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Thermo Scientific™
Taq DNA Polymerase, native (5 U/μL)
Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.
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| Référence | Format |
|---|---|
| EP0282 | Without BSA |
| EP0072 | With BSA |
Référence EP0282
Prix (EUR)
254,00
Each
Format:
Without BSA
Prix (EUR)
254,00
Each
Thermo Scientific Taq DNA Polymerase, native, is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.
Available with or without the stabilizing agent BSA, Taq DNA Polymerase, native, with BSA, is often considered the best choice when amplifying DNA samples of lower purity, e.g., genomic DNA from mouse tail.
Both native Taq DNA polymerases are supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at a wide range of magnesium concentrations and decreases unspecific priming
Features
- Thermostable with half-life more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides)
Applications
- Routine PCR amplification of DNA fragments up to 5 kb
- DNA labeling
- High throughput PCR
Notes
- The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
- The 10X Taq Buffer without Detergent is recommended for microarray experiments.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Fidélité (par rapport à Taq)1 X
FormatWithout BSA
Hot StartNon
En surplomb3’-A
PolyméraseADN polymérase Taq
Type de produitADN polymérase
Quantité500 unités
Format de réactionComposants séparés
Matériau de départADN
Volume2 mL
Concentration10X
À utiliser avec (application)PCR standard
GC-Rich PCR PerformanceFaible
Vitesse de réactionÉtalon
Unit SizeEach
Contenu et stockage
Contenu :
• 100 μl d’ADN polymérase Taq, native (5 U/μl)
• 2 x 1,25 ml de tampon Taq 10X avec KCl
• 2 x 1,25 ml de tampon Taq 10X avec (NH4)2SO4
• 2 x 1,25 ml de MgCl2 à 25 mM
Conserver à -20°C.
• 100 μl d’ADN polymérase Taq, native (5 U/μl)
• 2 x 1,25 ml de tampon Taq 10X avec KCl
• 2 x 1,25 ml de tampon Taq 10X avec (NH4)2SO4
• 2 x 1,25 ml de MgCl2 à 25 mM
Conserver à -20°C.