2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-désoxyglucose)
2-NBDG (2-(<i>N</i>-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-désoxyglucose)
Citations et références (39)
Invitrogen™

2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-désoxyglucose)

Le 2-NBDG est un analogue fluorescent du glucose qui permet de surveiller l’absorption du glucose dans les cellules vivantes. IlAfficher plus
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RéférenceQuantité
N131955 mg
Référence N13195
Prix (EUR)
319,65
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346,00
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Quantité:
5 mg
Prix (EUR)
319,65
Online Exclusive
346,00
Économisez 26,35 (8%)
Each
Ajouter au panier
Le 2-NBDG est un analogue fluorescent du glucose qui permet de surveiller l’absorption du glucose dans les cellules vivantes. Il indique la viabilité cellulaire. Bien que sensible à son environnement, la fluorescence NBD affiche généralement des valeurs maximales d’excitation/émission d’environ 465/540 nm. Elle peut être visualisée avec des filtres optiques conçus pour la fluorescéine.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Méthode de détectionFluorescence
Type de colorantAutre(s) étiquette(s) ou colorant(s)
FormatTube(s), lame(s)
Quantité5 mg
Conditions d’expéditionTempérature ambiante
À utiliser avec (équipement)Microscope à fluorescence, Cytomètre en flux
Type de produit2-NBDG
Unit SizeEach
Contenu et stockage
Stocker au congélateur (entre -5°C et -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

What are the final concentrations of 2-NBDG and 6-NBDG, and incubation times that may be used?

Final concentrations of 2-NBDG can range from 10 µM to 600 µM; 100 µM to 600 µM have been used with bacteria and yeasts and, from 10 µM to 200 µM for primary and cultured mammalian cells.

Final concentrations of 6-NBDG may range from 30 µM up to 300 µM.

Cells should be incubated at the desired temperature and time to allow for sufficient detection of green-yellow fluorescence within cells. Uptake is temperature dependent. Incubation times may range from seconds up to 30 minutes or longer, dependent upon the final concentration of the reagent, cell type, culture conditions, and other factors.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What solvents can be used to resolubilize either 2-NBDG or 6-NBDG to make a stock solution, and how should this stock solution be stored?

Compatible solvents (and approximate maximum solubility): DMSO ( ˜10 mg/mL), DMF (˜10 mg/mL), ethanol (˜20 mg/mL), methanol (˜20 mg/mL), H2O (˜10 mg/mL), PBS, pH 7.2 (˜10 mg/mL).

Stock solutions may be prepared using the recommended solvents in concentrations at or below their maximum solubility and then stored frozen, desiccated (for non-aqueous solvents), and protected from light. Aqueous solutions should not be stored longer than one day.

For long-term storage, these reagents should be stored as a solid at ≤–20°C. To make smaller aliquots, dissolve the reagent in ethanol, make smaller aliquots in separate vials and then evaporate the solvent using a vacuum pump. Do not use DMSO or DMF due to their low vapor pressure.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (39)

Citations et références
Abstract
Measurement of glucose uptake and intracellular calcium concentration in single, living pancreatic beta-cells.
Authors:Yamada K, Nakata M, Horimoto N, Saito M, Matsuoka H, Inagaki N
Journal:J Biol Chem
PubMed ID:10748091
'There has been no method previously to measure both glucose transport and its effect on the various intracellular functions in single, living mammalian cells. A fluorescent derivative of d-glucose, 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxy-d-glucose (2-NBDG), that we have developed has made such measurements possible. COS-1 cells that overexpress the human glucose transporter GLUT2 ... More
Glutamate triggers rapid glucose transport stimulation in astrocytes as evidenced by real-time confocal microscopy.
Authors:Loaiza A, Porras OH, Barros LF
Journal:J Neurosci
PubMed ID:12917367
'Glutamate stimulates glycolysis in astrocytes, a phenomenon that couples astrocytic metabolism with neuronal activity. However, it is not known whether glutamate also affects glucose transporter-1 (GLUT1), the transporter responsible for glucose entry into astrocytes. To address this question, two different real-time single-cell hexose uptake assays were applied to cultured hippocampal ... More
Fluorescent microplate cell assay to measure uptake and metabolism of glucose in normal human lung fibroblasts.
Authors:Leira F, Louzao MC, Vieites JM, Botana LM, Vieytes MR
Journal:Toxicol In Vitro
PubMed ID:12020600
'This is the first report of a fluorimetric microplate assay to assess glucose uptake and metabolism in eukaryotic cells. The assay was carried out incubating normal human lung fibroblasts in the wells of microtiter trays with a fluorescent D-glucose derivative, 2-N-7-(nitrobenz-2-oxa-1,3-diazol-4-yl)amino-2-deoxy-D-glucose (2-NBDG). This dye could be incorporated by glucose transporting ... More
Rapid viability assessment of yeast cells using vital staining with 2-NBDG, a fluorescent derivative of glucose.
Authors:Oh KB, Matsuoka H
Journal:Int J Food Microbiol
PubMed ID:12038577
'A fluorescent glucose analogue, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG), which had been developed previously for the analysis of glucose uptake activity by living cells, was investigated to evaluate its applicability for assaying the viability of yeasts. Fluorescence intensities of the yeast population were measured by fluorescence spectrophotometry upon exposure to antifungal agents ... More
Intracellular fate of 2-NBDG, a fluorescent probe for glucose uptake activity, in Escherichia coli cells.
Authors:Yoshioka K, Saito M, Oh KB, Nemoto Y, Matsuoka H, Natsume M, Abe H
Journal:Biosci Biotechnol Biochem
PubMed ID:8987871
'A fluorescent derivative of D-glucose, 2-NBDG, which was previously developed for the evaluation of glucose uptake activity by living cells, was used on Escherichia coli cells and its fate after incorporation in the cells was investigated. 2-NBDG was converted to another fluorescent derivative (2-NBDG metabolite) immediately after it was taken ... More
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