Colorant JC-1 (sonde de potentiel de membrane mitochondriale)

Citations et références (400)

Invitrogen™

Colorant JC-1 (sonde de potentiel de membrane mitochondriale)

JC-1 est un nouveau colorant de carbocyanine cationique qui s’accumule dans les mitochondries. Le colorant existe sous forme de monomèreAfficher plus

Référence	Quantité
T3168	5 mg

Référence T3168

Prix (EUR)

855,65

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930,00

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Quantité:

Prix (EUR)

855,65

Online Exclusive

930,00

Économisez 74,35 (8%)

Ajouter au panier

JC-1 est un nouveau colorant de carbocyanine cationique qui s’accumule dans les mitochondries. Le colorant existe sous forme de monomère à basses concentrations et donne une fluorescence verte, similaire à celle de la fluorescéine. À des concentrations plus élevées, le colorant forme des agrégats J qui présentent un large spectre d’excitation et une émission maximum à environ 590 nm. Ces caractéristiques font de JC-1 un marqueur sensible pour le potentiel de membrane mitochondriale. JC-9 (D-22421) est un autre colorant présentant des caractéristiques similaires.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Méthode de détectionFluorescence

Quantité5 mg

Conditions d’expéditionTempérature ambiante

Localisation subcellulaireMitochondries

CouleurVert

À utiliser avec (équipement)Microscope à fluorescence, Cytomètre en flux

Type de produitColorant

Unit Size5 mg

Contenu et stockage

Stocker à température ambiante et à l’abri de la lumière.

Foire aux questions (FAQ)

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (400)

Citations et références

Abstract

Apoptosis induced by Rac GTPase correlates with induction of FasL and ceramides production.

Authors:Embade N,Valerón PF,Aznar S,López-Collazo E,Lacal JC

Journal:Molecular biology of the cell

PubMed ID:11102528

Rho proteins, members of the Ras superfamily of GTPases, are critical elements in signal transduction pathways governing cell proliferation and cell death. Different members of the family of human Rho GTPases, including RhoA, RhoC, and Rac1, participate in the regulation of apoptosis in response to cytokines and serum deprivation in ... More

Large-scale chemical dissection of mitochondrial function.

Authors:Wagner BK,Kitami T,Gilbert TJ,Peck D,Ramanathan A,Schreiber SL,Golub TR,Mootha VK

Journal:Nature biotechnology

PubMed ID:18297058

Mitochondrial oxidative phosphorylation (OXPHOS) is central to physiology and disease pathogenesis. To systematically investigate its activity and regulation, we performed a wide range of assays of OXPHOS physiology and nuclear and mitochondrial gene expression across 2490 chemical perturbations in muscle cells. Through mining of the resulting compendium, we discovered that: ... More

Authors:

Journal:

PubMed ID:10891486

Mitochondria--potential role in cell life and death.

Authors:Griffiths EJ

Journal:Cardiovascular research

PubMed ID:10727650

The mitochondrial death/life regulator in apoptosis and necrosis.

Authors:Kroemer G,Dallaporta B,Resche-Rigon M

Journal:Annual review of physiology

PubMed ID:9558479

Both physiological cell death (apoptosis) and, in some cases, accidental cell death (necrosis) involve a two-step process. At a first level, numerous physiological and some pathological stimuli trigger an increase in mitochondrial membrane permeability. The mitochondria release apoptogenic factors through the outer membrane and dissipate the electrochemical gradient of the ... More

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