Phusion™ HF and GC Buffer Packs
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Thermo Scientific™

Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
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RéférenceNom chimique ou matériauCouleur
F518LHF Buffer PackColorless
F519LGC Buffer PackColorless
F520LDetergent-free FF Buffer PackColorless
F521LDetergent-free GC Buffer PackColorless
F538LHF Buffer PackGreen
F539LGC Buffer PackGreen
Référence F518L
Prix (EUR)
35,37
Each
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Nom chimique ou matériau:
HF Buffer Pack
Couleur:
Colorless
Grand volume ou format personnalisé
Prix (EUR)
35,37
Each
Ajouter au panier
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Nom chimique ou matériauHF Buffer Pack
CouleurColorless
DescriptionBoîte de tampons Phusion™ HF
ComprendLe pack de tampon Phusion HF (F-518L) comprend :
• Tampon Phusion HF 5X (tampon 1X fournissant 1,5 mM de MgCl2)
• 50 mM de MgCl2
• DMSO

Conserver à -20°C.
À utiliser avec (application)PCR standard
Quantité6 mL
Unit SizeEach