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Citations et références (5)
Invitrogen™

ProBond™ Nickel-Chelating Resin

La résine de chélation de nickelage ProBond™ est une résine d’affinité chargée en nickel utilisée pour purifier les protéines recombinantesAfficher plus
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RéférenceQuantité
R80115150 ml
R8010150 ml
Référence R80115
Prix (EUR)
2 475,00
Each
Quantité:
150 ml
Grand volume ou format personnalisé
Prix (EUR)
2 475,00
Each
La résine de chélation de nickelage ProBond™ est une résine d’affinité chargée en nickel utilisée pour purifier les protéines recombinantes contenant une séquence polyhistidique (6xHis). Les protéines liées à la résine peuvent être éluées avec un tampon à faible pH ou par compétition avec l’imidazole ou l’histidine. La purification en une étape peut être effectuée dans des conditions natives et dénaturantes. La résine ProBond™ contient de l’acide iminodiacétique de ligand chélatant associé à une résine d’agarose hautement réticulée à 6 % qui convient à une utilisation dans des applications de FPLC, de flux par lots et d’écoulement par gravité
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Quantité150 ml
Phase stationnaireChélation de nickelage
Type de colonneColonne d’affinité
FormeSuspension
Gamme de produitsProBond
TypeRésine
Unit SizeEach
Contenu et stockage
La résine ProBond™ est pré-chargée et peut se lier à 1 à 5 mg de protéine recombinante par 1 ml de résine. Elle est fournie sous forme de suspension à 50 % dans 20 % d’éthanol. La résine apparaît en bleu lorsqu’elle est chargée de Ni2+. Stockage à +4°C. La résine ProBond™ est garantie pendant 6 mois lorsqu’elle est entreposée correctement.

Foire aux questions (FAQ)

How do you typically detect expression of a recombinant fusion protein?

Western blot analysis is typically used to detect the expressed protein. We sell several antibodies against various epitopes, such as Xpress, HisG, V5, or C-terminal 6xHis. Additionally, His-tagged proteins can be purified using our ProBond Purification System via affinity purification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What expression levels can be expected with the pTrcHis/CAT construct?

In one experiment, 35 µg CAT/mg total protein or 68.2 mg CAT/liter of culture was obtained. 50 ml of cell extract was loaded onto a ProBond column and 2 mg of CAT was recovered. The eluted protein appeared reasonably pure on a gel.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

I purified my protein from a ProBond column using denaturing conditions. After elution, I tried digesting off my N- terminal tag with EKMax Enterokinase, but see no EK cleavage. What can you suggest I try?

The enzyme could be denatured. Try buffer exchange or dialysis before digestion with EKMax Enterokinase.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The pH of my ProBond buffers is off. Instead of pH 4-7, it is close to pH 9. What should I do?

pH drift is typical with these buffers. Adjust with concentrated HCl if the pH is too high or with 10 N NaOH if the pH is low.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all ProBond™ Nickel-Chelating Resin, 150 mL FAQs

Citations et références (5)

Citations et références
Abstract
Total synthesis of cyclic ADP-carbocyclic-ribose, a stable mimic of Ca2+-mobilizing second messenger cyclic ADP-ribose.
Authors: Shuto S; Fukuoka M; Manikowsky A; Ueno Y; Nakano T; Kuroda R; Kuroda H; Matsuda A;
Journal:J Am Chem Soc
PubMed ID:11535079
'The synthesis of cyclic ADP-carbocyclic-ribose (cADPcR, 4) designed as a stable mimic of cyclic ADP-ribose (cADPR, 1), a Ca2+-mobilizing second messenger, was achieved using as the key step a condensation reaction with the phenylthiophosphate-type substrate 14 to form an intramolecular pyrophosphate linkage. The N-1-carbocyclic-ribosyladenosine derivative 16 was prepared via the ... More
Interaction between the insulin receptor and its downstream effectors. Use of individually expressed receptor domains for structure/function analysis.
Authors:Paz K, Voliovitch H, Hadari YR, Roberts CT Jr, LeRoith D, Zick Y
Journal:J Biol Chem
PubMed ID:8636129
'A structural analysis has been carried out to determine which part of the intracellular domain of the insulin receptor (IR) beta subunit is involved in direct interaction with the receptor substrates IRS-1 and Shc. Toward this end, the juxtamembrane (JM) domain (amino acids 943- 984) and the carboxyl-terminal (CT) region ... More
A Calcium-Responsive Transcription Factor, CaRF, that Regulates Neuronal Activity-Dependent Expression of BDNF.
Authors: Tao Xu; West Anne E; Chen Wen G; Corfas Gabriel; Greenberg Michael E;
Journal:Neuron
PubMed ID:11832226
'Transcription of the brain-derived neurotrophic factor (BDNF) gene is regulated in a calcium- and neuron-selective manner; however, the mechanisms that underlie this selectivity are not known. We have characterized a new calcium-response element, CaRE1, that is required for activity-dependent transcription of BDNF exon III and have cloned a transcription factor, ... More
Tryptophan fluorescence reports nucleotide-induced conformational changes in a domain of the ArsA ATPase.
Authors:Zhou T, Rosen BP
Journal:J Biol Chem
PubMed ID:9242630
The ars operon of plasmid R773 encodes an ATP-dependent extrusion pump for arsenite and antimonite in Escherichia coli. The ArsA ATPase is the catalytic subunit of the pump protein, with two nucleotide binding consensus sequences, one in the NH2-terminal half and one in the COOH- terminal half of the protein. ... More
Structure and ligand of a histone acetyltransferase bromodomain.
Authors:Dhalluin C, Carlson JE, Zeng L, He C, Aggarwal AK, Zhou MM
Journal:Nature
PubMed ID:10365964
Histone acetylation is important in chromatin remodelling and gene activation. Nearly all known histone-acetyltransferase (HAT)-associated transcriptional co-activators contain bromodomains, which are approximately 110-amino-acid modules found in many chromatin-associated proteins. Despite the wide occurrence of these bromodomains, their three-dimensional structure and binding partners remain unknown. Here we report the solution structure ... More