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Sondes fluorescentes CellTracker™
Citations et références (37)
Invitrogen™

Sondes fluorescentes CellTracker™

Colorant fluorescent bien adapté pour la surveillance du mouvement ou de la localisation cellulaire
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RéférenceQuantitéType de colorant
C3455120 x 50 μgCellTracker orange CMRA
C21105 mgCellTracker™ Bleu CMAC
C21115 mgCellTracker™ Bleu CMHC
C128815 mgCellTracker bleu CMF2HC
C3456520 x 15 μgAutre(s) étiquette(s) ou colorant(s)
C702520 x 50 μgCellTracker Vert CMFDA
C29251mgCellTracker Vert CMFDA
C21025 mgColorants BODIPY
C29271 mgCellTracker Orange CMTMR
C3455220 x 50 μgCellTracker™ Rouge CMTPX
C100945 x 0,1 mgThiolTracker™ Violet
Référence C34551
Prix (EUR)
729,65
線上優惠
776,00
Économisez 46,35 (6%)
Each
Quantité:
20 x 50 μg
Type de colorant:
CellTracker orange CMRA
Prix (EUR)
729,65
線上優惠
776,00
Économisez 46,35 (6%)
Each
Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.

  • Ce colorant est bien retenu, ce qui permet le suivi multigénérationnel des mouvements cellulaires.
  • Les spectres d’excitation / émission verts (492/517 nm maximum) sont optimaux pour le multiplexage avec des colorants fluorescents rouges et des protéines
  • Facile à utiliser: enlevez le milieu, ajoutez le colorant, laissez incuber pendant 30 minutes et imagez les cellules
  • Rétention du signal fluorescent de >72 heures (typiquement de trois à six générations)
  • Faible cytotoxicité : n’affecte pas la viabilité ni la prolifération.
  • Conçu pour traverser librement les membranes cellulaires jusqu’aux cellules, où ils sont transformés en produits de réaction imperméable aux membranes cellulaires.
  • Le colorant est transféré aux cellules filles mais pas aux cellules adjacentes dans une population.
  • Stable, non toxique aux concentrations de fonctionnement, bien retenu par les cellules et fluorescence très lumineuse à un pH physiologique

Adhérence cellulaire, analyse cellulaire, prolifération cellulaire, traçage et suivi des cellules, viabilité cellulaire et cytotoxicité, viabilité cellulaire, prolifération et fonction, imagerie cellulaire, tests de toxicologie cellulaire, chimiotaxie et migration cellulaire, détection et développement de médicaments, traçage cellulaire général, détection de glutathion, analyse à haute densité (HCS), immunofluorescence (IF), coloration et détection de cellules par immunofluorescence, homéostasie et signalisation ionique, suivi microbien, stress nitro-oxydatif, tests ADME/Tox basés sur la cible, détection du pH.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
CouleurOrange
DescriptionCellTracker™ Orange CMRA Dye, 20 x 50 μg
Type de colorantCellTracker orange CMRA
Émission576
Gamme de longueur d’onde d’excitation548 / 576
FormeDry Powder
Gamme de produitsCellTracker
Quantité20 x 50 μg
Type de réactifComposés de suivi cellulaire, réactifs de marquage cellulaire
Conditions d’expéditionTempérature ambiante, Température ambiante
Type d’étiquetteAutres marqueurs ou colorants
Type de produitColorant
SubCellular LocalizationCytoplasm
Unit SizeEach
Contenu et stockage
Conservez au congélateur (entre -5 et -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the CellTracker dyes be fixed?

Yes, the CellTracker dyes react with any accessible thiol part of the protein and can be fixed. However, some CellTracker dyes may be attached to small metabolites that can leak from the cell following permeabilization. This can result in decreased fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to stain my cells with CellTracker dyes or CFDA SE, but I'm not seeing much signal. What can I do?

First, make sure you aren’t staining in the presence of serum, since serum can have esterase activity that can prematurely cleave the AM group on these dyes, preventing entry into cells. After staining, it’s okay to return the cells to medium containing serum. After this, you can try increasing the concentration and label time to get a higher intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Sondes fluorescentes CellTracker™ FAQs

Citations et références (37)

Citations et références
Abstract
Liver-specific functional studies in a microfluidic array of primary mammalian hepatocytes.
Authors:Kane BJ, Zinner MJ, Yarmush ML, Toner M
Journal:Anal Chem
PubMed ID:16808435
'Nearly half a billion dollars in resources are lost each time a drug candidate is withdrawn from the market by the Food and Drug Administration (FDA) for reasons of liver toxicity. The number of late-phase drug developmental failures due to liver toxicity could potentially be reduced through the use of ... More
Vascular endothelial growth factor-C and C-C chemokine receptor 7 in tumor cell-lymphatic cross-talk promote invasive phenotype.
Authors:Issa A, Le TX, Shoushtari AN, Shields JD, Swartz MA,
Journal:Cancer Res
PubMed ID:19118020
'Most carcinomas spread to distant sites through lymphatic vessels. Several preclinical and clinical studies have shown a positive correlation between the incidence of lymph node metastasis and secretion of the lymphatic growth factor vascular endothelial growth factor-C (VEGF-C) by tumor cells, suggesting tumor lymphangiogenesis as an escape mechanism. However, recent ... More
Editing antigen presentation: antigen transfer between human B lymphocytes and macrophages mediated by class A scavenger receptors.
Authors:Harvey BP, Quan TE, Rudenga BJ, Roman RM, Craft J, Mamula MJ,
Journal:J Immunol
PubMed ID:18768860
'B lymphocytes can function independently as efficient APCs. However, our previous studies demonstrate that both dendritic cells and macrophages are necessary to propagate immune responses initiated by B cell APCs. This finding led us to identify a process in mice whereby Ag-specific B cells transfer Ag to other APCs. In ... More
Quantitative 3D video microscopy of HIV transfer across T cell virological synapses.
Authors:Hübner W, McNerney GP, Chen P, Dale BM, Gordon RE, Chuang FY, Li XD, Asmuth DM, Huser T, Chen BK,
Journal:Science
PubMed ID:19325119
The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV ... More
Coculture methodologies for the study of Wnt signals.
Authors:Planutis K, Planutiene M, Holcombe RF,
Journal:Methods Mol Biol
PubMed ID:19099261
In vivo, responses to extracellular Wnt ligands are context dependent; the temporal characteristics and intensity of the signal are critical in determining the target cell response. In general, Wnt ligand-induced differentiation in mammalian cells requires several days of exposure. In order to better characterize Wnt-induced signaling in vitro, side-by-side and ... More
View all Sondes fluorescentes CellTracker™ citations