FluoSpheres™ Carboxylate-Modified Microspheres

Citations et références (4)

Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Name: FluoSpheres™ Carboxylate-Modified Microspheres
SKU: F8819
Price: 638.00 EUR

Réalisez la fluorescence la plus brillante grâce aux microsphères FluoSphere modifiées au carboxylate, disponibles dans différentes couleurs et tailles de particules.

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Référence	Diamètre (métrique)	Couleur	Excitation / émission	Quantité
F8819	1,0 μm	Rouge du Nil	535/575 nm	10 mL
F8803	0,1 μm	Jaune-vert	505/515 nm	10 mL
F8816	1,0 μm	Cramoisi	625/645 nm	2 mL
F8823	1,0 μm	Jaune-vert	505/515 nm	10 mL
F8801	0,1 μm	Rouge	580/605 nm	10 mL
F8811	0,2 μm	Jaune-vert	505/515 nm	10 mL
F8807	0,2 μm	Rouge foncé	660/680 nm	2 mL
F10720	0,04 μm	Vert jaune, Orange, Rouge, Rouge foncé	505 / 515, 540 / 560, 580 / 605, 660 / 680 nm	1 ml (chacun)
F20881	0,2 μm	Orange	365/610 nm	2 mL
F8783 également connu sous le numéro F-8783	0,02 μm	Rouge foncé	660/680 nm	2 mL
F8786	0,02 μm	Couleur rouge	580/605 nm	10 mL
F8795 également connu sous le numéro F-8795	0,04 μm	Vert jaune	505/515 nm	1 mL
F8813	0,5 μm	Jaune-vert	505/515 nm	10 mL
F8820	1,0 μm	Orange	540/560 nm	10 mL
F8825	2,0 μm	Rouge du Nil	535/575 nm	2 mL
F8827	2,0 μm	Jaune-vert	505/515 nm	2 mL
F8781 également connu sous le numéro F-8781	0,02 μm	Bleu	365/415 nm	10 mL
F8782 également connu sous le numéro F-8782	0,02 μm	Cramoisi	625/645 nm	2 mL
F8784	0,02 μm	Rouge du Nil	535/575 nm	10 mL
F8787	0,02 μm	Jaune-vert	505/515 nm	10 mL
F8789 également connu sous le numéro F-8789	0,04 μm	Rouge foncé	660/680 nm	1 mL
F8792 également connu sous le numéro F-8792	0,04 μm	Orange	540/560 nm	1 mL
F8793	0,04 μm	Couleur rouge	580/605 nm	1 mL
F8794	0,04 μm	Rouge-orange	565/580 nm	1 mL
F8797	0,1 μm	Couleur bleue	350/440 nm	10 mL
F8799	0,1 μm	Infrarouge	715/755 nm	1 mL
F8800 également connu sous le numéro F-8800	0,1 μm	Orange	540/560 nm	10 mL
F8805	0,2 μm	Couleur bleue	365/415 nm	10 mL
F8806	0,2 μm	Cramoisi	625/645 nm	2 mL
F8809	0,2 μm	Orange	540/560 nm	10 mL
F8810	0,2 μm	Rouge	580/605 nm	10 mL
F8812	0,5 μm	Rouge	580/605 nm	10 mL
F8814	1,0 μm	Couleur bleue	365/415 nm	10 mL
F8815	1,0 μm	Couleur bleue	350/440 nm	10 mL
F8821	1,0 μm	Rouge	580/605 nm	10 mL
F8824 également connu sous le numéro F-8824	2,0 μm	Couleur bleue	365/415 nm	2 mL
F8826	2,0 μm	Couleur rouge	580/605 nm	2 mL

37 options

Référence F8819

Prix (EUR)

638,00

Each

Diamètre (métrique):

1,0 μm

Couleur:

Rouge du Nil

Excitation / émission:

535/575 nm

Quantité:

10 mL

Prix (EUR)

638,00

Each

Exécutez facilement cytométrie en flux, microscopie, HTS, HCS, immunodosage et autres applications de laboratoire grâce à notre vaste sélection de microsphères FluoSphere modifiées au carboxylate. Les microbilles FluoSphere peuvent être utilisées pour l’adsorption passive ou active, le couplage covalent de protéines, d’acides nucléiques et de biomolécules pour les applications de capture de particules. Les microsphères FluoSphere sont chargées avec des colorants fluorescents exclusifs, ce qui en fait les microsphères les plus brillantes disponibles.

