Réactif TMRM (indicateur de potentiel de membrane mitochondriale) Image-iT™

Citations et références (5)

Invitrogen™

Réactif TMRM (indicateur de potentiel de membrane mitochondriale) Image-iT™

Les membranes mitochondriales saines maintiennent une différence de potentiel électrique entre l’intérieur et l’extérieur d’un organite, appelée potentiel de laAfficher plus

Référence	Quantité
I34361	5 x 100 μl

Référence I34361

Prix (EUR)

212,65

Online Exclusive

251,00

Économisez 38,35 (15%)

Ajouter au panier

Quantité:

5 x 100 μl

Prix (EUR)

212,65

Online Exclusive

251,00

Économisez 38,35 (15%)

Ajouter au panier

Les membranes mitochondriales saines maintiennent une différence de potentiel électrique entre l’intérieur et l’extérieur d’un organite, appelée potentiel de la membrane. L’ester méthylique de tétraméthylrhodamine (TMRM) est un colorant perméant aux cellules qui s’accumule dans les mitochondries actives avec des potentiels de la membrane intacts. Si les cellules sont saines et ont des mitochondries qui fonctionnent, le signal sera lumineux. En cas de perte du potentiel de la membrane mitochondriale, l’accumulation de TMRM cessera et le signal sera faible ou disparaîtra. Le signal TMRM peut être détecté par microscopie par fluorescence, cytométrie en flux, tri cellulaire, criblage à haut débit et analyse à haute teneur.

• Pour l’étude dynamique à cellules vivantes du potentiel de la membrane mitochondriale (non fixable)
• Détectable avec un ensemble de filtres RFP/TRITC (pic d’absorbance : 548 nm, pic d’émission : 574 nm)
• Multiplexable avec des colorants d’émission verts, rouge profond, violets et bleus, tels que GFP, DAPI, Hoechst, HCS LipidTOX et le rouge profond LysoTracker

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Quantité5 x 100 μl

Conditions d’expéditionGlace

CouleurOrange

Gamme de produitsMolecular Probes

Type de produitRéactif

Unit SizeEach

Contenu et stockage

Stocker au congélateur (entre -5°C et -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

For a final staining solution, we can add TMRM directly into cell growth medium. Can this media contain serum?

Yes, you may add TMRM to media with serum. Serum components may bind dye non-specifically so you may need a higher final working concentration for staining samples in media containing serum relative to staining the same cells in a non-serum media or buffer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is TMRM different from the MitoTracker dyes?

TMRM is a dynamic mitochondrial stain. There is no covalent attachment of TMRM to the mitochondria and because of this, it can move in and out of the mitochondria dependent upon changes in the membrane potential. The MitoTracker dyes on the other hand possess a reactive chloromethyl group that allows for their covalent attachment to mitochondria. As a result, once they accumulate within mitochondria that have a normal membrane potential, they are retained.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is included in the Image-iT TMRM Reagent (Cat No. I34361)?

The Image-iT TMRM Reagent is a ready-to-use 1,000X concentrated stock solution of the TMRM dye. It contains 5 vials of 100 µM of TMRM in neat DMSO, with 100 µL volume in each vial. TMRM powder (Cat No. T668) is not included in this kit.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (5)

Citations et références

Abstract

miR-450a Acts as a Tumor Suppressor in Ovarian Cancer by Regulating Energy Metabolism.

Authors:Muys BR, Sousa JF, Plaça JR, de Araújo LF, Sarshad AA, Anastasakis DG, Wang X, Li XL, de Molfetta GA, Ramão A, Lal A, Vidal DO, Hafner M, Silva WA

Journal:Cancer Res

PubMed ID:31101765

'Dysregulation of miRNA expression is associated with multiple diseases, including cancers, in which small RNAs can have either oncogenic or tumor suppressive functions. Here we investigated the potential tumor suppressive function of miR-450a, one of the most significantly downregulated miRNAs in ovarian cancer. RNA-seq analysis of the ovarian cancer cell ... More

Ursodeoxycholic acid protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction through acting on ALDH1L2.

Authors:Yang Y, Liu S, Gao H, Wang P, Zhang Y, Zhang A, Jia Z, Huang S

Journal:Free Radic Biol Med

PubMed ID:32004633

'Mitochondrial dysfunction plays an important role in acute kidney injury (AKI). Thus, the agents improving the mitochondrial function could be beneficial for treating AKI. Ursodeoxycholic acid (UDCA) has been demonstrated to prevent mitochondrial dysfunction under pathology, however, its role in AKI and the underlying mechanism remain unknown. This study aimed ... More

Targeting mitochondria-associated membranes as a potential therapy against endothelial injury induced by hypoxia.

Authors:Yang YD, Li MM, Xu G, Zhang EL, Chen J, Sun B, Chen DW, Gao YQ

Journal:J Cell Biochem

PubMed ID:31241212

Mitochondrial dysfunction plays a principal role in hypoxia-induced endothelial injury, which is involved in hypoxic pulmonary hypertension and ischemic cardiovascular diseases. Recent studies have identified mitochondria-associated membranes (MAMs) that modulate mitochondrial function under a variety of pathophysiological conditions such as high-fat diet-mediated insulin resistance, hypoxia reoxygenation-induced myocardial death, and hypoxia-evoked vascular smooth ... More

Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING Response.

Authors:Ma H, Qian W, Bambouskova M, Collins PL, Porter SI, Byrum AK, Zhang R, Artyomov M, Oltz EM, Mosammaparast N, Miner JJ, Diamond MS

Journal:mBio

PubMed ID:32156810

Although the pathogen recognition receptor pathways that activate cell-intrinsic antiviral responses are well delineated, less is known about how the host regulates this response to prevent sustained signaling and possible immune-mediated damage. Using a genome-wide CRISPR-Cas9 screening approach to identify host factors that modulate interferon-stimulated gene (ISG) expression, we identified ... More

Mitochondrial Pyruvate Carrier 1 Promotes Peripheral T Cell Homeostasis through Metabolic Regulation of Thymic Development.

Authors:Ramstead AG, Wallace JA, Lee SH, Bauer KM, Tang WW, Ekiz HA, Lane TE, Cluntun AA, Bettini ML, Round JL, Rutter J, O'Connell RM

Journal:Cell Rep

PubMed ID:32130894

Metabolic pathways regulate T cell development and function, but many remain understudied. Recently, the mitochondrial pyruvate carrier (MPC) was identified as the transporter that mediates pyruvate entry into mitochondria, promoting pyruvate oxidation. Here we find that deleting Mpc1, an obligate MPC subunit, in the hematopoietic system results in a specific reduction ... More