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Citations et références (181)
Invitrogen™
DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)
DTNB ; réactif d’Ellman, 5,5’-dithiobis-(acide 2-nitrobenzoïque)
| Référence | Quantité |
|---|---|
| D8451 | 10 g |
Référence D8451
Prix (EUR)
257,00
Each
Quantité:
10 g
Prix (EUR)
257,00
Each
Le DTNB ou réactif d’Elman peut être utilisé pour quantifier les thiols dans les protéines, les cellules et le plasma par des mesures d’absorption. Il forme facilement un disulfure mélangé avec des thiols, libérant l’acide chromophore 5-merapto-2-nitrobenzoïque (absorption maximale de 410 nm). Seuls les thiols protéiques accessibles à ce réactif hydrosoluble sont modifiés.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Quantité10 g
Type de produitRéactif d’Elman
Unit SizeEach
Citations et références (181)
Citations et références
Abstract
Spatial proximity of Cys113, Cys172, and Cys422 in the metalloactivation domain of the ArsA ATPase.
Journal:The Journal of biological chemistry
PubMed ID:8798705
A general strategy for site-specific double labeling of globular proteins for kinetic FRET studies.
Journal:Bioconjugate chemistry
PubMed ID:12236801
Site-directed mutagenesis provides a straightforward means of creating specific targets for chemical modifications of proteins. This capability enhanced the applications of spectroscopic methods adapted for addressing specific structural questions such as the characterization of partially folded and transient intermediate structures of globular proteins. Some applications such as the steady state
Reactivity of mitochondrial sulfhydryl groups toward dithionitrobenzoic acid and bromobimanes under oligomycin-inhibited and uncoupling conditions.
Journal:Arch Biochem Biophys
PubMed ID:2845867
Thiol reactivity was determined in rat heart mitochondria using chromophores of differing polarities: monobromobimane (MB), dithionitrobenzoate (NbS2), and bromobimane-q (MQ). The purpose of this study is to correlate reaction rates of protein thiols in the mitochondrial membrane with the oligomycin-inhibited and uncoupled states: In all cases investigated the reactivity of
Sulfhydryl groups of rabbit liver arylsulfatase A.
Journal:Biochim Biophys Acta
PubMed ID:2864083
Rabbit liver arylsulfatase A (aryl-sulfate sulfhydrolase, EC 3.1.6.1) monomers of 130 kDa contain two free sulfhydryl groups as determined by spectrophotometric titration using 5,5'-dithiobis(2-nitrobenzoate) and by labeling with the fluorescent probe 5-(iodoacetamidoethyl)aminonaphthalene-1-sulfonic acid. Fluorescence quenching data indicate that the reactive sulfhydryl is present in proximity to one or more tryptophan
Actin can act as a cofactor for a viral proteinase in the cleavage of the cytoskeleton.
Journal:J Biol Chem
PubMed ID:12191991
'Cytoskeletal proteins are exploited by many viruses during infection. We report a novel finding that actin can act as a cofactor for the adenovirus proteinase (AVP) in the degradation of cytoskeletal proteins. Transfection studies in HeLa cells revealed AVP localized with cytokeratin 18, and this was followed by destruction of