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Citations et références (22)
Invitrogen™
DQ™ Green BSA - Special Packaging
DQ Green BSA est un substrat fluorogénique pour les protéases. Un important effet d’absorption de la fluorescence est observé lorsqueAfficher plus
| Référence | Quantité |
|---|---|
| D12050 | 5 x 1 mg |
Référence D12050
Prix (EUR)
710,65
Online Exclusive
808,00Économisez 97,35 (12%)
Each
Quantité:
5 x 1 mg
Prix (EUR)
710,65
Online Exclusive
808,00Économisez 97,35 (12%)
Each
DQ Green BSA est un substrat fluorogénique pour les protéases. Un important effet d’absorption de la fluorescence est observé lorsque les protéines sont fortement marquées avec des colorants BODIPY. Lors de l’hydrolyse du BSA vert DQ en peptides uniques marqués au colorant par des protéases, cette extinction est soulagée, produisant des produits fluorescents brillants.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Gamme de produitsDQ
Quantité5 x 1 mg
Conditions d’expéditionTempérature ambiante
SubstratSubstrat à base de protéase
Méthode de détectionFluorescent
FormePleine
Substrate PropertiesSubstrat à base de protéines
Unit SizeEach
Contenu et stockage
Conserver au congélateur (-5 à -30°C) à l’abri de la lumière.
Citations et références (22)
Citations et références
Abstract
Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafficking.
Journal:Nat Cell Biol
PubMed ID:18552835
'Autophagic and endocytic pathways are tightly regulated membrane rearrangement processes that are crucial for homeostasis, development and disease. Autophagic cargo is delivered from autophagosomes to lysosomes for degradation through a complex process that topologically resembles endosomal maturation. Here, we report that a Beclin1-binding autophagic tumour suppressor, UVRAG, interacts with the
A cytosolic calcium transient is not necessary for degranulation or oxidative burst in immune complex-stimulated neutrophils.
Journal:J Leukoc Biol
PubMed ID:9307071
'Receptor-mediated activation of neutrophils (PMN) initiates possibly interdependent events, including a rapid transient increase in [Ca2+]i, implicated as a second messenger. To investigate whether this transient is required for eventual degranulation, PMN were incubated with an intracellular Ca2+ chelator (BAPTA), then exposed to chemotactic peptide [N-formyl-methionyl-leucyl-phenylalanine (fMLP)l with or without
Maturation and trafficking of monocyte-derived dendritic cells in monkeys: implications for dendritic cell-based vaccines.
Journal:J Immunol
PubMed ID:10679086
'Human dendritic cells (DC) have polarized responses to chemokines as a function of maturation state, but the effect of maturation on DC trafficking in vivo is not known. We have addressed this question in a highly relevant rhesus macaque model. We demonstrate that immature and CD40 ligand-matured monocyte-derived DC have
A novel methodology for the investigation of intracellular proteolytic processing in intact cells.
Journal:Eur J Cell Biol
PubMed ID:9548376
'Taking advantage of the unique spectral properties of the fluorescent probe FL-Bodipy, we have developed a new methodology to study processing of exogenous proteins in intact cells. FL-Bodipy was conjugated to bovine serum albumin (BSA) at a molar ratio of 29 probe molecules to 1 albumin equivalent. The resulting conjugate
Assays to assess autophagy induction and fusion of autophagic vacuoles with a degradative compartment, using monodansylcadaverine (MDC) and DQ-BSA.
Journal:Methods Enzymol
PubMed ID:19200877
In this chapter we describe the use of monodasylcadaverine (MDC) and DQ-BSA, two practical and convenient tools to study the autophagic pathway. MDC is a lysosomotropic compound useful for the identification of autophagic vesicles by fluorescence microscopy and, in addition, to assess autophagy induction via the accumulation of MDC-labeled vacuoles.