FluoCells™ Prepared Slide #2 (BPAE cells with Mouse Anti-α-tubulin, AlexaFluor™ 488, FL Goat Anti-Mouse IgG, Texas Red™-X Phalloidin, and DAPI)

Les lames de microscope préparées FluoCells contiennent des préparations de cellules multi-marquées pour observation par microscopie par fluorescence. La lameAfficher plus

Référence	Quantité
F14781	1/boîte

Référence F14781

Prix (EUR)

414,00

Quantité:

1/boîte

Prix (EUR)

414,00

Les lames de microscope préparées FluoCells contiennent des préparations de cellules multi-marquées pour observation par microscopie par fluorescence. La lame préparée FluoCells n°2 contient des cellules endothéliales d’artère pulmonaire bovine (BPAEC). La phalloïdine Texas Red-X marque l’anticorps monoclonal de souris actine F, l’anticorps monoclonal de souris anti-bovin α-tubuline 236-10501 , en association avec l’anticorps d’IgG anti-souris de chèvre BODIPY FL, marque les microtubules et la DAPI marque le noyau.

Consultez notre collection complète de réactifs d’étalonnage de microscope ›

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

À utiliser avec (application)Immunofluorescence

Quantité1/boîte

Conditions d’expéditionTempérature ambiante

Gamme de produitsBODIPY, FluoCells, Texas Red

TypeLame de microscope préparée

Unit SizeEach

Contenu et stockage

Conserver à température ambiante et à l’abri de la lumière.

Foire aux questions (FAQ)

Do you have any slides of pre-labeled cells or tissues that I can use to demonstrate with?

Some of the most popular are the FluoCells demonstration slides, which have cells or tissue sections that are labeled with three different dyes. FluoCells 1 and 2 have cultured cells, while FluoCells 3 is a tissue section (mouse kidney). Slides with fluorescent beads are also available.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (1)

Citations et références

Abstract

Practical confocal microscopy and the evaluation of system performance.

Authors:Zucker RM, Price OT

Journal:Methods

PubMed ID:10491274

'The laser scanning confocal microscope has enormous potential in many fields of biology. Currently there is a subjective nature in the assessment of a confocal microscope''s performance by primarily evaluating the system with a specific test slide provided by the user''s laboratory. To achieve better performance from the equipment, it ... More