Kits de dosage d’ADNdb Quant-iT™, haute sensibilité (HS) et large gamme (BR)
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
Kits de dosage d’ADNdb Quant-iT™, haute sensibilité (HS) et large gamme (BR)
Citations et références (32)
Invitrogen™

Kits de dosage d’ADNdb Quant-iT™, haute sensibilité (HS) et large gamme (BR)

Pour une quantification hautement sélective de l’ADN double brin sur l’ARN, les kits de dosage d’ADNdb Quant-iT, haute sensibilité et large plage, produisent des signaux de fluorescence linéaires dans les plages de 0,2-100 ng et 2-1 000 ng d’ADN, respectivement.
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RéférenceDoserPlage de quantification
Q33120Quantification de l’ADNdb, haute sensibilité0,2 à 100 ng
Q33130Quantification de l’ADNdb, large plage4 à 1 000 ng
Référence Q33120
Prix (EUR)
783,65
Online Exclusive
890,00
Économisez 106,35 (12%)
Each
Ajouter au panier
Doser:
Quantification de l’ADNdb, haute sensibilité
Plage de quantification:
0,2 à 100 ng
Prix (EUR)
783,65
Online Exclusive
890,00
Économisez 106,35 (12%)
Each
Ajouter au panier
Effectuez une quantification de l’ADNdb facilement et rapidement grâce aux kits de dosage de l’ADNdb Quant-iT. Le kit de dosage d’ADNdb haute sensibilité Quant-iT et le kit de dosage d’ADNdb large gamme Quant-iT fournissent des réactifs de dosage concentrés, un tampon de dilution et des étalons d’ADN pré-dilués. Ces kits de dosage d’ADN sont hautement sélectifs pour l’ADN double brin par rapport à l’ARN ; de plus, dans les plages d’ADN de 0,2 à 100 ng (pour le kit de dosage d’ADNdb HS) et d’ADN de 4 à 1 000 ng (pour le kit de dosage d’ADNdb BR), le signal de fluorescence est linéaire.
Le kit de dosage de haute sensibilité de l’ADNdb Quant-iT et le kit de dosage d’ADNdb Quant-iT large gamme facilitent la quantification de l’ADN et en assurent la précision. Les kits comprennent un réactif d’essai concentré, un tampon de dilution et des étalons d’ADN pré-dilués. Diluez simplement le réactif à 1:200, chargez 200 µl dans les puits d’une microplaque, ajoutez 1 à 20 µl d’échantillon, mélangez, puis lisez la fluorescence.

Les dosages sont hautement sélectifs pour l’ADN double brin par rapport à l’ARN ; de plus, dans les plages de 0,2 à 100 ng pour le dosage HS et de 4 à 1 000 ng pour le dosage BR, le signal de fluorescence est linéaire avec l’ADN. Les dosages sont effectués à température ambiante et le signal est stable pendant 3 heures. Les contaminants courants, tels que les sels, les solvants, les détergents et les protéines, sont bien tolérés dans ces dosages.

For Research Use Only. Not for use in diagnostic procedures.
Spécifications
DoserQuantification de l’ADNdb, haute sensibilité
À utiliser avec (équipement)Lecteur de microplaques
Nbre de réactions1 000 dosages (volume de dosage de 200 μl)
Gamme de produitsQuant-iT
Plage de quantification0,2 à 100 ng
Quantité1 kit
Conditions d’expéditionTempérature ambiante
Méthode de détectionFluorescence
Unit SizeEach

Foire aux questions (FAQ)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

View all Kits de dosage d’ADNdb Quant-iT™, haute sensibilité (HS) et large gamme (BR) FAQs

Citations et références (32)

Citations et références
Abstract
Natural transformation of Myxococcus xanthus.
Authors:Wang J, Hu W, Lux R, He X, Li Y, Shi W,
Journal:J Bacteriol
PubMed ID:21378184
'Myxococcus xanthus belongs to the delta class of the proteobacteria and is notable for its complex life-style with social behaviors and relatively large genome. Although previous observations have suggested the existence of horizontal gene transfer in M. xanthus, its ability to take up exogenous DNA via natural transformation has not ... More
Gastroenteritis outbreak caused by waterborne norovirus at a New Zealand ski resort.
Authors:Hewitt J, Bell D, Simmons GC, Rivera-Aban M, Wolf S, Greening GE,
Journal:Appl Environ Microbiol
PubMed ID:17965205
'In July 2006, public health services investigated an outbreak of acute gastroenteritis among staff and visitors of a popular ski resort in southern New Zealand. The source of the outbreak was a drinking water supply contaminated by human sewage. The virological component of the investigation played a major role in ... More
Expression/localization patterns of sirtuins (SIRT1, SIRT2, and SIRT7) during progression of cervical cancer and effects of sirtuin inhibitors on growth of cervical cancer cells.
Authors:Singh S, Kumar PU, Thakur S, Kiran S, Sen B, Sharma S, Rao VV, Poongothai AR, Ramakrishna G
Journal:
PubMed ID:25794641
'Sirtuins belong to the family of class III histone deacetylases; its role in neoplasia is controversial as both tumor-suppressive and promoting functions have been reported. There are very few reports available, where expressions of sirtuin isoforms are comprehensively analyzed during neoplasia. Therefore, in the present study, the expression of SIRT1, ... More
The altered landscape of the human skin microbiome in patients with primary immunodeficiencies.
Authors:Oh J, Freeman AF, Park M, Sokolic R, Candotti F, Holland SM, Segre JA, Kong HH,
Journal:
PubMed ID:24170601
'While landmark studies have shown that microbiota activate and educate host immunity, how immune systems shape microbiomes and contribute to disease is incompletely characterized. Primary immunodeficiency (PID) patients suffer recurrent microbial infections, providing a unique opportunity to address this issue. To investigate the potential influence of host immunity on the ... More
Effect of the metabolic environment at key stages of follicle development in cattle: focus on steroid biosynthesis.
Authors:Walsh SW, Mehta JP, McGettigan PA, Browne JA, Forde N, Alibrahim RM, Mulligan FJ, Loftus B, Crowe MA, Matthews D, Diskin M, Mihm M, Evans AC,
Journal:Physiol Genomics
PubMed ID:22414914
'Cellular mechanisms that contribute to low estradiol concentrations produced by the preovulatory ovarian follicle in cattle with a compromised metabolic status are largely unknown. To gain insight into the main metabolic mechanisms affecting preovulatory follicle function, two different animal models were used. Experiment 1 compared Holstein-Friesian nonlactating heifers (n = ... More
View all Kits de dosage d’ADNdb Quant-iT™, haute sensibilité (HS) et large gamme (BR) citations