Coloration de gel d’ARN SYBR™ Green II, concentrat 10 000X dans le DMSO
Coloration de gel d’ARN SYBR™ Green II, concentrat 10 000X dans le DMSO
Citations et références (34)
Invitrogen™

Coloration de gel d’ARN SYBR™ Green II, concentrat 10 000X dans le DMSO

Le colorant de gel d’ARN SYBR Green II est un colorant de gel sensible à l’acide nucléique dont la fluorescenceAfficher plus
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RéférenceQuantité
S7564500 μl
S75681 ml
S758620 x 50 μl
Référence S7564
Prix (EUR)
590,00
Each
Ajouter au panier
Quantité:
500 μl
Grand volume ou format personnalisé
Prix (EUR)
590,00
Each
Ajouter au panier
Le colorant de gel d’ARN SYBR Green II est un colorant de gel sensible à l’acide nucléique dont la fluorescence est brillante lorsqu’elle est liée à l’ARN et à un fond faible dans les gels, ce qui le rend idéal pour une utilisation avec des gels de formaldéhyde/agarose ou de polyacrylamide à l’aide de scanners laser ou de transilluminateurs UV standard. Une taille d’unité de 500 µl (S-7564) et une taille d’unité de 1 ml (S-7568) sont disponibles.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Emplacement de détectionDétection sur gel
Méthode de détectionFluorescence
Type de produitColoration sur gel d’ARN
Quantité500 μl
Conditions d’expéditionTempérature ambiante
Molécule cibleARN
Marqueur ou colorantSYBR Vert II
Unit SizeEach
Contenu et stockage
• Fourni sous forme de concentré 10 000 X dans du DMSO

Conserver à -20°C, à l’abri de la lumière dans un dessiccateur.

Foire aux questions (FAQ)

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the recommended filter for my gel documentation system?

Please go here (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/dna-stains/sybr-safe.html) and click on the “Filter Recommendations” tab to see filter recommendations for use with SYBR Safe DNA Gel Stain. Note that the excitation and emission spectra of SYBR Safe DNA Gel Stain are very similar to those of SYBR Green I, SYBR Green II, and SYBR Gold dyes, as well as fluorescein (FITC). Therefore, filters appropriate for these dyes can also be used. A camera filter is not required with the Safe Imager Blue-Light Transilluminator; the amber filter provided with the instrument serves this purpose.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I use the ethidium bromide filters on my camera to image SYBR dyes?

This is not recommended. Most deep amber/orange ethidium bromide filters have a cutoff value around 550 nm. The SYBR Green dyes emit at 520 nm, which will not be detected using this filter.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Is SYBR Safe DNA Gel Stain the same as SYBR Green I dye?

All SYBR dyes have similar spectral properties, but have different chemical compositions. All SYBR dyes bind to dsDNA, ssDNA and RNA but vary in sensitivity. SYBR Safe DNA Gel Stain (Cat. No. S33102) was specifically developed as a safer alternative to ethidium bromide. SYBR Green I (Cat. No. S7585) is an ultrasensitive stain for dsDNA, and SYBR Green II (Cat. No. S7564) is a highly sensitive stain for RNA and ssDNA. All SYBR dyes are optimally excited by the Safe Imager Blue-Light Transilluminator.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What are the differences between all the SYBR dyes?

Different SYBR dyes bind to dsDNA, ssDNA, and RNA, but vary in the sensitivity and specificity with which they bind to different nucleic acids. SYBR Green I Nucleic Acid Gel Stain is used for staining dsDNA and ssDNA. SYBR Green II RNA Gel Stain will stain dsDNA and ssDNA but has better sensitivity for RNA. SYBR Gold Acid Gel Stain was developed after SYBR Green I and II and is the most sensitive fluorescent gel stain offering the highest sensitivity for both DNA and RNA. SYBR Safe DNA Gel Stain is a reduced mutagenicity formula designed for use with blue light systems. It is less sensitive than the SYBR Green I and II but comparable to ethidium bromide.

Please check the following link for additional details regarding the different SYBR dyes: The Molecular Probes Handbook: Nucleic Acid Detection on Gels, Blots and Arrays

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

View all Coloration de gel d’ARN SYBR™ Green II, concentrat 10 000X dans le DMSO FAQs

Citations et références (34)

Citations et références
Abstract
Authors:
Journal:
PubMed ID:8660526
SPACRCAN, a novel human interphotoreceptor matrix hyaluronan-binding proteoglycan synthesized by photoreceptors and pinealocytes.
Authors:Acharya S, Foletta VC, Lee JW, Rayborn ME, Rodriguez IR, Young WS, Hollyfield JG
Journal:J Biol Chem
PubMed ID:10702256
The interphotoreceptor matrix is a unique extracellular complex occupying the interface between photoreceptors and the retinal pigment epithelium in the fundus of the eye. Because of the putative supportive role in photoreceptor maintenance, it is likely that constituent molecules play key roles in photoreceptor function and may be targets for ... More
Lateral-flow and up-converting phosphor reporters to detect single-stranded nucleic acids in a sandwich-hybridization assay.
Authors:Corstjens PL, Zuiderwijk M, Nilsson M, Feindt H, Sam Niedbala R, Tanke HJ
Journal:Anal Biochem
PubMed ID:12531205
'Up-converting Phosphor Technology (UPT) particles were used as reporters in lateral-flow (LF) assays to detect single-stranded nucleic acids. The 400-nm phosphor particles exhibit strong visible luminescence upon excitation with infrared (IR) light resulting in the total absence of background autofluorescence from other biological compounds. A sandwich-type hybridization assay was applied ... More
Application of digital image analysis and flow cytometry to enumerate marine viruses stained with SYBR gold.
Authors:Chen F, Lu JR, Binder BJ, Liu YC, Hodson RE
Journal:Appl Environ Microbiol
PubMed ID:11157214
'A novel nucleic acid stain, SYBR Gold, was used to stain marine viral particles in various types of samples. Viral particles stained with SYBR Gold yielded bright and stable fluorescent signals that could be detected by a cooled charge-coupled device camera or by flow cytometry. The fluorescent signal strength of ... More
The stability of tristetraprolin mRNA is regulated by mitogen-activated protein kinase p38 and by tristetraprolin itself.
Authors:Tchen CR, Brook M, Saklatvala J, Clark AR
Journal:J Biol Chem
PubMed ID:15187092
'Tristetraprolin (TTP) is an mRNA-destabilizing protein that negatively regulates the expression of proinflammatory mediators such as tumor necrosis factor alpha, granulocyte/macrophage colony-stimulating factor, and cyclooxygenase 2. Here we investigate the regulation of TTP expression in the mouse macrophage cell line RAW264.7. We show that TTP mRNA is expressed in a ... More
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