Visualisez la fluorescence la plus brillante pour les applications de laboratoire, notamment la microscopie par fluorescence, la cytométrie en flux, le HTS, le HCS et le traçage cellulaire, grâce à nos microsphères FluoSphere modifiées au carboxylate, fabriquées à partir de microsphères en polystyrène et chargées avec différents colorants exclusifs. Grâce à des méthodes de coloration spécialisées, toutes les molécules de colorants fluorescents sont contenues à l’intérieur de chaque microsphère en polystyrène, au lieu de se trouver à la surface des microbilles. Cet environnement protecteur dans les microbilles protège le colorant contre les effets environnementaux préjudiciables, tels que le photoblanchiment. Nos microsphères modifiées au carboxylate sont revêtues d’un polymère hydrophile contenant plusieurs acides carboxyliques pour l’adhérence covalente des ligands. Une variété de tailles de particules sont disponibles pour différentes utilisations et expériences de recherche.

For Research Use Only. Not for use in diagnostic procedures.

Spécifications

Excitation / émission535/575 nm

Gamme de produitsFLUOSPHERES

Quantité10 mL

Modification de surfaceCarboxylate

CouleurRouge du Nil

Diamètre (métrique)1,0 μm

À utiliser avec (application)Microscopie par fluorescence

MatériauPolystyrène

Type de produitMicrosphère au carboxylate modifié

Unit SizeEach

Contenu et stockage

Conserver au réfrigérateur (entre 2 et 8°C) à l’abri de la lumière.

Foire aux questions (FAQ)

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

The CML beads have a high density of carboxyl groups at the surface. The surface layer is quite hydrophilic and at the appropriate pH (pH>5), are charged; due to electrostatic repulsion, this type of surface is 3-dimensional and may be considered analogous to the fuzz on a tennis ball.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Carboxylate-Modified Microspheres FAQs

Citations et références (4)

Citations et références

Abstract

Absolute brightness of fluorescent microspheres.

Authors:Finger I, Phillips S, Mobley E, Tucker R, Hess H,

Journal:Lab Chip

PubMed ID:19156300

The absolute brightness of fluorescent particles, such as dye-containing nano- and microspheres or quantum dots, is a critical design parameter for many applications relying on fluorescence detection. The absolute brightness, defined as the ratio of radiant intensity of emission to illumination intensity of excitation, of nile-red fluorescent microspheres with a ... More

HAMI 3379, a CysLT2 receptor antagonist, attenuates ischemia-like neuronal injury by inhibiting microglial activation.

Authors:Zhang XY, Wang XR, Xu DM, Yu SY, Shi QJ, Zhang LH, Chen L, Fang SH, Lu YB, Zhang WP, Wei EQ,

Journal:

PubMed ID:23750020

The cysteinyl leukotrienes (CysLTs) are inflammatory mediators closely associated with neuronal injury after brain ischemia through the activation of their receptors, CysLT1R and CysLT2R. Here we investigated the involvement of both receptors in oxygen-glucose deprivation/recovery (OGD/R)-induced ischemic neuronal injury and the effect of the novel CysLT2R antagonist HAMI 3379 [3-({[(1S,3S)-3- ... More

Fidelity of adaptive phototaxis.

Authors:Drescher K, Goldstein RE, Tuval I,

Journal:Proc Natl Acad Sci U S A

PubMed ID:20534560

Along the evolutionary path from single cells to multicellular organisms with a central nervous system are species of intermediate complexity that move in ways suggesting high-level coordination, yet have none. Instead, organisms of this type possess many autonomous cells endowed with programs that have evolved to achieve concerted responses to ... More

Angiogenesis monitored by perfusion with a space-filling microbead suspension.

Authors:Springer ML, Ip TK, Blau HM

Journal:Mol Ther

PubMed ID:10933915

Numerous laboratories are focusing efforts on delivering gene products to induce or prevent the development of new blood vessels in adults, with the hope of rescuing ischemic tissues, circumventing cardiac bypass surgery, or inhibiting tumor growth. Current approaches to the assessment of vascular continuity involve the introduction of either dyes ... More

FluoSpheres™ Carboxylate-Modified Microspheres

Foire aux questions (FAQ)

I read that carboxylate-modified latex (CML) beads have a "fluffy coat" of carboxyl groups on their surface. What is meant by this?

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

I sonicated my 2.0 &micro;m carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

What is the warranty for FluoSpheres microspheres?

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Citations et références (4)

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?