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Citations et références (29)
Invitrogen™
Paclitaxel, Oregon Green™ 488 Conjugate (Oregon Green™ 488 Taxol, Flutax-2)
Ce dérivé du paclitaxel Oregon Green 488 s’avère être une sonde importante pour le marquage des filaments de tubuline dansAfficher plus
| Référence | Quantité |
|---|---|
| P22310 | 100 μg |
Référence P22310
Prix (EUR)
788,00
Each
Quantité:
100 μg
Prix (EUR)
788,00
Each
Ce dérivé du paclitaxel Oregon Green 488 s’avère être une sonde importante pour le marquage des filaments de tubuline dans les cellules vivantes. L’étiquette fluorescente de cette nouvelle sonde est fixée au 7-carbone du paclitaxel, une stratégie permettant la liaison sélective de la sonde aux microtubules. Les chercheurs ont utilisé des dérivés de paclitaxel fluorescents similaires dans divers systèmes.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Type de colorantOregon Green™ 488
Gamme de produitsOregon Green
Quantité100 μg
Conditions d’expéditionTempérature ambiante
Type d’étiquetteColorants classiques
Type de produitPaclitaxel
SubCellular LocalizationTubuline, cytosquelette, Tubulin
Unit SizeEach
Contenu et stockage
Stocker au congélateur (entre -5°C et -30°C) à l’abri de la lumière.
Citations et références (29)
Citations et références
Abstract
The interaction of baccatin III with the taxol binding site of microtubules determined by a homogeneous assay with fluorescent taxoid.
Journal:Biochemistry
PubMed ID:11580273
'The ubiquitous Taxol binding site of microtubules also binds newly discovered ligands. We have designed a homogeneous assay for the high throughput detection of Taxol biomimetics, based on the displacement of 7-O-[N-(2,7-difluoro-4''-fluoresceincarbonyl)-L-alanyl]Taxol from its binding site in diluted solutions of preserved microtubules. The state of this reference ligand is measured
An incremental approach to automated protein localisation.
Journal:BMC Bioinformatics
PubMed ID:18937856
'BACKGROUND: The subcellular localisation of proteins in intact living cells is an important means for gaining information about protein functions. Even dynamic processes can be captured, which can barely be predicted based on amino acid sequences. Besides increasing our knowledge about intracellular processes, this information facilitates the development of innovative
Molecular recognition of taxol by microtubules. Kinetics and thermodynamics of binding of fluorescent taxol derivatives to an exposed site.
Journal:J Biol Chem
PubMed ID:10818101
'We have determined the kinetic scheme and the reaction rates of binding to microtubules of two fluorescent taxoids, 7-O-[N-(4''-fluoresceincarbonyl)-l-alanyl]Taxol (Flutax-1) and 7-O-[N-(2,7-difluoro-4''-fluoresceincarbonyl)-l-alanyl]Taxol (Flutax-2). Flutax-1 and Flutax-2 bind to microtubules with high affinity (K(a) approximately 10(7) m(-1), 37 degrees C). The binding mechanism consists of a fast bimolecular reaction followed by
Fluorescent taxoid probes for microtubule research.
Journal:Methods Cell Biol
PubMed ID:20466144
'The use of the antitumor drug taxol as an experimental microtubule-stabilizing agent is widespread. Fluorescent taxol conjugates, although less employed, are very useful tools for several purposes in microtubule research. These include easily visualizing microtubule cytoskeletons in a variety of cells as well as in vitro assembled microtubules, studying the
Perspective of a new diagnostic for human trichomonosis.
Journal:Mem Inst Oswaldo Cruz
PubMed ID:12764446
Several diagnostic techniques have been employed for the detection of Trichomonas vaginalis. Microtubules constitute the cytoskeleton in eukaryotic cells and are sensitive to antimitotic drugs, such as Taxol (paclitaxel). We used FLUTAX a fluorescent taxoid - to analyze the microtubule distribution in living trophozoites of T. vaginalis in urine and