Quant-iT™ Protein Assay Kit

Citations et références (6)

Invitrogen™

Quant-iT™ Protein Assay Kit

Le kit de dosage de protéines Quant-iT assure une analyse quantitative d’ADN facile et précise. Le kit comprend un réactifAfficher plus

Référence	Quantité
Q33210	1 kit

Référence Q33210

Prix (EUR)

926,00

Ajouter au panier

Quantité:

1 kit

Grand volume ou format personnalisé

Prix (EUR)

926,00

Ajouter au panier

Le kit de dosage de protéines Quant-iT assure une analyse quantitative d’ADN facile et précise. Le kit comprend un réactif d’essai concentré, un tampon de dilution et des étalons de BSA pré-dilués. Diluez simplement le réactif, chargez-le dans des puits de microplaque, ajoutez 1 à 20 µl d’échantillon, mélangez et mesurez la fluorescence. Le dosage est hautement sélectif pour les protéines et présente très peu de variations entre protéines. Le dosage est effectué à température ambiante et le signal est stable pendant 3 heures. Les contaminants courants, tels que les sels, les solvants ou l’ADN, mais pas les détergents, sont bien tolérés dans le dosage. Des kits de dosage d’ADN Quant-iT (Q33120, Q33130) et un kit de dosage d’ARN Quant-iT (Q33140) sont également disponibles.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

DoserDosage de protéines par fluorescence

À utiliser avec (équipement)Lecteur de microplaques

Identification génétique (Entrez)3919448

Contenus des kitsRéactif de dosage concentré, tampon de dilution et étalons de BSA prédilués

Gamme de produitsQuant-iT

Type de produitTest d’analyse quantitative des protéines

Quantité1 kit

Volume d’échantillon1 à 20 µl

Conditions d’expéditionTempérature ambiante

Conditions de stockageStocker au réfrigérateur (entre 2° et 8°C). Conserver à l’abri de la lumière.

Méthode de détectionFluorescence

Unit SizeEach

Contenu et stockage

Stocker au réfrigérateur (entre 2 et 8°C) à l’abri de la lumière.

Foire aux questions (FAQ)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

View all Quant-iT™ Protein Assay Kit FAQs

Citations et références (6)

Citations et références

Abstract

Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-kappaB1 DNA binding domain.

Authors:Lambert C, Li J, Jonscher K, Yang TC, Reigan P, Quintana M, Harvey J, Freed BM,

Journal:J Biol Chem

PubMed ID:17491020

Cigarette smoke is a potent inhibitor of pulmonary T cell responses, resulting in decreased immune surveillance and an increased incidence of respiratory tract infections. The alpha,beta-unsaturated aldehydes in cigarette smoke (acrolein and crotonaldehyde) inhibited production of interleukin-2 (IL-2), IL-10, granulocyte-macrophage colony-stimulating factor, interferon-gamma, and tumor necrosis factor-alpha by human T ... More

Functional selection of phagocytosis-promoting genes: cell sorting-based selection.

Authors:Jeon H, Go Y, Seo M, Lee WH, Suk K,

Journal:J Biomol Screen

PubMed ID:20660795

Phagocytosis is a critical host defense mechanism that clears invading pathogens, apoptotic cells, and cell debris; it is an essential process for normal development, tissue remodeling, immune response, and inflammation. Here, a functional selection strategy was used to isolate novel phagocytosis-promoting genes. After the retroviral transfer of mouse brain cDNA ... More

Quantitation of protein.

Authors:Noble JE, Bailey MJ,

Journal:Methods Enzymol

PubMed ID:19892168

The measurement of protein concentration in an aqueous sample is an important assay in biochemistry research and development labs for applications ranging from enzymatic studies to providing data for biopharmaceutical lot release. Spectrophotometric protein quantitation assays are methods that use UV and visible spectroscopy to rapidly determine the concentration of ... More

A comparison of protein quantitation assays for biopharmaceutical applications.

Authors:Noble JE, Knight AE, Reason AJ, Di Matola A, Bailey MJ,

Journal:Mol Biotechnol

PubMed ID:17914170

Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representing various sizes of protein and ... More

Targeted quantitation of overexpressed and endogenous cystic fibrosis transmembrane conductance regulator using multiple reaction monitoring tandem mass spectrometry and oxygen stable isotope dilution.

Authors:Jiang H, Ramos AA, Yao X,

Journal:Anal Chem

PubMed ID:19947594

Cystic fibrosis transmembrane conductance regulator (CFTR) functions as an ion channel in the apical plasma membrane of epithelial cells. Mutations in the gene coding for CFTR cause cystic fibrosis (CF). A major cellular dysfunction is insufficient apical plasma membrane expression of the protein. Its correction is important for developing new ... More

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