Citations et références (11)

Invitrogen™

SYBR™ Safe DNA Gel Stain in 0.5X TBE

Name: SYBR™ Safe DNA Gel Stain in 0.5X TBE
SKU: S33101
Price: 714.00 EUR

Le colorant de gel d’ADN SYBR Safe a été développé spécifiquement pour une mutagénicité réduite – plus sûre que leAfficher plus

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Référence	Quantité
S33101	4 l
S33100	1 l

2 options

Référence S33101

Prix (EUR)

714,00

Each

Quantité:

4 l

Grand volume ou format personnalisé

Prix (EUR)

714,00

Each

Le colorant de gel d’ADN SYBR Safe a été développé spécifiquement pour une mutagénicité réduite – plus sûre que le bromure d’éthidium pour la coloration de l’ADN dans les gels d’agarose ou d’acrylamide La coloration SYBR Safe n’est pas seulement moins mutagène que le bromure d’éthidium, mais sa sensibilité de détection est meilleure que celle du bromure d’éthidium. La coloration SYBR Safe est une solution prémélangée qui peut être utilisée de la même manière qu’une solution de bromure d’éthidium, soit dans le gel pendant le cycle d’électrophorèse, soit comme coloration post-cycle. Lié aux acides nucléiques, le colorant vert fluorescent SYBR Safe affiche un maximum d’excitation de fluorescence d’environ 280 et environ 502 nm, ainsi qu’un maximum d’émission de la fluorescence d’environ 530 nm. Le colorant de gel d’ADN SYBR Safe est également disponible au format 1 litre (S-33100) de solution prête à l’emploi. Le kit de démarrage de colorant de gel d’ADN SYBR Safe (S-33110) comprend 1 litre de colorant de gel d’ADN SYBR Safe et un filtre photographique (S-37100).

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Emplacement de détectionDétection sur gel

Méthode de détectionFluorescence

Quantité4 l

Durée de conservation6 mois

Conditions d’expéditionTempérature ambiante

Molécule cibleADN

Marqueur ou colorantSYBR Safe

Type de produitColoration sur gel d’ADN

Unit SizeEach

Contenu et stockage

• Fourni sous la forme d’une solution prête à l’emploi dans du TBE 0,5 X

Conserver à température ambiante dans le récipient d’origine.

Foire aux questions (FAQ)

Why do I sometimes see speckles in my gel when using SYBR Safe DNA Gel Stain?

Many whitening agents used in clothing, as well as some fungi and bacteria, fluoresce at the same wavelengths as SYBR Safe DNA gel stain. These contaminants within or on the surface of the gel may produce this speckling.

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Which direction does the SYBR Safe dye run during electrophoresis?

Similarly to ethidium bromide, SYBR Safe DNA Gel Stain runs in the opposite direction of the migrating DNA. This has no practical effect on the use of gels cast with SYBR Safe DNA Gel Stain, as only the very bottom of the gel will have a lower concentration of stain. This effect can be partially counteracted by staining the gel with SYBR Safe DNA Gel Stain after electrophoresis. Solutions of dye should not be added to the running buffer as this can cause breakdown of the dye at the electrodes and release toxic volatile compounds into the air.

Does ethanol precipitation remove the SYBR Safe dye?

SYBR Safe DNA Gel Stain is easily removed from nucleic acids by ethanol precipitation.

Can I reuse SYBR Safe DNA Gel Stain for a second gel?

We strongly discourage the reuse of SYBR Safe DNA Gel Stain, as this practice significantly lowers sensitivity.

View all SYBR™ Safe DNA Gel Stain in 0.5X TBE, 4 L FAQs

Citations et références (11)

Citations et références

Abstract

Features of medullary thymic epithelium implicate postnatal development in maintaining epithelial heterogeneity and tissue-restricted antigen expression.

Authors:Gillard GO, Farr AG

Journal:J Immunol

PubMed ID:16670287

'Although putative thymic epithelial progenitor cells have been identified, the developmental potential of these cells, the extent of medullary thymic epithelium (mTEC) heterogeneity, and the mechanisms that mediate the expression of a wide range of peripheral tissue-restricted Ags (TRAs) by mTECs remain poorly defined. Here we have defined several basic ... More

Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports.

Authors:Handwerger RG, Diamond SL

Journal:Bioconjug Chem

PubMed ID:17432825

'A biotinylated photocleavable polyethylenimine (B-PC-PEI) was designed and synthesized for the capture and controlled release of nucleic acids from solid supports. B-PC-PEI was synthesized via a three-step reaction process and verified by 1H NMR and mass spectrometry. In aqueous solution, the o-nitrobenzyl group within B-PC-PEI was efficiently cleaved by 5 ... More

Development of a SYBR safe technique for the sensitive detection of DNA in cesium chloride density gradients for stable isotope probing assays.

Authors:Martineau C, Whyte LG, Greer CW,

Journal:J Microbiol Methods

PubMed ID:18329741

SYBR safe, a fluorescent nucleic acid stain, was evaluated as a replacement for ethidium bromide (EtBr) in cesium chloride (CsCl) density gradients for DNA stable isotope probing (DNA-SIP) assays. The separation of 12C- and 13C-labelled DNA using SYBR safe gave similar results to those obtained using EtBr with pure cultures ... More

Single step protocol to purify recombinant proteins with low endotoxin contents.

Authors:Reichelt P, Schwarz C, Donzeau M

Journal:Protein Expr Purif

PubMed ID:16290005

Endotoxin is an unwanted by product of recombinant proteins purified from Escherichia coli. The inherent toxicity of endotoxins makes their removal an important step for the proteins' application in several biological assays and for safe parenteral administration. The method described in this paper is a one-step protocol which is effective ... More

5-Methyltetrahydrofolate inhibits photosensitization reactions and strand breaks in DNA.

Authors:Offer T, Ames BN, Bailey SW, Sabens EA, Nozawa M, Ayling JE

Journal:FASEB J

PubMed ID:17341682

The known functions of folate are to support one-carbon metabolism and to serve as photoreceptors for cryptochromes and photolyases. We demonstrate that 5-methyltetrahydrofolate (5-MTHF, the predominant folate in plasma) is also a potent, near diffusion limited, scavenger of singlet oxygen and quencher of excited photosensitizers. Both pathways result in decomposition ... More

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