Invitrogen™

ZOOM™ Carrier Ampholytes 4-6, 10mL

Les ampholytes porteurs ZOOM® sont de petites molécules solubles avec des groupes de charge positive et négative. Ils trient enAfficher plus

Référence	Quantité
ZM0025	10 ml

Référence ZM0025

Prix (EUR)

504,00

10 mL

Quantité:

10 ml

Prix (EUR)

504,00

10 mL

Les ampholytes porteurs ZOOM® sont de petites molécules solubles avec des groupes de charge positive et négative. Ils trient en fonction de leurs points isoélectriques dans un champ électrique, établissant ainsi un gradient de pH dans la solution ou contribuant ainsi au gradient lorsqu’ils sont utilisés avec des bandes IPG. Les ampholytes porteurs aident à stabiliser le gradient de pH et le courant dans les bandes IPG et à la solubilité des protéines, ce qui donne une résolution IEF reproductible.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Quantité10 ml

Durée de conservation1 an

Conditions d’expéditionTempérature ambiante

Gamme de produitsZOOM

Type de produitAmpholyte porteur

Plage de pH4 à 6

Unit Size10 mL

Contenu et stockage

Les ampholytes porteurs ZOOM™ sont fournis en flacons de 10 ml. Stocker à +4°C. Stabilité garantie pendant 1 an sous réserve d’une bonne conservation.

Foire aux questions (FAQ)

When running the ZOOM IEF Fractionator, if there is not a solubility issue, will the proteins/peptides in the sample migrate to their expected chambers based on isoelectric point in the absence of carrier ampholytes? Won't they act as ampholytes within each chamber?

Proteins are ampholytes, but in general they are poor carrier ampholytes. The profiles of the fractions are similar, but not identical, to those obtained with ampholytes in the sample.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I heat my protein sample prepared using ZOOM 2D Protein Solubilizer?

We do not recommend heating protein samples containing urea over 37 degrees C as elevated temperatures cause urea to hydrolyze to isocyanate, which modifies proteins by carbamylation.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How do you recommend storing protein samples prepared in the Sample Rehydration buffer?

We recommend storing them at -80 degrees C. We do not recommend storing them at -20 degrees C

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How much ampholyte do you recommend adding to the sample rehydration buffer?

The recommended ampholyte concentration in the sample rehydration buffer is 0.5%.

*If you are loading 5-50 µg of protein (pure protein or crude lysate) per ZOOM strip, use 0.5% ampholytes in the sample rehydration buffer.
*If you are loading >50 µg of protein (crude lysate or fractionated sample) per ZOOM Strip, use 0.5-2% ampholytes in the sample rehydration buffer.

Note: Higher ampholyte concentration requires longer focusing times.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the maximum recommended volume of protein sample to be added to the Sample Rehydration buffer?

The maximum volume of the protein sample should at most be 1/6 of the final sample volume that will be added to the strip. A good general volume would be 5-10µL. 140 µL of sample diluted in Sample Rehydration buffer is used to rehydrate each ZOOM Strip for the standard rehydration time of one hour. For overnight rehydration, we recommend using 155 µL of diluted sample.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all ZOOM™ Carrier Ampholytes 4-6, 10mL FAQs