SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680

Citations et références (5)

Invitrogen™

SAIVI™ Rapid Antibody Labeling Kit, Alexa Fluor™ 680

Le kit de marquage d’anticorps Alexa Fluor™ 680 SAIVI™ fournit un moyen pratique de marquer les anticorps avec un degréAfficher plus

Référence	Quantité
S30045	1 kit

Référence S30045

Prix (EUR)

920,00

Ajouter au panier

Quantité:

1 kit

Prix (EUR)

920,00

Ajouter au panier

Le kit de marquage d’anticorps Alexa Fluor™ 680 SAIVI™ fournit un moyen pratique de marquer les anticorps avec un degré optimal de marquage pour les applications d’imagerie in vivo (DOL ; ∼2) sur une plage de concentration d’anticorps de 6 fois sans qu’il soit nécessaire d’ajuster le volume de la réaction, la concentration du colorant ou la concentration des anticorps. Grâce à cette procédure, les anticorps marqués de manière optimale sont prêts pour les applications nécessitant des réactifs exempts d’azoture, tels que l’imagerie cellulaire en phase ou l’injection directe chez les animaux.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

CouleurIR

Méthode de détectionFluorescence

Excitation / émission679/702

Type d’étiquetteColorants Alexa Fluor

Balance d’étiquetage0,5 à 3 mg

Gamme de produitsAlexa Fluor, SAIVI

Type de produitKit de marquage d’anticorps

Quantité1 kit

Conditions d’expéditionTempérature ambiante

Labeling TargetAnticorps

Marqueur ou colorantAlexa Fluor 680

Unit SizeEach

Contenu et stockage

Conserver au réfrigérateur à 2°C à 8°C et à l’abri de la lumière.

Foire aux questions (FAQ)

What amount of conjugated antibody should I inject to image tumors?

A recommended starting dosage is 50 µg. You will need to determine the optimal dosage for your experimental model.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What size needle should I use for small animal in vivo imaging?

We recommend the use of a 28-32 gauge tuberculin or insulin syringe (0.3 or 1.0 mL volume) with a fixed (non-removable) needle.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the optimal and maximal volumes of reagent that can be injected into a mouse?

The volume of reagent that can be injected varies according to the route of administration. The following numbers are general guidelines for a 25 gram animal: Intravenous (IV)- 50-125 µl (recommended)- 200µl (maximum) ; Intraperitoneal (IP) 500µl (recommended) -2ml (maximum) ; Subcutaneous(SC) 100-250 µl (recommended)- 1ml (maximum).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of mice are the best for in vivo imaging?

Due to light scattering caused by fur, hairless mice such as athymic nude (nu/nu) mice are recommended for in vivo imaging. If this is not an option, the hair covering the area to be imaged should be removed using clippers or a chemical depilatory such as Nair depilatory.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (5)

Citations et références

Abstract

Affibody Molecules for In vivo Characterization of HER2-Positive Tumors by Near-Infrared Imaging.

Authors:Lee SB, Hassan M, Fisher R, Chertov O, Chernomordik V, Kramer-Marek G, Gandjbakhche A, Capala J,

Journal:Clin Cancer Res

PubMed ID:18559604

'PURPOSE: HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. We are developing molecular probes for in vivo quantitative imaging of HER2 receptors using near-infrared (NIR) optical imaging. The goal is to provide probes that will minimally interfere with the studied system, ... More

Noninvasive positron emission tomography and fluorescence imaging of CD133+ tumor stem cells.

Authors:Gaedicke S, Braun F, Prasad S, Machein M, Firat E, Hettich M, Gudihal R, Zhu X, Klingner K, Schüler J, Herold-Mende CC, Grosu AL, Behe M, Weber W, Mäcke H, Niedermann G,

Journal:

PubMed ID:24469819

'A technology that visualizes tumor stem cells with clinically relevant tracers could have a broad impact on cancer diagnosis and treatment. The AC133 epitope of CD133 currently is one of the best-characterized tumor stem cell markers for many intra- and extracranial tumor entities. Here we demonstrate the successful noninvasive detection ... More

Minimally invasive quantification of lymph flow in mice and rats by imaging depot clearance of near-infrared albumin.

Authors:Karlsen TV, McCormack E, Mujic M, Tenstad O, Wiig H,

Journal:Am J Physiol Heart Circ Physiol

PubMed ID:22101523

'There is a lack of available methods to noninvasively quantify lymphatic function in small experimental animals, a necessity for studies on lymphatic system pathophysiology. We present a new method to quantify lymph flow in mice and rats, based on optically monitoring the depot clearance of near-infrared fluorescently labeled albumin and ... More

Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.

Authors:McCormack E, Mujic M, Osdal T, Bruserud Ø, Gjertsen BT,

Journal:Blood

PubMed ID:23243270

Antibodies play a fundamental role in diagnostic immunophenotyping of leukemias and in cell-targeting therapy. However, this versatility is not reflected in imaging diagnostics. In the present study, we labeled anti–human mAbs monochromatically against selected human myeloid markers expressed on acute myeloid leukemia (AML) cells, all with the same near-infrared fluorochrome. ... More

Influenza virus aerosol exposure and analytical system for ferrets.

Authors:Gustin KM, Belser JA, Wadford DA, Pearce MB, Katz JM, Tumpey TM, Maines TR,

Journal:Proc Natl Acad Sci U S A

PubMed ID:21536880

Understanding the transmission ability of newly emerging influenza viruses is central to the development of public health preparedness and prevention strategies. Animals are used to model influenza virus infection and transmission, but the routinely used intranasal inoculation of a liquid virus suspension does not reflect natural infection. We report the ... More