Coloration pour gel de protéines SYPRO™

Citations et références (112)

Invitrogen™

Coloration pour gel de protéines SYPRO™

Name: Coloration pour gel de protéines SYPRO™
SKU: S6651
Price: 498.65 EUR

Le colorant du gel de protéines SYPRO Ruby est fluorescent, prêt à l’emploi et extrêmement sensible. Il permet de détecterAfficher plus

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Référence	Couleur	Quantité
S6651	Orange	10 x 50 μl
S6650	Orange	500 μl
S12010	Mandarine	500 μl
S6653	Rouge	500 μl
S6654 également connu sous le numéro S-6654	Rouge	10 x 50 μl
S12001	Rubis	200 ml
S12000	Rubis	1 l
S21900	Rubis	5 l
S12000X3	Rubis	3 x 1 litre

9 options

Référence S6651

Prix (EUR)

498,65

線上優惠

534,00

Économisez 35,35 (7%)

Each

Ajouter au panier

Couleur:

Orange

Quantité:

10 x 50 μl

Grand volume ou format personnalisé

Prix (EUR)

498,65

線上優惠

534,00

Économisez 35,35 (7%)

Each

Ajouter au panier

Le colorant du gel de protéines SYPRO Ruby est fluorescent, prêt à l’emploi et extrêmement sensible. Il permet de détecter des protéines totales séparées par l’électrophorèse sur gel de polyacrylamide (PAGE). Il convient parfaitement pour un usage en PAGE 1D et 2D. La sensibilité de coloration du gel SYPRO Ruby est aussi bonne, voire meilleure que les meilleures techniques de coloration à l’argent. Les protéines colorées peuvent être visualisées à l’aide d’un transilluminateur UV ou lumière bleue standard ou d’un équipement d’imagerie contenant les filtres ou les lasers appropriés.

Caractéristiques :
• Procédure de coloration simple : aucune étape de décoloration ou chronométrage requis
• Gamme de quantification linéaire sur trois ordres de grandeur
• Compatibilité avec la spectrométrie de masse et le microséquençage

Comparez tous les colorants fluorescents ›

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Concentration5 000X dans du DMSO

Emplacement de détectionDétection sur gel

Méthode de détectionFluorescence

Excitation / émission300, 470/570 nm

Gamme de produitsSYPRO

Type de produitColoration sur gel des protéines

Quantité10 x 50 μl

Conditions d’expéditionTempérature ambiante

Molécule cibleProtéine

CouleurOrange

Marqueur ou colorantSYPRO Orange

Unit SizeEach

Contenu et stockage

Fourni sous la forme d’un concentrat de 5000X dans le DMSO.Stocker à température ambiante et à l’abri de la lumière.

Foire aux questions (FAQ)

If I increase the final concentration of my SYPRO Orange or SYPRO Red staining solution above 1X, can I increase the signal of my stained proteins?

No. Dye concentrations higher than 1X do not give better detection. Instead, background fluorescence increases and, as the dye concentration increases, the dye becomes self-quenching and the signal actually decreases.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I dry my SYPRO Ruby, SYPRO Orange, or SYPRO Red stained gels?

Gels stained with SYPRO dyes can be dried between sheets of cellophane, although there is sometimes a slight decrease in sensitivity. If the gels are dried onto paper, the light will scatter and the sensitivity will decrease. Other plastics are not recommended, as the plastic typically used is not transparent to UV light.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I pre-stain proteins with SYPRO Ruby, SYPRO Orange or SYPRO Red Protein Gel Stains and then run them through a gel?

No. Loading solutions contain so much SDS that SYPRO Ruby, SYPRO Orange and SYPRO Red dyes simply localize in the free SDS and bind very little of the proteins. Proteins can be covalently pre-labeled with ATTO-TAG CBQCA (Cat. No. A2333), DDAO succinimidyl ester (Cat. No. C34553) or TAMRA-succinimidyl ester (Cat. No. C2211) dyes, or the TC-FLAsH Expression Analysis Detection Kits (Cat. No. A10067 for orange fluorescence, Cat. No. A10068 for red fluorescence) prior to electrophoresis without affecting protein migration through the gel.

SYPRO Orange or SYPRO Red Protein Gel Stain can be diluted 5000-fold into the cathode (upper) buffer tank to stain proteins during electrophoresis without affecting migration. The problem with doing this is that there is considerable background fluorescence in the gels from the dye interacting with SDS. This can be reduced after electrophoresis by destaining in 7.5% acetic acid for 15-60 minutes. This method also results in poorer protein sensitivity than the standard post-staining method, requires the same amount of time before the gel can be imaged, and contaminates the electrophoresis apparatus.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Citations et références (112)

Citations et références

Abstract

LXXLL-related motifs in Dax-1 have target specificity for the orphan nuclear receptors Ad4BP/SF-1 and LRH-1.

Authors:Suzuki T, Kasahara M, Yoshioka H, Morohashi K, Umesono K

Journal:Mol Cell Biol

PubMed ID:12482977

The orphan receptor Ad4BP/SF-1 (NR5A1) is a constitutive activator, and its activity is repressed by another orphan receptor, Dax-1 (NR0B1). In the present study, we investigated the molecular mechanisms underlying this repression by Dax-1. Yeast two-hybrid and transient-transfection assays confirmed the necessity of three LXXLL-related motifs in Dax-1 for interaction ... More

Translational regulation of prostaglandin endoperoxide H synthase-1 mRNA in megakaryocytic MEG-01 cells. Specific protein binding to a conserved 20-nucleotide CIS element in the 3'-untranslated region.

Authors:Duquette M, Laneuville O

Journal:J Biol Chem

PubMed ID:12237309

Prostaglandin endoperoxide H synthase-1 (PGHS-1) is an abundant enzyme in platelets, where it plays a key role in the cascade of prostanoid formation. In platelets, the primary site of PGHS-1 synthesis is in precursor megakaryocytic cells. We have previously shown that in megakaryocytic MEG-01 cells, TPA induces an increase of ... More

Type I collagen is thermally unstable at body temperature.

Authors:Leikina E, Mertts MV, Kuznetsova N, Leikin S

Journal:Proc Natl Acad Sci U S A

PubMed ID:11805290

Molecular characterization of Saccharomyces cerevisiae TFIID.

Authors:Sanders SL, Garbett KA, Weil PA

Journal:Mol Cell Biol

PubMed ID:12138208

'We previously defined Saccharomyces cerevisiae TFIID as a 15-subunit complex comprised of the TATA binding protein (TBP) and 14 distinct TBP-associated factors (TAFs). In this report we give a detailed biochemical characterization of this general transcription factor. We have shown that yeast TFIID efficiently mediates both basal and activator-dependent transcription ... More

Mechanism of calcium-independent synaptotagmin binding to target SNAREs.

Authors:Rickman C, Davletov B

Journal:J Biol Chem

PubMed ID:12496268

'Synaptic vesicle exocytosis requires three SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) proteins: syntaxin and SNAP-25 on the plasma membrane (t-SNAREs) and synaptobrevin/VAMP on the synaptic vesicles (v-SNARE). Vesicular synaptotagmin 1 is essential for fast synchronous SNARE-mediated exocytosis and interacts with the SNAREs in brain material. To uncover the step at ... More

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