Invitrogen™

NuPAGE™ Bis-Tris Welcome Pack, 12%

Name: NuPAGE™ Bis-Tris Welcome Pack, 12%
SKU: NP034C
Price: 886.00 EUR

Le Welcome Pack Bis-Tris NuPAGE contient tous les gels, tampons et réactifs NuPAGE dont vous avez besoin pour commencer à utiliser la mini cuve à gel.

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Référence	Puits
NP034C	15 puits
NP034A	10 puits
NP034B	12 puits

3 options

Référence NP034C

Prix (EUR)

886,00

Each

Puits:

15 puits

Prix (EUR)

886,00

Each

Les packs de bienvenue Bis-Tris NuPAGE contiennent tous les gels, tampons et réactifs NuPAGE dont vous avez besoin pour commencer à utiliser la mini cuve à gel. La mini cuve à gel est compatible avec tous les mini gels Invitrogen Novex, NuPAGE et Bolt. Chaque mini cuve à gel peut accueillir jusqu’à deux gels par cycle. La conception unique de la cuve favorise le chargement pratique des gels côte à côte et une meilleure visualisation pendant l’utilisation. Les durées de cycle peuvent varier selon les conditions de tampon et les alimentations utilisées.

Le pack de bienvenue NuPAGE Bis-Tris contient :
• Cuve à gel mini (A25977)
• Gels mini NuPAGE Bis-Tris (2 boîtes, 20 gels)
• Tampon de migration NuPAGE MES SDS, 20X (NP0002)
• Tampon d’échantillon NuPAGE LDS, 4X (NP0007)
• Agent de réduction d’échantillons NuPAGE, 10X (NP0009)
• Échelle de protéines précolorées PageRuler Plus, 10 à 250 kDa (26619)

À propos de la cuve à gel mini
La cuve à gel mini est compatible avec tous les gels mini Invitrogen Novex, NuPAGE et Bolt. Chaque Mini Gel Tank peut accueillir jusqu’à deux gels par cycle. La conception unique de la cuve favorise le chargement pratique des gels côte à côte et une meilleure visualisation pendant l’utilisation. La durée d’un cycle peut varier en fonction de l’état d’un tampon et de la source d’alimentation utilisée.

À propos des gels NuPAGE Bis-Tris
Les gels de protéines NuPAGE Bis-Tris Invitrogen sont des gels de polyacrylamide prémoulés conçus pour une séparation optimale d’une vaste gamme de protéines dans des conditions dénaturantes. Contrairement aux gels traditionnels Tris-Glycine, les gels NuPAGE Bis-Tris ont un environnement à pH neutre qui minimise la modification des protéines. Utilisez les gels NuPAGE Bis-Tris pour préparer les protéines au séquençage, à la spectrométrie de masse et à toutes les autres techniques dans lesquelles l’intégrité des protéines est cruciale. Vous pouvez également utiliser les gels NuPAGE pour obtenir des résultats quotidiens optimaux.

Transfert
Pour transférer vos protéines vers une membrane, nous recommandons d’utiliser le tampon de transfert NuPAGE (NP0006) ou, pour un transfert humide traditionnel, le mini module de transfert (B1000). Alternativement, vous pouvez effectuer un transfert rapide semi-sec à l’aide du buvard d’alimentation Invitrogen ou un transfert rapide sec avec le dispositif de transfert de gel iBlot 2 (IB21001).

For Research Use Only. Not for use in diagnostic procedures.

Spécifications

À utiliser avec (équipement)Mini-cuve à gel Mini Gel Tank

Gel Thickness1,0 mm

Quantité1 Welcome Pack

Conditions d’expéditionHomologué pour l’expédition à température ambiante et sur glace humide

Pourcentage de gel12 %

Dimensions de gelMini

Type de gelBis-Tris

Gamme de produitsNuPAGE

Plage de séparation3,5 à 80 kDa

Puits15 puits

Unit SizeEach

Foire aux questions (FAQ)

I used one of your protein standards for a western transfer and noticed that some of the lower-molecular weight protein bands passed through the membrane. How can I resolve this issue?

- Decrease voltage, current or length of transfer time
- Make sure that the methanol concentration in the transfer buffer is proper; use a methanol concentration of 10-20% methanol removes the SDS from SDS-protein complexes and improves the binding of protein to the membrane.
- Make sure that the SDS concentration (if added) in the transfer buffer is proper, don't use more than 0.02-0.04% SDS. Using too much SDS can prevent binding of proteins to the membrane.
- Check the pore size of the membrane and the size of the target protein. Proteins smaller than 10 kDa will easily pass through a 0.45 µm pore size membrane. If proteins smaller than 10 kDa are of interest, it would be better to use a 0.2 µm pore size membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used one of your protein standards for a western transfer and noticed that some of the higher-molecular weight bands transferred very poorly to the membrane. Can you offer some tips?

- Increase voltage, current or length of time for transfer
- SDS in the gel and in the SDS-protein complexes promotes elution of the protein from the gels but inhibits binding of the protein to membranes. This inhibition is higher for nitrocellulose than for PVDF. For proteins that are difficult to elute from the gel such as large molecular weight proteins, a small amount of SDS may be added to the transfer buffer to improve transfer. We recommend pre-equilibrating the gel in 2X Transfer buffer (without methanol) containing 0.02-0.04% SDS for 10 minutes before assembling the sandwich and then transferring using 1X transfer buffer containing 10% methanol and 0.01%SDS.
- Methanol removes the SDS from SDS-protein complexes and improves the binding of protein to the membrane, but has some negative effects on the gel itself, leading to a decrease in transfer efficiency. It may cause a reduction in pore size, precipitation of some proteins, and some basic proteins to become positively charged or neutral. Make sure that the methanol concentration in the transfer buffer is not more than 10-20% and that high-quality, analytical grade methanol is used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used one of your pre-stained standards on a Tris-Glycine gel and noticed that the molecular weights of the proteins were different than on a NuPAGE Bis-Tris gel. What is the reason for this?

Pre-stained standards have a dye that is covalently bound to each protein that will result in the standard migrating differently in different buffer systems (i.e., different gels). As a result, using a pre-stained standard for molecular weight estimation will only give the apparent molecular weight of the protein. Pre-stained standards may be used for molecular weight approximation, confirming gel migration and estimating blotting efficiency but for accurate molecular weight estimation, an unstained standard should be used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used one of your protein standards and am seeing some extra bands in the lane. Can you offer some suggestions?

- While loading, take care to make sure that there is no cross-contamination from adjacent sample lanes.
- Make sure that the correct amount of standard is loaded per lane. Loading too much protein can result in extra bands and this is a problem especially with silver-stained gels.
- Improper storage of the standard or repeated freeze/thawing can result in protein degradation.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used one of your protein standards and the bands look non-distinct and smeary. What should I do?

Here are some suggestions:

- Make sure that the correct amount of standard is loaded per lane. Loading too much protein can cause smearing and this is a problem especially with silver stained gels.
- Bands will not be as well resolved in low percentage gels. Try using a higher percentage gel.
- If the bands look smeary and non-distinct after a western transfer/detection, this may be due to the antibody being too concentrated. Follow the manufacturer's recommended dilution or determine the optimal antibody concentration by dot-blotting.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all NuPAGE™ Bis-Tris Welcome Pack, 12% FAQs