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Test de prolifération cellulaire CyQUANT™ NF
Citations et références (65)
Invitrogen™

Test de prolifération cellulaire CyQUANT™ NF

Le test de prolifération cellulaire CyQUANT™ NF offre une méthode rapide et sensible pour compter les cellules dans une populationAfficher plus
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RéférenceType de celluleQuantité
C7026For cells in culture1000 analyses
C35011Direct Cell10 Microplates
C35013Direct Red Cell10 Microplaques
C35012Direct Cell100 Microplates
C35007NF Cell200 Assays
C35006NF Cell1000 Assays
C7027Cell Lysis Buffer50 ml
Référence C7026
Prix (EUR)
722,00
Each
Type de cellule:
For cells in culture
Quantité:
1000 analyses
Prix (EUR)
722,00
Each
Le test de prolifération cellulaire CyQUANT™ NF offre une méthode rapide et sensible pour compter les cellules dans une population et mesurer la prolifération au format microplaque.

Caractéristiques du test de prolifération cellulaire CyQUANT™ NF :

• Plus sensible que les tests MTT ou™ AlamarBlue
• Plage de détection linéaire de 100 à 20 000 cellules par puits (microplaque de 96 puits)
• Les analyses peuvent être effectuées en une heure

Une méthode simple et rapide pour mesurer la prolifération cellulaire
Le test de prolifération cellulaire CyQUANT™ NF ne nécessite pas de lyse cellulaire, de longues incubations, de radioactivité ou d’élimination du colorant des cellules. Le dosage CyQUANT™ NF élimine l’étape de lyse cellulaire par congélation-décongélation du dosage de prolifération cellulaire CyQUANT™ d’origine grâce à l’utilisation d’un colorant de liaison d’ADN perméant aux cellules en combinaison avec un réactif de perméabilisation de membrane plasmique. Le test de prolifération cellulaire CyQUANT™ NF peut être utilisé dans des formats de microplaques de 96 puits ou 384 puits, et est disponible en deux configurations un kit de 200 tests (C35007) pour les échantillons de plus petite taille et un kit de 1 000 tests (C35006) pour les applications à haut débit.

AlamarBlue™ est une marque déposée de TREK Diagnostic Systems
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Perméabilité cellulaireImperméant aux cellules
Type de celluleFor cells in culture
DescriptionTest de prolifération cellulaire CyQUANT™, pour cellules en culture
Méthode de détectionFluorescence
Type de colorantAutre(s) étiquette(s) ou colorant(s)
Excitation / émission480/520
FormatPlaque 96 puits
Quantité1000 analyses
Conditions d’expéditionTempérature ambiante
CouleurVert
Emission520 nm
Excitation Wavelength Range480 nm
À utiliser avec (application)Test de prolifération
À utiliser avec (équipement)Lecteur de microplaques
Gamme de produitsCyQUANT
Type de produitTest de prolifération cellulaire
Unit SizeEach
Contenu et stockage
Contient du colorant CyQUANT™ GR, le tampon de lyse cellulaire et l’ADN λ de bactériophage.

Foire aux questions (FAQ)

Can CyQUANT Cell Proliferation Assay, for cells in culture (Cat. No. C7026) be used on organoids in Matrigel medium?

CyQUANT Cell Proliferation Assay Kit (Cat. No. C7026) has been validated for use with the AlgiMatrix 3D Culture System, but we cannot guarantee that it will work with other 3D culture systems.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the composition of the cell lysis buffer in CyQUANT Cell Proliferation Assay, for cells in culture (Cat. No. C7026)?

The composition of the cell lysis buffer in CyQUANT Cell Proliferation Assay, for cells in culture is proprietary.

Which cell proliferation assays can I use with 3D culture systems?

alamarBlue Cell Viability Reagent, PrestoBlue Cell Viability Reagent, and CyQUANT Cell Proliferation Assay Kit (Cat. No. C7026) have been validated for use with the AlgiMatrix 3D Culture System and should also work with other 3D culture systems. For further details, please refer to this poster (https://aacrjournals.org/cancerres/article/70/8_Supplement/3234/563757/Abstract-3234-AlgiMatrixT-3-D-cell-culture-system) and protocol (http://www.thermofisher.com/us/en/home/references/protocols/cell-culture/3-d-cell-culture-protocol/algimatrix-viability-using-cyquant.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (65)

Citations et références
Abstract
Growth factor-induced proliferation in corneal epithelial cells is mediated by 12(S)-HETE.
Authors:Ottino P,Taheri F,Bazan HE
Journal:Experimental eye research
PubMed ID:12697425
PURPOSE: Previous studies in our laboratory have shown that 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE), a product of 12-lipoxygenase (12-LOX) activity, is the predominant metabolite formed in rabbit corneas after injury. The present study was undertaken to investigate the effects of epidermal growth factor (EGF), hepatocyte growth factor (HGF), and keratinocyte growth factor ... More
Caspase activation contributes to delayed death of heat-stressed striatal neurons.
Authors:White MG, Emery M, Nonner D, Barrett JN
Journal:J Neurochem
PubMed ID:14622126
'Hyperthermia can contribute to brain damage both during development and post-natally. We used rat embryonic striatal neurons in culture to study mechanisms underlying hyperthermia-induced neuronal death. Heat stress at 43 degrees C for 2 h produced no obvious signs of damage during the first 12 h after the stress, but ... More
Endothelial cell surface F1-F0 ATP synthase is active in ATP synthesis and is inhibited by angiostatin.
Authors:Moser TL, Kenan DJ, Ashley TA, Roy JA, Goodman MD, Misra UK, Cheek DJ, Pizzo SV
Journal:Proc Natl Acad Sci U S A
PubMed ID:11381144
'Angiostatin blocks tumor angiogenesis in vivo, almost certainly through its demonstrated ability to block endothelial cell migration and proliferation. Although the mechanism of angiostatin action remains unknown, identification of F(1)-F(O) ATP synthase as the major angiostatin-binding site on the endothelial cell surface suggests that ATP metabolism may play a role ... More
Overexpression of Na(+)/K (+)-ATPase parallels the increase in sodium transport and potassium recycling in an in vitro model of proximal tubule cellular ageing.
Authors:Silva E, Gomes P, Soares-da-Silva P,
Journal:J Membr Biol
PubMed ID:17334838
'Na(+)/K(+)-ATPase plays a key role in the transport of Na(+) throughout the nephron, but ageing appears to be accompanied by changes in the regulation and localization of the pump. In the present study, we examined the effect of in vitro cell ageing on the transport of Na(+) and K(+) ions ... More
Identification of the binding site for fibrinogen recognition peptide gamma 383-395 within the alpha(M)I-domain of integrin alpha(M)beta2.
Authors:Yakubenko VP, Solovjov DA, Zhang L, Yee VC, Plow EF, Ugarova TP
Journal:J Biol Chem
PubMed ID:11278633
'The leukocyte integrin alpha(M)beta(2) (Mac-1, CD11b/CD18) is a cell surface adhesion receptor for fibrinogen. The interaction between fibrinogen and alpha(M)beta(2) mediates a range of adhesive reactions during the immune-inflammatory response. The sequence gamma(383)TMKIIPFNRLTIG(395), P2-C, within the gamma-module of the D-domain of fibrinogen, is a recognition site for alpha(M)beta(2) and alpha(X)beta(2). ... More
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