diez B,Schmidt MF,Herrmann A
Journal:Journal of virology
PubMed ID:10906206
The fusion activity of chimeras of influenza virus hemagglutinin (HA) (from A/fpv/Rostock/34; subtype H7) with the transmembrane domain (TM) and/or cytoplasmic tail (CT) either from the nonviral, nonfusogenic T-cell surface protein CD4 or from the fusogenic Sendai virus F-protein was studied. Wild-type or chimeric HA was expressed in CV-1 cells ... More
View all Calcéine AM, colorants vert et bleu capables de pénétrer dans les cellules citations