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Thermo Scientific™
E. coli DNA Ligase
Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.
| Référence | Quantité |
|---|---|
| EL00361 | 400 U |
Référence EL00361
Prix (EUR)
119,00
Each
Quantité:
400 U
Prix (EUR)
119,00
Each
Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. The enzyme is not active on blunt-ended DNA.
Features
- Activity in wide temperature range (4°C to 37°C)
- High specificity to cohesive DNA ends
Applications
- Nick ligation in double-stranded DNA
- cDNA synthesis
- Cloning
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
À utiliser avec (application)Nick ligation in double-stranded DNA, cDNA synthesis, Cloning, DNA ligation
Inactivation Temperature65°C
Type de produitNAD-dependent DNA Ligase
Quantité400 U
Concentration8 U/μL
EnzymesADN ligase
FormeLiquide
Catégorie de rechercheTraditional Cloning
Sensible à l’inactivation thermiqueOui
Unit SizeEach
Contenu et stockage
8 U/μL E.Coli DNA Ligase, 50 μL
Store at -20°C.
Store at -20°C.