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Citations et références (31)
Invitrogen™
β-BODIPY™ FL C12-HPC (2-(4,4-difluoro-5,7-diméthyl-4-bora-3a,4a-diaza-s-indacène-3-dodécanoyl)-1-hexadécanoyl-sn-glycéro-3-phosphocholine)
Le fluorophore BODIPY® (4,4-difluoro-3a,4a-diaza-s-indacène) intensément fluorescent est intrinsèquement lipophile, contrairement à la plupart des autres colorants à longue longueur d’onde.Afficher plus
| Référence | Quantité |
|---|---|
| D3792 | 100 μg |
Référence D3792
Prix (EUR)
237,00
Each
Quantité:
100 μg
Prix (EUR)
237,00
Each
Le fluorophore BODIPY® (4,4-difluoro-3a,4a-diaza-s-indacène) intensément fluorescent est intrinsèquement lipophile, contrairement à la plupart des autres colorants à longue longueur d’onde. Par conséquent, les sondes incorporant ce fluorophore sont plus susceptibles d’imiter les propriétés des lipides naturels. Les analogues d’acides gras BODIPY® tels que l’acide dodécanoïque BODIPY® 500 / 510 (D-3823) et l’acide dodécanoïque BODIPY FL (D-3822) subissent un transport et un métabolisme natifs dans les cellules. Ils sont donc des traceurs efficaces de trafic des lipides, ainsi que des sondes à membrane polyvalentes utiles.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Nom chimique ou matériauPhospholipides
Température de stockageConserver au congélateur (-5 à -30°C) à l’abri de la lumière.
Forme physiqueSolid
Gamme de produitsBODIPY
Quantité100 μg
Unit SizeEach
Citations et références (31)
Citations et références
Abstract
Separation and characterization of late endosomal membrane domains.
Journal:J Biol Chem
PubMed ID:12065580
'Very little is known about the biophysical properties and the lipid or protein composition of membrane domains presumably present in endocytic and biosynthetic organelles. Here we analyzed the membrane composition of late endosomes by suborganellar fractionation in the absence of detergent. We found that the internal membranes of this multivesicular
Multidrug transporter activity in lymphocytes.
Journal:Br J Pharmacol
PubMed ID:15492020
'Multidrug transporters play a dual role in haematopoietic cells, mediating the efflux of xenobiotics and regulating cell migration. For several reasons including the lack of specific antibodies, reports of multidrug transporter distribution on lymphocytes conflict. Murine B cells have been reported to completely lack transporter activity. Through analysis of parental
gamma-cyclodextrins greatly enhance translocation of hydrophobic fluorescent phospholipids from vesicles to cells in culture. Importance of molecular hydrophobicity in phospholipid trafficking studies.
Journal:J Biol Chem
PubMed ID:10585403
'Short-chain, fluorescent derivatives are commonly used to investigate intracellular phospholipid trafficking. However, their use can yield misleading results because they, unlike the native species, can rapidly distribute between organelles due to their low hydrophobicity. On the other hand, hydrophobic derivatives are very difficult to introduce to cells and thus have
Fluorescent membrane probes incorporating dipyrrometheneboron difluoride fluorophores.
Journal:Anal Biochem
PubMed ID:1799206
'The spectroscopic properties of a new series of fatty acid analogs in which a dipyrrometheneboron difluoride fluorophore forms a segment of the acyl methylene chain are presented and their characteristics as fluorescent membrane probes are examined. When incorporated as a low mole fraction component in model phospholipid membranes, the probes
Intracellular surfactant removal from phagocytized minerals: development of a fluorescent method using a BODIPY-labeled phospholipid.
Journal:Inhal Toxicol
PubMed ID:10880156
'Lung surfactant serves as a protective coating when adsorbed on particle surfaces, so its removal or rate of removal in vivo may affect expression of mineral cytotoxicity. Removal of phospholipid surfactant components from the surface of mineral particles ingested by alveolar macrophages (AM) was measured using fluorescence microscopy. Dipalmitoylphosphatidylcholine with