Kits de dosage d’ADNdb Quant-iT™, haute sensibilité (HS) et large gamme (BR)
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
Upgrade to the Quant-iT 1X dsDNA BR Assay Kit with premixed reagent and buffer for added convenience and efficiency
Kits de dosage d’ADNdb Quant-iT™, haute sensibilité (HS) et large gamme (BR)
Invitrogen™

Kits de dosage d’ADNdb Quant-iT™, haute sensibilité (HS) et large gamme (BR)

Pour une quantification hautement sélective de l’ADN double brin sur l’ARN, les kits de dosage d’ADNdb Quant-iT, haute sensibilité et large plage, produisent des signaux de fluorescence linéaires dans les plages de 0,2-100 ng et 2-1 000 ng d’ADN, respectivement.
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RéférenceDoserPlage de quantification
Q33130Quantification de l’ADNdb, large plage4 à 1 000 ng
Q33120Quantification de l’ADNdb, haute sensibilité0,2 à 100 ng
Référence Q33130
Prix (EUR)
822,65
Online Exclusive
924,00
Économisez 101,35 (11%)
Each
Ajouter au panier
Doser:
Quantification de l’ADNdb, large plage
Plage de quantification:
4 à 1 000 ng
Prix (EUR)
822,65
Online Exclusive
924,00
Économisez 101,35 (11%)
Each
Ajouter au panier
Effectuez une quantification de l’ADNdb facilement et rapidement grâce aux kits de dosage de l’ADNdb Quant-iT. Le kit de dosage d’ADNdb haute sensibilité Quant-iT et le kit de dosage d’ADNdb large gamme Quant-iT fournissent des réactifs de dosage concentrés, un tampon de dilution et des étalons d’ADN pré-dilués. Ces kits de dosage d’ADN sont hautement sélectifs pour l’ADN double brin par rapport à l’ARN ; de plus, dans les plages d’ADN de 0,2 à 100 ng (pour le kit de dosage d’ADNdb HS) et d’ADN de 4 à 1 000 ng (pour le kit de dosage d’ADNdb BR), le signal de fluorescence est linéaire.
Le kit de dosage de haute sensibilité de l’ADNdb Quant-iT et le kit de dosage d’ADNdb Quant-iT large gamme facilitent la quantification de l’ADN et en assurent la précision. Les kits comprennent un réactif d’essai concentré, un tampon de dilution et des étalons d’ADN pré-dilués. Diluez simplement le réactif à 1:200, chargez 200 µl dans les puits d’une microplaque, ajoutez 1 à 20 µl d’échantillon, mélangez, puis lisez la fluorescence.

Les dosages sont hautement sélectifs pour l’ADN double brin par rapport à l’ARN ; de plus, dans les plages de 0,2 à 100 ng pour le dosage HS et de 4 à 1 000 ng pour le dosage BR, le signal de fluorescence est linéaire avec l’ADN. Les dosages sont effectués à température ambiante et le signal est stable pendant 3 heures. Les contaminants courants, tels que les sels, les solvants, les détergents et les protéines, sont bien tolérés dans ces dosages.

For Research Use Only. Not for use in diagnostic procedures.
Spécifications
DoserQuantification de l’ADNdb, large plage
À utiliser avec (équipement)Lecteur de microplaques
Nbre de réactions1 000 dosages (volume de dosage de 200 μl)
Gamme de produitsQuant-iT
Plage de quantification4 à 1 000 ng
Quantité1 kit
Conditions d’expéditionTempérature ambiante
Méthode de détectionFluorescence
Unit SizeEach

Foire aux questions (FAQ)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

Citations et références (16)

Citations et références
Abstract
3D chromatin conformation correlates with replication timing and is conserved in resting cells.
Authors:Moindrot B, Audit B, Klous P, Baker A, Thermes C, de Laat W, Bouvet P, Mongelard F, Arneodo A,
Journal:Nucleic Acids Res
PubMed ID:22879376
'Although chromatin folding is known to be of functional importance to control the gene expression program, less is known regarding its interplay with DNA replication. Here, using Circular Chromatin Conformation Capture combined with high-throughput sequencing, we identified megabase-sized self-interacting domains in the nucleus of a human lymphoblastoid cell line, as ... More
A significant effect of the TSPY1 copy number on spermatogenesis efficiency and the phenotypic expression of the gr/gr deletion.
Authors:Shen Y, Yan Y, Liu Y, Zhang S, Yang D, Zhang P, Li L, Wang Y, Ma Y, Tao D, Yang Y,
Journal:Hum Mol Genet
PubMed ID:23307928
'AZFc deletions cause a significant phenotypic heterogeneity with respect to spermatogenesis; however, the reason for this is poorly understood. Recently, testis-specific protein Y-encoded 1 (TSPY1) copy number variation (CNV) was determined to be a potential genetic modifier of spermatogenesis. We performed a large-scale cohort study to investigate the effect of ... More
Gene expression profiling in insulinomas of Men1 beta-cell mutant mice reveals early genetic and epigenetic events involved in pancreatic beta-cell tumorigenesis.
Authors:Fontanière S, Tost J, Wierinckx A, Lachuer J, Lu J, Hussein N, Busato F, Gut I, Wang ZQ, Zhang CX,
Journal:Endocr Relat Cancer
PubMed ID:17158767
'Mutations of the MEN1 gene lead to the occurrence of multiple endocrine neoplasia type 1 (MEN1). To gain insights into the mechanisms of the tumorigenesis related to MEN1 inactivation, we have used mice in which the Men1 gene was specifically disrupted in pancreatic beta-cells. In these mice, we observed full ... More
Effects of essential oils on methane production and fermentation by, and abundance and diversity of, rumen microbial populations.
Authors:Patra AK, Yu Z,
Journal:Appl Environ Microbiol
PubMed ID:22492451
'Five essential oils (EOs), namely, clove oil (CLO), eucalyptus oil (EUO), garlic oil (GAO), origanum oil (ORO), and peppermint oil (PEO), were tested in vitro at 3 different doses (0.25, 0.50, and 1.0 g/liter) for their effect on methane production, fermentation, and select groups of ruminal microbes, including total bacteria, ... More
Normal variants of Microcephalin and ASPM do not account for brain size variability.
Authors:Woods RP, Freimer NB, De Young JA, Fears SC, Sicotte NL, Service SK, Valentino DJ, Toga AW, Mazziotta JC,
Journal:Hum Mol Genet
PubMed ID:16687438
'Normal human brain volume is heritable. The genes responsible for variation in brain volume are not known. Microcephalin (MCPH1) and ASPM (abnormal spindle-like microcephaly associated) have been proposed as candidate genes as mutations in both genes are associated with microcephaly, and common variants of each gene are apparently under strong ... More