Sodium Green™ Tetraacetate, cell permeant - Special Packaging

Citations et références (35)

Invitrogen™

Sodium Green™ Tetraacetate, cell permeant - Special Packaging

Cette forme perméante cellulaire de tétraacétate Sodium Green™ est un indicateur de Na+ visible et excitable par la lumière.En savoirAfficher plus

Référence	Quantité
S6901	20 x 50 μg

Référence S6901

Prix (EUR)

758,00

Ajouter au panier

Quantité:

20 x 50 μg

Prix (EUR)

758,00

Ajouter au panier

Cette forme perméante cellulaire de tétraacétate Sodium Green™ est un indicateur de Na⁺ visible et excitable par la lumière.

En savoir plus sur les indicateurs d’ions, notamment les indicateurs de calcium, de potassium, de pH et potentiel de membrane ›

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Méthode de détectionFluorescence

Type de colorantIndicateur de sodium

Quantité20 x 50 μg

Conditions d’expéditionTempérature ambiante

À utiliser avec (application)Viabilité et prolifération des cellules

À utiliser avec (équipement)Microscope à fluorescence

Gamme de produitsSodium Green

Type de produitIndicateur de sodium

Unit SizeEach

Contenu et stockage

Conservez au congélateur (entre -5 et -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (35)

Citations et références

Abstract

Expression of the voltage-gated sodium channel NaV1.5 in the macrophage late endosome regulates endosomal acidification.

Authors:Carrithers MD, Dib-Hajj S, Carrithers LM, Tokmoulina G, Pypaert M, Jonas EA, Waxman SG,

Journal:J Immunol

PubMed ID:17548620

'Voltage-gated sodium channels expressed on the plasma membrane activate rapidly in response to changes in membrane potential in cells with excitable membranes such as muscle and neurons. Macrophages also require rapid signaling mechanisms as the first line of defense against invasion by microorganisms. In this study, our goal was to ... More

Interaction of cofilin with triose-phosphate isomerase contributes glycolytic fuel for Na,K-ATPase via Rho-mediated signaling pathway.

Authors:Jung J, Yoon T, Choi EC, Lee K

Journal:J Biol Chem

PubMed ID:12359716

'We reported previously that cofilin, an actin-binding protein, interacts with Na,K-ATPase and enhances its activity (Lee, K., Jung, J., Kim, M., and Guidotti, G. (2001) Biochem. J. 353, 377-385). To understand the nature of this interaction and the role of cofilin in the regulation of Na,K-ATPase activity, we searched for ... More

Na+-dependent release of Mg2+ from an intracellular pool in rat sublingual mucous acini.

Authors:Zhang GH, Melvin JE

Journal:J Biol Chem

PubMed ID:8910560

'Muscarinic stimulation induces release of Mg2+ from an intracellular pool in rat sublingual mucous acini (Zhang, G. H., and Melvin, J. E. (1992) J. Biol. Chem. 267, 20721-20727). In the present study we examined the interdependence of Mg2+ mobilization on intracellular Na+ and Ca2+ by monitoring the intracellular free concentrations ... More

Pleural surface fluorescence measurement of Na+ and Cl- transport across the air space-capillary barrier.

Authors:Jiang J, Song Y, Bai C, Koller BH, Matthay MA, Verkman AS

Journal:J Appl Physiol

PubMed ID:12391048

'We developed a pleural surface fluorescence method to measure Na(+) and Cl(-) transport in perfused mouse lungs. The air space was filled with aqueous fluid containing membrane-impermeant fluorescent indicators of Cl(-) (lucigenin) or Na(+) (Sodium Green). After instillation of a Cl(-)-free solution into the air space, an increase in perfusate ... More

Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells.

Authors:Lo CJ, Leake MC, Berry RM

Journal:Biophys J

PubMed ID:16227503

'The energy-transducing cytoplasmic membrane of bacteria contains pumps and antiports maintaining the membrane potential and ion gradients. We have developed a method for rapid, single-cell measurement of the internal sodium concentration ([Na(+)](in)) in Escherichia coli using the sodium ion fluorescence indicator, Sodium Green. The bacterial flagellar motor is a molecular ... More

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