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Citations et références (37)
Invitrogen™
Fluorescein Diphosphate, Tetraammonium Salt (FDP)
Le FDP est un substrat incolore et non fluorescent pour les phosphatases alcalines. L’hydrolyse séquentielle médiée par les phosphatases alcalinesAfficher plus
| Référence | Quantité |
|---|---|
| F2999 | 5 mg |
Référence F2999
Prix (EUR)
778,00
Each
Quantité:
5 mg
Prix (EUR)
778,00
Each
Le FDP est un substrat incolore et non fluorescent pour les phosphatases alcalines. L’hydrolyse séquentielle médiée par les phosphatases alcalines de ses deux substituants de phosphate produit un monophosphate de fluorescéine faiblement fluorescent suivi d’une fluorescéine fortement fluorescente (excitation/émission ∼ 490/514 nm).
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Excitation / émission490⁄514
À utiliser avec (équipement)Fluorimètre, lecteur de microplaques
Marqueur ou colorantFITC (fluorescéine)
Quantité5 mg
Conditions d’expéditionTempérature ambiante
SubstratSubstrat de phosphatase
Méthode de détectionFluorescent
FormePoudre
Substrate Propertiessubstrat chimique
Target EnzymePhosphatase alcaline, phosphatases tyrosine
Unit SizeEach
Contenu et stockage
Conserver au congélateur (-5 à -30 °C).
Citations et références (37)
Citations et références
Abstract
Glibenclamide prevents increased extracellular matrix formation induced by high glucose concentration in mesangial cells.
Journal:Am J Physiol Renal Physiol
PubMed ID:16896180
'Other than stimulation of cell contractility, little is known about the potential metabolic effects induced by sulfonylureas, independently of insulin action. Previous studies from our laboratory demonstrated complete abrogation of glomerulosclerosis in an experimental model of type 1 diabetes chronically (9 mo) treated with low-dose sulfonylureas (Biederman JI, Vera E,
Negative regulation of EGFR signalling through integrin-alpha1beta1-mediated activation of protein tyrosine phosphatase TCPTP.
Journal:Nat Cell Biol
PubMed ID:15592458
'Integrin-mediated cell adhesion regulates a multitude of cellular responses, including proliferation, survival and cross-talk between different cellular signalling pathways. So far, integrins have been mainly shown to convey permissive signals enabling anchorage-dependent receptor tyrosine kinase signalling. Here we show that a collagen-binding integrin alpha(1)beta(1) functions as a negative regulator of
Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation.
Journal:Anal Chem
PubMed ID:12199607
'A tool for detection and characterization of intracellular enzyme-substrate and receptor-ligand interactions inside the cytoplasm of single targeted cells or small confined groups of cells is presented. Fluorogenic enzyme substrates and receptor ligands were rapidly delivered by electroosmosis and internalized by electroporation in cells using an electrolyte-filled capillary (EFC) biased
Randomly ordered addressable high-density optical sensor arrays.
Journal:Anal Chem
PubMed ID:9553489
'Array-based sensors provide an architecture for multianalyte sensing. In this paper, we report a new approach for array fabrication. Sensors are made by immobilizing different reactive chemistries on the surfaces of microspheres. Sensor arrays are prepared by randomly distributing a mixture of microsphere sensors on an optical substrate containing thousands
In vivo imaging of tumors with protease-activated near-infrared fluorescent probes.
Journal:Nat Biotechnol
PubMed ID:10207887
'We have developed a method to image tumor-associated lysosomal protease activity in a xenograft mouse model in vivo using autoquenched near-infrared fluorescence (NIRF) probes. NIRF probes were bound to a long circulating graft copolymer consisting of poly-L-lysine and methoxypolyethylene glycol succinate. Following intravenous injection, the NIRF probe carrier accumulated in