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Citations et références (702)
Invitrogen™
Lucifer Yellow CH, Lithium Salt, 25 mg
Colorant hydrosoluble avec pics d’excitation / d’émission de 428/536 nm
| Référence | Quantité |
|---|---|
| L453 | 25 mg |
Référence L453
Prix (EUR)
256,65
Online Exclusive
276,00Économisez 19,35 (7%)
Each
Quantité:
25 mg
Prix (EUR)
256,65
Online Exclusive
276,00Économisez 19,35 (7%)
Each
Le Lucifer yellow CH sous forme de sel de lithium est un colorant hydrosoluble avec des pics dʼexcitation/dʼémission de 428/536 nm. Cet outil populaire est communément utilisé pour lʼétude de la morphologie neuronale en raison du groupe de carbohydrazide (CH) quʼil contient et qui lui permet de former une liaison covalente avec les biomolécules environnantes durant la fixation de lʼaldéhyde. Le Lucifer yellow CH sous forme de sel de lithium est fréquemment utilisé pour les micro-injections du fait de sa solubilité supérieure à celle des sels de potassium et dʼammonium du Lucifer yellow CH.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
CouleurJaune
Méthode de détectionFluorescence
À utiliser avec (équipement)Microscope à fluorescence
Poids moléculaire457.24
Type de produitSel de lithium
Quantité25 mg
Conditions d’expéditionTempérature ambiante
Localisation subcellulaireCytoplasme & cytosol
Excitation/Emission428/536 nm
Unit SizeEach
Contenu et stockage
Stocker à température ambiante et à l’abri de la lumière.
Citations et références (702)
Citations et références
Abstract
Endothelial and smooth muscle cell conduction in arterioles controlling blood flow.
Journal:Am J Physiol
PubMed ID:9458866
We performed intracellular recording with Lucifer yellow dye microinjection to investigate the cellular pathway(s) by which constriction and dilation are conducted along the wall of arterioles (diameter 47 +/- 1 microns, n = 63) supplying blood flow to the cheek pouch of anesthetized hamsters. At rest, membrane potential (Em) of
Bursting response to current-evoked depolarization in rat CA1 pyramidal neurons is correlated with lucifer yellow dye coupling but not with the presence of calbindin-D28k.
Journal:Synapse
PubMed ID:2042109
Calbindin-D28k (CaBP) immunohistochemistry has been combined with electrophysiological recording and Lucifer Yellow (LY) cell identification in the CA1 region of the rat hippocampal formation. CaBP is shown to be contained within a distinct sub-population of CA1 pyramidal cells which is equivalent to the superficial layer described by Lorente de Nó
Membrane conductance oscillations in astrocytes induced by phorbol ester.
Journal:Nature
PubMed ID:3627267
Glial cells in the central nervous systems (CNS) have complex functions which are difficult to decipher because of the intimate intertwining of glial cells with neurons. We have therefore developed an essentially neuron-free preparation of CNS astrocytes in the kainic acid lesioned hippocampal slice. With this preparation we have examined
Deformation of network connectivity in the inferior olive of connexin 36-deficient mice is compensated by morphological and electrophysiological changes at the single neuron level.
Journal:J Neurosci
PubMed ID:12805309
Compensatory mechanisms after genetic manipulations have been documented extensively for the nervous system. In many cases, these mechanisms involve genetic regulation at the transcription or expression level of existing isoforms. We report a novel mechanism by which single neurons compensate for changes in network connectivity by retuning their intrinsic electrical
Dual fluorescence combined with a two-color immunoperoxidase technique: a new way of visualizing diverse neuronal elements.
Journal:J Neurosci Methods
PubMed ID:1712061
'A method is described that allows an estimation of the neurotransmitter-related immunoreactivity, morphology and relationship to other immunoreactive elements, of single functionally identified neurons in the central nervous system. First, neurons are identified electrophysiologically using intracellular recording and labelled by iontophoresis of lucifer yellow (LY). After fixation and sectioning of