Citations et références (19)

Invitrogen™

LC3B Antibody Kit for Autophagy

Le kit d’anticorps LC3 pour l’autophagie comprend un anticorps polyclonal de lapin contre la LC3B qui a été validé pourAfficher plus

Référence	Quantité
L10382	1 kit

Référence L10382

Prix (EUR)

588,00

Quantité:

1 kit

Prix (EUR)

588,00

Le kit d’anticorps LC3 pour l’autophagie comprend un anticorps polyclonal de lapin contre la LC3B qui a été validé pour être utilisé en microscopie par fluorescence et en imagerie et analyse à haute teneur. Du diphosphate de chloroquine est également inclus - après traitement avec ce composé, le flux autophagique normal est perturbé et les autophagosomes s’accumulent en raison de la perturbation du flux autophagique normal.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Méthode de détectionFluorescence, Fluorescent

Type de colorantNon marqué

FormatPlaque de 96 puits, lame(s), Plaque à 96 puits, lame(s)

Quantité1 kit

Conditions d’expéditionGlace carbonique

À utiliser avec (équipement)Microscope confocal, microscope à fluorescence, instrument à haute densité, Microscope confocal, Microscope à fluorescence, Instruments d’analyse à haut contenu

Type de produitColorant

Unit SizeEach

Contenu et stockage

Le kit comprend un anticorps polyclonal de lapin contre LC3B et le diphosphate de chloroquine.

Conserver à ≤ -20°C

Foire aux questions (FAQ)

How does the LC3B Antibody Kit for Autophagy work?

The LC3B protein plays a critical role in autophagy. This protein resides in the cytosol, but following cleavage and lipidation with phosphatidylethanolamine, LC3 associates with the phagophore. This localization can be used as a general marker for autophagic membranes. The LC3B Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use in fluorescence microscopy and high content imaging and analysis. The kit also includes chloroquine diphosphate for artificially generating autophagosomes. Following chloroquine diphosphate treatment, lysosomal pH increases and the normal autophagic flux is disrupted, resulting in autophagosome accumulation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (19)

Citations et références

Abstract

WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition.

Authors:Ho H, Kapadia R, Al-Tahan S, Ahmad S, Ganesan AK,

Journal:J Biol Chem

PubMed ID:21317285

'Recent studies implicate a role for WD repeat domain, phosphoinositide-interacting 1 (WIPI1) in the biogenesis of melanosomes, cell type-specific lysosome-related organelles. In this study, we determined that WIPI1, an ATG18 homologue that is shown to localize to both autophagosomes and early endosomes, inhibited mammalian target of rapamycin (MTOR) signaling, leading ... More

Membrane fusion-mediated autophagy induction enhances morbillivirus cell-to-cell spread.

Authors:Delpeut S, Rudd PA, Labonté P, von Messling V,

Journal:J Virol

PubMed ID:22647692

In the context of viral infections, autophagy induction can be beneficial or inhibitory. Within the Paramyxoviridae family, only morbilliviruses have been investigated and are reported to induce autophagy. Here we show that morbilliviruses rapidly induce autophagy and require this induction for efficient cell-to-cell spread. Coexpression of both glycoproteins in cells ... More

Hormonally active vitamin D3 (1alpha,25-dihydroxycholecalciferol) triggers autophagy in human macrophages that inhibits HIV-1 infection.

Authors:Campbell GR, Spector SA,

Journal:J Biol Chem

PubMed ID:21454634

Autophagy is a self-digestion pathway essential for maintaining cellular homeostasis and cell survival and for degrading intracellular pathogens. Human immunodeficiency virus-1 (HIV-1) may utilize autophagy for replication as the autophagy-related protein-7 (ATG-7), microtubule-associated protein 1 light chain 3, ATG-12, and ATG-16L2 are required for productive HIV-1 infection; however, the effects ... More

Galectin-8 Senses Phagosomal Damage and Recruits Selective Autophagy Adapter TAX1BP1 To Control Mycobacterium tuberculosis Infection in Macrophages.

Authors:

Journal:MBio

PubMed ID:34225486

Malpighian Tubule Cells in Overwintering Cave Crickets Troglophilus cavicola (Kollar, 1833) and T. neglectus Krauss, 1879 (Rhaphidophoridae, Ensifera).

Authors:Lipovšek S, Novak T, Janžekovic F, Weiland N, Leitinger G

Journal:PLoS One

PubMed ID:27379687

'During winter, cave cricket larvae undergo dormancy in subterranean habitats; this dormancy is termed diapause in second year Troglophilus cavicola larvae because they mature during this time, and termed quiescence in T. neglectus, because they mature after dormancy. Here we used electron microscopy to analyze ultrastructural changes in the epithelial ... More

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