FluoSpheres™ Carboxylate-Modified Microspheres

Citations et références (22)

Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F8803
Price: 728.00 EUR

Réalisez la fluorescence la plus brillante grâce aux microsphères FluoSphere modifiées au carboxylate, disponibles dans différentes couleurs et tailles de particules.

Modifier l'affichage

Référence	Diamètre (métrique)	Couleur	Excitation / émission	Quantité
F8803	0,1 μm	Jaune-vert	505/515 nm	10 mL
F8816	1,0 μm	Cramoisi	625/645 nm	2 mL
F8823	1,0 μm	Jaune-vert	505/515 nm	10 mL
F8801	0,1 μm	Rouge	580/605 nm	10 mL
F8811	0,2 μm	Jaune-vert	505/515 nm	10 mL
F8807	0,2 μm	Rouge foncé	660/680 nm	2 mL
F10720	0,04 μm	Vert jaune, Orange, Rouge, Rouge foncé	505 / 515, 540 / 560, 580 / 605, 660 / 680 nm	1 ml (chacun)
F20881	0,2 μm	Orange	365/610 nm	2 mL
F8783 également connu sous le numéro F-8783	0,02 μm	Rouge foncé	660/680 nm	2 mL
F8786	0,02 μm	Couleur rouge	580/605 nm	10 mL
F8795 également connu sous le numéro F-8795	0,04 μm	Vert jaune	505/515 nm	1 mL
F8813	0,5 μm	Jaune-vert	505/515 nm	10 mL
F8820	1,0 μm	Orange	540/560 nm	10 mL
F8825	2,0 μm	Rouge du Nil	535/575 nm	2 mL
F8827	2,0 μm	Jaune-vert	505/515 nm	2 mL
F8781 également connu sous le numéro F-8781	0,02 μm	Bleu	365/415 nm	10 mL
F8782 également connu sous le numéro F-8782	0,02 μm	Cramoisi	625/645 nm	2 mL
F8784	0,02 μm	Rouge du Nil	535/575 nm	10 mL
F8787	0,02 μm	Jaune-vert	505/515 nm	10 mL
F8789 également connu sous le numéro F-8789	0,04 μm	Rouge foncé	660/680 nm	1 mL
F8792 également connu sous le numéro F-8792	0,04 μm	Orange	540/560 nm	1 mL
F8793	0,04 μm	Couleur rouge	580/605 nm	1 mL
F8794	0,04 μm	Rouge-orange	565/580 nm	1 mL
F8797	0,1 μm	Couleur bleue	350/440 nm	10 mL
F8799	0,1 μm	Infrarouge	715/755 nm	1 mL
F8800 également connu sous le numéro F-8800	0,1 μm	Orange	540/560 nm	10 mL
F8805	0,2 μm	Couleur bleue	365/415 nm	10 mL
F8806	0,2 μm	Cramoisi	625/645 nm	2 mL
F8809	0,2 μm	Orange	540/560 nm	10 mL
F8810	0,2 μm	Rouge	580/605 nm	10 mL
F8812	0,5 μm	Rouge	580/605 nm	10 mL
F8814	1,0 μm	Couleur bleue	365/415 nm	10 mL
F8815	1,0 μm	Couleur bleue	350/440 nm	10 mL
F8819	1,0 μm	Rouge du Nil	535/575 nm	10 mL
F8821	1,0 μm	Rouge	580/605 nm	10 mL
F8824 également connu sous le numéro F-8824	2,0 μm	Couleur bleue	365/415 nm	2 mL
F8826	2,0 μm	Couleur rouge	580/605 nm	2 mL

37 options

Référence F8803

Prix (EUR)

728,00

Each

Diamètre (métrique):

0,1 μm

Couleur:

Jaune-vert

Excitation / émission:

505/515 nm

Quantité:

10 mL

Prix (EUR)

728,00

Each

Exécutez facilement cytométrie en flux, microscopie, HTS, HCS, immunodosage et autres applications de laboratoire grâce à notre vaste sélection de microsphères FluoSphere modifiées au carboxylate. Les microbilles FluoSphere peuvent être utilisées pour l’adsorption passive ou active, le couplage covalent de protéines, d’acides nucléiques et de biomolécules pour les applications de capture de particules. Les microsphères FluoSphere sont chargées avec des colorants fluorescents exclusifs, ce qui en fait les microsphères les plus brillantes disponibles.

