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Fluorescein-Ubiquitin
Citations et références (1)

Fluorescein-Ubiquitin

Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due toAfficher plus
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RéférenceQuantité
PV437750 μg
PV4378500 μg
Référence PV4377
Prix (EUR)
385,00
Each
Quantité:
50 μg
Prix (EUR)
385,00
Each
Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.

For use in combination with FRET donors including LanthaScreen™ Tb-Ubiquitin (PV4375) or a LanthaScreen™ antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).
For Research Use Only. Not for use in diagnostic procedures.
Spécifications
Méthode de détectionFluorescence
Excitation / émission340⁄490, 520
À utiliser avec (équipement)Microplate Reader
Type d’étiquetteClassic Dyes
Marqueur ou colorantFITC (Fluorescein)
Quantité50 μg
AffichageEnd Point
Conditions d’expéditionDry Ice
Propriétés du substratProtein-Based Substrate
TechniqueTR-FRET
ConjuguéFluorescein
FormeLiquid
Unit SizeEach
Contenu et stockage
Fluorescein-labeled ubiquitin in HEPES buffered saline. Store at -20°C.

Citations et références (1)

Citations et références
Abstract
TR-FRET-based high-throughput screening assay for identification of UBC13 inhibitors.
Authors:Madiraju C, Welsh K, Cuddy MP, Godoi PH, Pass I, Ngo T, Vasile S, Sergienko EA, Diaz P, Matsuzawa S, Reed JC,
Journal:J Biomol Screen
PubMed ID:22034497
UBC13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBC13 is required for signaling by a variety of receptors important in immune regulation, ... More