Citations et références (69)

Invitrogen™

Kit de viabilité du sperme LIVE/DEAD™

Le kit de viabilité du sperme LIVE/DEAD offre un nouveau dosage basé sur la fluorescence pour l’analyse de la viabilitéAfficher plus

Référence	Quantité
L7011	1 kit

Référence L7011

Prix (EUR)

934,00

Quantité:

1 kit

Prix (EUR)

934,00

Le kit de viabilité du sperme LIVE/DEAD offre un nouveau dosage basé sur la fluorescence pour l’analyse de la viabilité et du potentiel de fertilité du sperme par microscopie par fluorescence ou cytométrie de flux. Le colorant d’acide nucléique SYBR® 14 perméant aux cellules marque le sperme vivant avec une fluorescence verte, et de l’iodure de propidium imperméant aux cellules marque les acides nucléiques du sperme dont la membrane est altérée avec une fluorescence rouge.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Type de celluleCellules eucaryotes, spermatozoïdes

DescriptionKit de viabilité du sperme LIVE/DEAD™

Méthode de détectionFluorescence, Fluorescent

Type de colorantAutre(s) étiquette(s) ou colorant(s)

FormatTube(s), lame(s), Tube(s), lame(s)

Quantité1 kit

Conditions d’expéditionTempérature ambiante

CouleurVert, rouge

Emission516, 617 nm

À utiliser avec (application)Test de viabilité

À utiliser avec (équipement)Microscope à fluorescence, cytomètre en flux, Microscope à fluorescence, Cytomètre en flux

Gamme de produitsLIVE/DEAD

Type de produitKit de viabilité de sperme

Unit SizeEach

Contenu et stockage

Conserver au congélateur (entre -5°C et -30°C) et à l’abri de la lumière.

Foire aux questions (FAQ)

What is the fluorescence emission maxima of SYBR 14 dye and propidium iodide?

When bound to DNA, the fluorescence emission maxima of these dyes are 516 nm and 617 nm, respectively. The spectra are available in Figure 1 of the Product Manual.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (69)

Citations et références

Abstract

Seminal plasma addition attenuates the dilution effect in bovine sperm.

Authors:Garner DL,Thomas CA,Gravance CG,Marshall CE,DeJarnette JM,Allen CH

Journal:Theriogenology

PubMed ID:11467516

Dilution of semen to low cell numbers/dose can result in a bull-dependent reduction in the post-thaw viability of cryopreserved bovine spermatozoa. It is possible that essential seminal plasma components are lacking at the greater dilution rates, thereby contributing to the deleterious effects of semen dilution. Ejaculates of 6 Holstein bulls ... More

Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation.

Authors:Thurston LM, Siggins K, Mileham AJ, Watson PF, Holt WV

Journal:Biol Reprod

PubMed ID:11870056

This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) molecular markers linked to genes controlling semen freezability can be identified using amplified restriction fragment length polymorphism (AFLP) technology. Five ejaculates were collected from each of 129 boars. Semen ... More

Comparison of assessment of fowl sperm viability by eosin-nigrosin and dual fluorescence (SYBR-14/PI).

Authors:Chalah T, Brillard JP

Journal:Theriogenology

PubMed ID:10732141

'The kinetics of fowl sperm viability/mortality following short-term and long-term in vitro storage were studied using 2 different staining methods: eosin/nigrosin (observed under light microscopy) and SYBR-14/PI (dual fluorescence). Based on data obtained at 0, 30 min and at 2, 4 and 24 h (T0, T30, T2, T4, and T24) ... More

Seasonal changes of semen quality and freezability in Franches-Montagnes stallions.

Authors:Janett F, Thun R, Bettschen S, Burger D, Hassig M

Journal:Anim Reprod Sci

PubMed ID:12695055

'The objective of this study was to investigate seasonal changes of semen quality parameters in Franches-Montagnes stallions and to compare the freezability of ejaculates collected in autumn and winter. Experiments were performed using 15 stallions from the National Stud Farm in Avenches (Switzerland). Ejaculates were collected and evaluated every month ... More

Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote.

Authors:Kamau SW, Hurtado M, Müller-Doblies UU, Grimm F, Nunez R

Journal:Cytometry

PubMed ID:10918286

'BACKGROUND: Leishmaniasis is a major tropical and subtropical parasitic disease. Sodium stibogluconate, N-methyl -D-glucamine antimoniate, amphotericin B, pentamidine, and ketoconazole are drugs used to treat this disease. Some of these drugs cause severe adverse side effects and treatment failures are common. Allopurinol, a purine analog, has been used to treat ... More

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