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Invitrogen™
Biotin-Ubiquitin
Biotin-labeled ubiquitin for use as a general research tool, or in high throughput assays to monitor protein ubiquitination.
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| Référence | Quantité |
|---|---|
| PV4379 | 10 μg |
| PV4380 | 100 μg |
Référence PV4379
Prix (EUR)
387,00
Each
Quantité:
10 μg
Prix (EUR)
387,00
Each
Biotin-labeled ubiquitin for use as a general research tool, or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.
For use in combination with other LanthaScreen™ products including LanthaScreen™ Tb-Ubiquitin (Cat. No. PV4375), Fluorescein-Ubiquitin (PV4377), or a LanthaScreen™ antibody such as Tb-anti-biotin (PV4749).
For Research Use Only. Not for use in diagnostic procedures.
Spécifications
Méthode de détectionFluorescence
Excitation / émission340/490, 520 nm
À utiliser avec (équipement)Microplate Reader
Type d’étiquetteBiotin & Other Haptens
Marqueur ou colorantBiotin
Quantité10 μg
AffichageEnd Point
Conditions d’expéditionDry Ice
Propriétés du substratProtein-Based Substrate
TechniqueTR-FRET
ConjuguéBiotin
FormeLiquid
Unit SizeEach
Contenu et stockage
Biotin labeled ubiquitin in HEPES buffered saline. Store at -20°C.
Citations et références (1)
Citations et références
Abstract
TR-FRET-based high-throughput screening assay for identification of UBC13 inhibitors.
Journal:J Biomol Screen
PubMed ID:22034497
UBC13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBC13 is required for signaling by a variety of receptors important in immune regulation,