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Citations et références (34)
Invitrogen™
C12FDG (5-dodécanoylaminofluorescéine di-β-D-galactopyranoside)
Le substrat d’β-galactosidase, C12FDG, a été modifié par covalence pour inclure une fraction lipophile de carbone-12. Une fois à l’intérieurAfficher plus
| Référence | Quantité |
|---|---|
| D2893 | 5 mg |
Référence D2893
Prix (EUR)
920,00
Each
Quantité:
5 mg
Prix (EUR)
920,00
Each
Le substrat d’β-galactosidase, C12FDG, a été modifié par covalence pour inclure une fraction lipophile de carbone-12. Une fois à l’intérieur de la cellule, le substrat est clivé par la β-galactosidase, produisant ainsi un produit fluorescent bien retenu par les cellules, et ce, grâce à la probable pénétration de la queue lipophile dans la membrane cellulaire. Le C12FDG est le composant principal du kit Imagene™ Green.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Excitation / émission490⁄514
Quantité5 mg
Conditions d’expéditionTempérature ambiante
Substratsubstrat Beta-Gal
Méthode de détectionFluorescent
Substrate Propertiessubstrat chimique
Unit SizeEach
Contenu et stockage
Conserver au congélateur (-5 et -30°C).
Citations et références (34)
Citations et références
Abstract
Rapid flow cytometric method for measuring senescence associated beta-galactosidase activity in human fibroblasts.
Journal:Cytometry A
PubMed ID:19777541
'Senescence associated-beta-galactosidase (SA-beta-gal) activity is a widely used marker for cellular senenescence. SA-beta-gal activity is routinely detected cytochemically, manually discriminating negative from positive cells. This method is time-consuming, subjective and therefore prone to operator-error. We aimed to optimize a flow cytometric method described by other workers using endothelial cells to
Optical imaging fiber-based single live cell arrays: a high-density cell assay platform.
Journal:Anal Chem
PubMed ID:12141663
'A high-density, ordered array containing thousands of microwells is fabricated on an optical imaging fiber. Each individually addressable microwell is used to accommodate a single living cell. A charged coupled device (CCD) detector is employed to monitor and spatially resolve the fluorescence signals obtained from each individual cell, allowing simultaneous
An optimized electroporation protocol applicable to a wide range of cell lines.
Journal:Biotechniques
PubMed ID:7873174
'A number of transfection methods for mammalian cells are available; however, many cell lines may appear resistant to efficient transfection, or at best, necessitate lengthy optimization procedures in recommended protocols. We describe here an electroporation protocol that yields highly efficient gene transfer (20%-100% of surviving cells) in all 19 cell
Mapping mechanisms and charting the time course of premature cell senescence and apoptosis: lysosomal dysfunction and ganglioside accumulation in endothelial cells.
Journal:Am J Physiol Renal Physiol
PubMed ID:17928415
'Endothelial cells subjected to glycated collagen I develop premature senescence within 3-5 days, as revealed by increased senescence-associated beta-galactosidase activity, decreased proliferation, and an increase in cell size. Here, we analyzed the time course and possible mechanisms of this process. Lysosomal integrity studies revealed a rapid collapse of pH gradient
Use of fluorescence-activated cell sorting for rapid isolation of insect cells harboring recombinant baculovirus.
Journal:Methods Cell Biol
PubMed ID:7877509