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Citations et références (28)
Invitrogen™
Guanosine 5'-O-(3-Thiotriphosphate), BODIPY™ FL Thioester, Sodium Salt (BODIPY™ FL GTP-γ-S, Thioester)
BODIPY FL GTP-γ-S se lie aux protéines liant le GTP. Pour créer ce nucléotide fluorescent, le fluorophore BODIPY FL aAfficher plus
| Référence | Quantité |
|---|---|
| G22183 également connu sous le numéro G-22183 | 50 μl |
Référence G22183
également connu sous le numéro G-22183
Prix (EUR)
1 138,00
Each
Quantité:
50 μl
Prix (EUR)
1 138,00
Each
BODIPY FL GTP-γ-S se lie aux protéines liant le GTP. Pour créer ce nucléotide fluorescent, le fluorophore BODIPY FL a été lié par le γ-thiol de GTP-γ-S. La fluorescence de BODIPY FL GTP-γ-S est éteinte d’environ 90 % par rapport à celle du colorant libre, mais peut être récupérée sur la liaison aux protéines G. En plus de son utilisation potentielle pour les études de liaison, le thioéther BODIPY FL GTP-γ-S est un nouveau substrat important pour le Fhit, un membre de la superfamille de triades histidine de protéines liant les nucléotides.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Méthode d’étiquetageMarquage chimique, marquage direct
Marqueur ou colorantBODIPY™ FL
Type de produitGuanosine 5’-O
Quantité50 μl
Conditions d’expéditionGlace humide
Concentration5 mM
Gamme de produitsBODIPY
Unit SizeEach
Contenu et stockage
Stocker au congélateur (entre -5°C et -30°C) à l’abri de la lumière.
Citations et références (28)
Citations et références
Abstract
Detection of G proteins by affinity probe capillary electrophoresis using a fluorescently labeled GTP analogue.
Journal:Anal Chem
PubMed ID:14632149
'An affinity probe capillary electrophoresis (APCE) assay for guanine-nucleotide-binding proteins (G proteins) was developed using BODIPY FL GTPgammaS (BGTPgammaS), a fluorescently labeled GTP analogue, as the affinity probe. In the assay, BGTPgammaS was incubated with samples containing G proteins and the resulting mixtures of BGTPgammaS-G protein complexes and free BGTPgammaS
Designed FHIT alleles establish that Fhit-induced apoptosis in cancer cells is limited by substrate binding.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12574506
'The FHIT gene is inactivated early in the development of many human tumors, and Fhit-deficient mice have increased cancer incidence. Viral reexpression of Fhit kills Fhit-deficient cells by induction of apoptosis. Fhit, a member of branch 2 of the histidine-triad superfamily of nucleoside monophosphate hydrolases and transferases, is a diadenosine
Fhit-nucleotide specificity probed with novel fluorescent and fluorogenic substrates.
Journal:J Biol Chem
PubMed ID:10671479
'Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins, binds and cleaves diadenosine polyphosphates and functions as a tumor suppressor in human epithelial cancers. Function of Fhit in tumor suppression does not require diadenosine polyphosphate cleavage but correlates with the ability to form substrate complexes. As diadenosine polyphosphates
Mechanism of regulation of prokaryotic tubulin-like GTPase FtsZ by membrane protein EzrA.
Journal:J Biol Chem
PubMed ID:17043359
'At initiation of cell division, FtsZ, a tubulin-like GTPase, assembles into a so-called Z-ring structure at the site of division. The formation of Z ring is negatively regulated by EzrA, which ensures only one ring at the midcell per cell cycle. The mechanism leading to the negative regulation of Z-ring
Use of a native affinity ligand for the detection of G proteins by capillary isoelectric focusing with laser-induced fluorescence detection.
Journal:Electrophoresis
PubMed ID:15274014
'Affinity probe capillary isoelectric focusing (CIEF) with laser-induced fluorescence was explored for detection of Ras-like G proteins. In the assay, a fluorescent BODIPY FL GTP analogue (BGTPgammaS) and G protein were incubated resulting in formation of BGTPgammaS-G protein complex. Excess BGTPgammaS was separated from BGTPgammaS-G protein complex by CIEF using