SYTO™ Blue Fluorescent Nucleic Acid Stain Sampler Kit (SYTO™ dyes 40, 41, 42, 45)

Citations et références (2)

Invitrogen™

SYTO™ Blue Fluorescent Nucleic Acid Stain Sampler Kit (SYTO™ dyes 40, 41, 42, 45)

Comprend les colorants SYTO fluorescents bleus perméants aux cellules

Référence	Quantité
S11350	1 kit

Référence S11350

Prix (EUR)

676,00

Quantité:

1 kit

Prix (EUR)

676,00

Le kit d’échantillonneur de coloration d’acides nucléiques bleu fluorescent SYTO contient une collection de colorants d’acides nucléiques bleu fluorescent SYTO et perméants aux cellules (colorants SYTO® 40, 41, 42, 45). Comme les colorants peuvent présenter différents comportements de coloration avec divers tissus et cellules, il peut être nécessaire de tester les colorants pour trouver le colorant optimal pour une application spécifique. Le kit contient 50 µl de chaque colorant SYTO.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

CouleurBleu, Bleu

Méthode de détectionFluorescence, Fluorescent

Type de colorantPerméant aux cellules

Gamme de longueur d’onde d’excitationdivers

À utiliser avec (équipement)Microscope à fluorescence, Microscope à fluorescence

Gamme de produitsSYTO

Quantité1 kit

Conditions d’expéditionTempérature ambiante

Type d’étiquetteFluorescent Dye

Type de produitColoration d’acide nucléique

SubCellular LocalizationAcides nucléiques

Unit SizeEach

Contenu et stockage

Le kit contient 50 μl de chaque colorant SYTO.

Stocker au congélateur (entre -5°C et -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (2)

Citations et références

Abstract

Assessment of fluorochromes for two-photon laser scanning microscopy of biofilms.

Authors:Neu TR, Kuhlicke U, Lawrence JR

Journal:Appl Environ Microbiol

PubMed ID:11823234

A major limitation for the use of two-proton laser scanning microscopy (2P-LSM) in biofilm and other studies is the lack of a thorough understanding of the excitation-emission responses of potential fluorochromes. In order to use 2P-LSM, the utility of various fluorochromes and probes specific for a range of biofilm constituents ... More

Novel model for multispecies biofilms that uses rigid gas-permeable lenses.

Authors:Peyyala R, Kirakodu SS, Ebersole JL, Novak KF,

Journal:Appl Environ Microbiol

PubMed ID:21421785

Oral biofilms comprise complex multispecies consortia aided by specific inter- and intraspecies interactions occurring among commensals and pathogenic bacterial species. Oral biofilms are primary initiating factors of periodontal disease, although complex multifactorial biological influences, including host cell responses, contribute to the individual outcome of the disease. To provide a system ... More