Visualisez la fluorescence la plus brillante pour les applications de laboratoire, notamment la microscopie par fluorescence, la cytométrie en flux, le HTS, le HCS et le traçage cellulaire, grâce à nos microsphères FluoSphere modifiées au carboxylate, fabriquées à partir de microsphères en polystyrène et chargées avec différents colorants exclusifs. Grâce à des méthodes de coloration spécialisées, toutes les molécules de colorants fluorescents sont contenues à l’intérieur de chaque microsphère en polystyrène, au lieu de se trouver à la surface des microbilles. Cet environnement protecteur dans les microbilles protège le colorant contre les effets environnementaux préjudiciables, tels que le photoblanchiment. Nos microsphères modifiées au carboxylate sont revêtues d’un polymère hydrophile contenant plusieurs acides carboxyliques pour l’adhérence covalente des ligands. Une variété de tailles de particules sont disponibles pour différentes utilisations et expériences de recherche.

For Research Use Only. Not for use in diagnostic procedures.

Spécifications

Excitation / émission505/515 nm

Gamme de produitsFLUOSPHERES

Quantité10 mL

Modification de surfaceCarboxylate

CouleurJaune-vert

Diamètre (métrique)0,1 μm

À utiliser avec (application)Microscopie par fluorescence

MatériauPolystyrène

Type de produitMicrosphère au carboxylate modifié

Unit SizeEach

Contenu et stockage

Conserver au réfrigérateur (entre 2 et 8°C) à l’abri de la lumière.

Foire aux questions (FAQ)

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When does the warranty guarantee expire for FluoSpheres Carboxylated-Modified Microspheres?

FluoSpheres Carboxylate-Modified Microspheres (Cat. Nos. F8783, F8786, F8801, F8807, F8811, F8803, F8816, F8823, F20881, F10720) have a 1-year warranty guarantee, unless otherwise indicated on the Certificate of Analysis (COA).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

Citations et références (22)

Citations et références

Abstract

Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination.

Authors:Gustafsson MG, Shao L, Carlton PM, Wang CJ, Golubovskaya IN, Cande WZ, Agard DA, Sedat JW,

Journal:Biophys J

PubMed ID:18326650

'Structured illumination microscopy is a method that can increase the spatial resolution of wide-field fluorescence microscopy beyond its classical limit by using spatially structured illumination light. Here we describe how this method can be applied in three dimensions to double the axial as well as the lateral resolution, with true ... More

The power of single and multibeam two-photon microscopy for high-resolution and high-speed deep tissue and intravital imaging.

Authors:Niesner R, Andresen V, Neumann J, Spiecker H, Gunzer M,

Journal:Biophys J

PubMed ID:17557785

'Two-photon microscopy is indispensable for deep tissue and intravital imaging. However, current technology based on single-beam point scanning has reached sensitivity and speed limits because higher performance requires higher laser power leading to sample degradation. We utilize a multifocal scanhead splitting a laser beam into a line of 64 foci, ... More

Role of glycocalyx in leukocyte-endothelial cell adhesion.

Authors:Mulivor AW, Lipowsky HH

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:12234777

'The binding of fluorescently labeled microspheres (FLMs, 0.1-microm diameter) coated with antibody (1a29) to ICAM-1 was studied in postcapillary venules during topical application of the chemoattractant N-formylmethionyl-leucyl-phenylalanine (fMLP). FLM adhesion to endothelial cells (ECs) increased dramatically from 50 to 150 spheres per 100-microm length of venule after superfusion of the ... More

a2 Adrenergic receptor-mediated inhibition of thermogenesis.

Authors:Madden CJ, Tupone D, Cano G, Morrison SF,

Journal:J Neurosci

PubMed ID:23365239

'a2 adrenergic receptor (a2-AR) agonists have been used as antihypertensive agents, in the management of drug withdrawal, and as sedative analgesics. Since a2-AR agonists also influence the regulation of body temperature, we explored their potential as antipyretic agents. This study delineates the central neural substrate for the inhibition of rat ... More

Glucoprivation in the ventrolateral medulla decreases brown adipose tissue sympathetic nerve activity by decreasing the activity of neurons in raphe pallidus.

Authors:Madden CJ,

Journal:Am J Physiol Regul Integr Comp Physiol

PubMed ID:22071154

'In urethane/a-chloralose anesthetized rats, cold exposure increased brown adipose tissue sympathetic nerve activity (BAT SNA: +699 ± 104% control). Intravenous administration of 2-deoxy-D-glucose (2-DG; 200 mg·ml(-1)·kg(-1)) reversed the cold-evoked activation of BAT SNA (nadir: 139 ± 36% of control) and decreased BAT temperature (-1.1 ± 0.2°C), expired CO(2) (-0.4 ± ... More

View all FluoSpheres™ Carboxylate-Modified Microspheres citations

FluoSpheres™ Carboxylate-Modified Microspheres

Foire aux questions (FAQ)

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

I sonicated my 2.0 &micro;m carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

When does the warranty guarantee expire for FluoSpheres Carboxylated-Modified Microspheres?

What is the warranty for FluoSpheres microspheres?

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Citations et références (22)

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?