DryEase™ Mini-Gel Drying System

Le Système de séchage de mini-gel DryEase® est un kit simple et facile à utiliser pour le séchage uniforme desAfficher plus

Référence	Quantité
NI2387	1 système

Référence NI2387

Prix (EUR)

366,00

Ajouter au panier

Quantité:

1 système

Prix (EUR)

366,00

Ajouter au panier

Le Système de séchage de mini-gel DryEase® est un kit simple et facile à utiliser pour le séchage uniforme des gels grâce à l’évaporation passive. Le kit permet aux gels d’être montés sur une base située entre deux cadres ouverts de sorte que les deux surfaces extérieures des gels soient exposées à l’air. Par conséquent, le séchage est uniforme et le craquèlement minimisé. La solution de séchage exclusive Gel-Dry™ module le taux de libération de l’eau afin de garantir des gels sans craquelures. Avec le système DryEase®, les gels sont séchés pendant la nuit avec une tension uniforme sur tous les points du gel afin d’éliminer le craquèlement. Tous les composants du système sont également disponibles à l’achat séparément.

Applications : Les gels séchés avec le Système DryEase® sont adaptés à la fluorographie, la densitométrie, l’autoradiographie et au stockage permanent du gel.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Gamme de produitsDryEase, Novex

Type de produitMini système de séchage de gel

Quantité1 système

Durée de conservation1 an

À utiliser avec (équipement)Système de séchage de mini gel DryEase™

Unit SizeEach

Contenu et stockage

Le Kit de séchage de mini-gel DryEase™ contient 2 Cadres de séchage de mini-gel DryEase™ avec 8 Pinces Gel-Dry™, une Base de séchage de mini-gel DryEase™, un Mini cellophane DryEase™ (200 feuilles prédécoupées) et une Solution de séchage Gel-Dry™ (500 ml). Stocker la solution à température ambiante. Stabilité garantie 1 an sous réserve de respecter les conditions de conservation.

Foire aux questions (FAQ)

I dried my gel using your DryEase Mini Gel Drying system and the gel cracked during drying. Can you please help?

Please see below for possible causes and solutions:

- Air trapped between cellophane layers. Apply adequate Gel-Drying solution to prevent bubbles between the gel and cellophane assembly.
- Tension on cellophane too strong. Take care not to stretch the cellophane during dryer assembly.
- Rough edges or small cracks on gel edges. Trim rough edges from the gel to eliminate starting points for cracks.
- Cellophane slips during drying. Make sure clamps hold all four edges of the dryer assembly firmly.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I am using your DryEase Mini Gel Drying system and would like to peel off the cellophane after drying the gel but it seems really difficult. Can you please offer some tips?

Usually it is not necessary to peel off the cellophane. If your signal is very faint and you are concerned about getting an exposure, you can rub the top surface of your gel with a damp towel and then peel off the cellophane. Then air dry the gel for a few minutes and perform autoradiography.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

I dried my gel using Gel-Drying Solution and it dried milky white. Can the gel be recovered?

Gels turn white when they are dried too rapidly, usually in an environment that is too ventilated, drafty, or warm. You can remedy this problem by dabbing the gel with deionized water on a Kimwipe tissue to rehydrate the white areas. Re-dry the gel in a dark, dry, non-drafty place, such as a drawer or cabinet.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the pore size of the DryEase cellophane?

The pore size of DryEase cellophane is 40 microns.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Do you offer a system for drying of protein gels?

We offer the following systems for drying of protein gels:

DryEase Mini Gel Drying System (Cat. No. NI2387): Simple-to-use kit for even, crack-free drying of mini gels that dries gels evenly, using passive evaporation. Gels dried with this system are suitable for fluorography, densitometry, autoradiography, or permanent gel storage. Each DryEase Mini-Gel Drying System contains the following components:

- 2-DryEase Mini-Gel Drying Frames (includes 2 frames + 8 clamps) (Cat. No. NI2380)
- 1-DryEase Mini-Gel Drying Base (Cat. No. NI2300)
- 200-DryEase Mini Cellophane precut sheets (Cat. No. NC2380)
- 500 mL Gel-Dry Drying Solution (Cat. No. NI2100)
Large Gel Drying Kit (Cat. No. NI2207): Provides the same even, crack-free gel drying seen with the DryEase Mini-Gel Drying System but is designed to accommodate larger gels or 4 mini-gels. A base for the system is not required.

Each Large Gel Drying Kit contains the following components:

- 1-DryEase Large Gel Drying Frame (includes 8 clamps) (Cat. No. NI2200)
- 100-DryEase Large Cellophane precut sheets (Cat. No. NC2200)
- 500 mL Gel-Dry Drying Solution (Cat. No. LC4025)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Citations et références (1)

Citations et références

Abstract

In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins.

Authors:Kaufman BA, Newman SM, Hallberg RL, Slaughter CA, Perlman PS, Butow RA

Journal:Proc Natl Acad Sci U S A

PubMed ID:10869431

The segregating unit of mtDNA is a protein-DNA complex called the nucleoid. In an effort to understand how nucleoid proteins contribute to mtDNA organization and inheritance, we have developed an in organello formaldehyde crosslinking procedure to identify proteins associated with mtDNA. Using highly purified mitochondria, we observed a time-dependent crosslinking ... More